Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms
Dwindling water sources increase the need for efficient wastewater treatment. Solar-driven algal turf scrubber (ATS) system may remediate wastewater by supporting the development and growth of periphytic microbiomes that function and interact in a highly dynamic manner through symbiotic interactions...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2994 |
| _version_ | 1827574049221378048 |
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| author | Olga Blifernez-Klassen Julia Hassa Diana L. Reinecke Tobias Busche Viktor Klassen Olaf Kruse |
| author_facet | Olga Blifernez-Klassen Julia Hassa Diana L. Reinecke Tobias Busche Viktor Klassen Olaf Kruse |
| author_sort | Olga Blifernez-Klassen |
| collection | DOAJ |
| description | Dwindling water sources increase the need for efficient wastewater treatment. Solar-driven algal turf scrubber (ATS) system may remediate wastewater by supporting the development and growth of periphytic microbiomes that function and interact in a highly dynamic manner through symbiotic interactions. Using ITS and 16S rRNA gene amplicon sequencing, we profiled the microbial communities of four microbial biofilms from ATS systems operated with municipal wastewater (mWW), diluted cattle and pig manure (CattleM and PigM), and biogas plant effluent supernatant (BGE) in comparison to the initial inocula and the respective wastewater substrates. The wastewater-driven biofilms differed significantly in their biodiversity and structure, exhibiting an inocula-independent but substrate-dependent establishment of the microbial communities. The prokaryotic communities were comparable among themselves and with other microbiomes of aquatic environments and were dominated by metabolically flexible prokaryotes such as nitrifiers, polyphosphate-accumulating and algicide-producing microorganisms, and anoxygenic photoautotrophs. Striking differences occurred in eukaryotic communities: While the mWW biofilm was characterized by high biodiversity and many filamentous (benthic) microalgae, the agricultural wastewater-fed biofilms consisted of less diverse communities with few benthic taxa mainly inhabited by unicellular chlorophytes and saprophytes/parasites. This study advances our understanding of the microbiome structure and function within the ATS-based wastewater treatment process. |
| first_indexed | 2024-03-08T20:31:12Z |
| format | Article |
| id | doaj.art-b16fd88863254e9a9882b215874dc8df |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:31:12Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b16fd88863254e9a9882b215874dc8df2023-12-22T14:26:19ZengMDPI AGMicroorganisms2076-26072023-12-011112299410.3390/microorganisms11122994Microbial Diversity and Community Structure of Wastewater-Driven Microalgal BiofilmsOlga Blifernez-Klassen0Julia Hassa1Diana L. Reinecke2Tobias Busche3Viktor Klassen4Olaf Kruse5Algae Biotechnology and Bioenergy, Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, GermanyCenter for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, GermanyInstitute of Bio- and Geosciences, Plant Sciences, Forschungszentrum Jülich, Wilhelm-Johnen-Strasse, 52428 Juelich, GermanyCenter for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, GermanyAlgae Biotechnology and Bioenergy, Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, GermanyAlgae Biotechnology and Bioenergy, Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, GermanyDwindling water sources increase the need for efficient wastewater treatment. Solar-driven algal turf scrubber (ATS) system may remediate wastewater by supporting the development and growth of periphytic microbiomes that function and interact in a highly dynamic manner through symbiotic interactions. Using ITS and 16S rRNA gene amplicon sequencing, we profiled the microbial communities of four microbial biofilms from ATS systems operated with municipal wastewater (mWW), diluted cattle and pig manure (CattleM and PigM), and biogas plant effluent supernatant (BGE) in comparison to the initial inocula and the respective wastewater substrates. The wastewater-driven biofilms differed significantly in their biodiversity and structure, exhibiting an inocula-independent but substrate-dependent establishment of the microbial communities. The prokaryotic communities were comparable among themselves and with other microbiomes of aquatic environments and were dominated by metabolically flexible prokaryotes such as nitrifiers, polyphosphate-accumulating and algicide-producing microorganisms, and anoxygenic photoautotrophs. Striking differences occurred in eukaryotic communities: While the mWW biofilm was characterized by high biodiversity and many filamentous (benthic) microalgae, the agricultural wastewater-fed biofilms consisted of less diverse communities with few benthic taxa mainly inhabited by unicellular chlorophytes and saprophytes/parasites. This study advances our understanding of the microbiome structure and function within the ATS-based wastewater treatment process.https://www.mdpi.com/2076-2607/11/12/2994environmental microbiome structurewastewatertaxonomic profilingbiofilmmicrobial biodiversitymicroalga–bacteria consortia |
| spellingShingle | Olga Blifernez-Klassen Julia Hassa Diana L. Reinecke Tobias Busche Viktor Klassen Olaf Kruse Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms Microorganisms environmental microbiome structure wastewater taxonomic profiling biofilm microbial biodiversity microalga–bacteria consortia |
| title | Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms |
| title_full | Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms |
| title_fullStr | Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms |
| title_full_unstemmed | Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms |
| title_short | Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms |
| title_sort | microbial diversity and community structure of wastewater driven microalgal biofilms |
| topic | environmental microbiome structure wastewater taxonomic profiling biofilm microbial biodiversity microalga–bacteria consortia |
| url | https://www.mdpi.com/2076-2607/11/12/2994 |
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