Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause
<i>Sitodiplosis mosellana</i>, a periodic but devastating wheat pest that escapes temperature extremes in summer and winter by undergoing obligatory diapause. To determine the roles of small heat shock proteins (sHsps) in diapause of <i>S. mosellana,</i> we characterized two...
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MDPI AG
2021-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/2/119 |
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| author | Jiajia Zhao Qitong Huang Guojun Zhang Keyan Zhu-Salzman Weining Cheng |
| author_facet | Jiajia Zhao Qitong Huang Guojun Zhang Keyan Zhu-Salzman Weining Cheng |
| author_sort | Jiajia Zhao |
| collection | DOAJ |
| description | <i>Sitodiplosis mosellana</i>, a periodic but devastating wheat pest that escapes temperature extremes in summer and winter by undergoing obligatory diapause. To determine the roles of small heat shock proteins (sHsps) in diapause of <i>S. mosellana,</i> we characterized two <i>sHsp</i> genes, <i>SmHsp17.4</i> and <i>SmHsp20.3</i>, from this species. Both SmHsps contained the conserved α-crystallin domain and the carboxy-terminal I/VXI/V motif of the sHsp family. <i>SmHsp17.4</i> had one intron while <i>SmHsp20.3</i> had none. Quantitative PCR revealed that <i>SmHsp17.4</i> expression decreased after diapause initiation, but substantially increased during transition to post-diapause quiescence. In contrast, <i>SmHsp20.3</i> expression was not affected by entry of diapause, but was clearly up-regulated during summer and winter. Short-term more severe heat-stress (≥35 °C) of over-summering larvae or cold-stress (≤−5 °C) of over-wintering larvae could stimulate higher expression of both genes, and <i>SmHsp17.4</i> was more responsive to cold stress while <i>SmHsp20.3</i> was more sensitive to heat stress. Notably, transcription of <i>SmHsp17.4</i>, but not <i>SmHsp20.3</i>, in diapausing larvae was inducible by 20-hydroxyecdysone (20E). Recombinant SmHsp17.4 and SmHsp20.3 proteins also displayed significant chaperone functionality. These findings suggest that both <i>SmHsps</i> play key roles in stress tolerance during diapause; and 20E-regulated <i>SmHsp17.4</i> was also likely involved in diapause termination. |
| first_indexed | 2024-03-09T03:18:14Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T03:18:14Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj.art-b16d746673e341d7880517a6d12511492023-12-03T15:15:27ZengMDPI AGInsects2075-44502021-01-0112211910.3390/insects12020119Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during DiapauseJiajia Zhao0Qitong Huang1Guojun Zhang2Keyan Zhu-Salzman3Weining Cheng4Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaDepartment of Entomology, Texas A&M University, College Station, TX 77843, USAKey Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, China<i>Sitodiplosis mosellana</i>, a periodic but devastating wheat pest that escapes temperature extremes in summer and winter by undergoing obligatory diapause. To determine the roles of small heat shock proteins (sHsps) in diapause of <i>S. mosellana,</i> we characterized two <i>sHsp</i> genes, <i>SmHsp17.4</i> and <i>SmHsp20.3</i>, from this species. Both SmHsps contained the conserved α-crystallin domain and the carboxy-terminal I/VXI/V motif of the sHsp family. <i>SmHsp17.4</i> had one intron while <i>SmHsp20.3</i> had none. Quantitative PCR revealed that <i>SmHsp17.4</i> expression decreased after diapause initiation, but substantially increased during transition to post-diapause quiescence. In contrast, <i>SmHsp20.3</i> expression was not affected by entry of diapause, but was clearly up-regulated during summer and winter. Short-term more severe heat-stress (≥35 °C) of over-summering larvae or cold-stress (≤−5 °C) of over-wintering larvae could stimulate higher expression of both genes, and <i>SmHsp17.4</i> was more responsive to cold stress while <i>SmHsp20.3</i> was more sensitive to heat stress. Notably, transcription of <i>SmHsp17.4</i>, but not <i>SmHsp20.3</i>, in diapausing larvae was inducible by 20-hydroxyecdysone (20E). Recombinant SmHsp17.4 and SmHsp20.3 proteins also displayed significant chaperone functionality. These findings suggest that both <i>SmHsps</i> play key roles in stress tolerance during diapause; and 20E-regulated <i>SmHsp17.4</i> was also likely involved in diapause termination.https://www.mdpi.com/2075-4450/12/2/119<i>Sitodiplosis mosellana</i><i>Hsp17.4</i><i>Hsp20.3</i>diapausestress toleranceecdysone |
| spellingShingle | Jiajia Zhao Qitong Huang Guojun Zhang Keyan Zhu-Salzman Weining Cheng Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause Insects <i>Sitodiplosis mosellana</i> <i>Hsp17.4</i> <i>Hsp20.3</i> diapause stress tolerance ecdysone |
| title | Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause |
| title_full | Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause |
| title_fullStr | Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause |
| title_full_unstemmed | Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause |
| title_short | Characterization of Two Small Heat Shock Protein Genes (<i>Hsp17.4</i> and <i>Hs20.3</i>) from <i>Sitodiplosis mosellana</i>, and Their Expression Regulation during Diapause |
| title_sort | characterization of two small heat shock protein genes i hsp17 4 i and i hs20 3 i from i sitodiplosis mosellana i and their expression regulation during diapause |
| topic | <i>Sitodiplosis mosellana</i> <i>Hsp17.4</i> <i>Hsp20.3</i> diapause stress tolerance ecdysone |
| url | https://www.mdpi.com/2075-4450/12/2/119 |
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