The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation
<i>LUX ARRHYTHMO</i> (<i>LUX</i>) plays a key role in circadian rhythms and flowering. Here, we identified the <i>MtLUX</i> gene which is the putative ortholog of <i>LUX</i> in <i>Medicago truncatula</i>. The roles of <i>MtLUX</i&g...
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/3473 |
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| author | Yiming Kong Yuxue Zhang Xiu Liu Zhe Meng Xiaolin Yu Chuanen Zhou Lu Han |
| author_facet | Yiming Kong Yuxue Zhang Xiu Liu Zhe Meng Xiaolin Yu Chuanen Zhou Lu Han |
| author_sort | Yiming Kong |
| collection | DOAJ |
| description | <i>LUX ARRHYTHMO</i> (<i>LUX</i>) plays a key role in circadian rhythms and flowering. Here, we identified the <i>MtLUX</i> gene which is the putative ortholog of <i>LUX</i> in <i>Medicago truncatula</i>. The roles of <i>MtLUX</i>, in both the nodulation belowground and leaf movement aboveground, were investigated by characterizing a loss-of-function <i>mtlux</i> mutant. <i>MtLUX</i> was required for the control of flowering time under both long-day and short-day conditions. Further investigations showed that the early flowering in the <i>mtlux</i> mutant was correlated with the elevated expression level of the <i>MtFTa1</i> gene but in a <i>CO</i>-<i>like</i> independent manner. <i>MtLUX</i> played a conserved role in the regulatory interactions with <i>MtLHY</i>, <i>MtTOC1</i>, and <i>MtPRR</i> genes, which is similar to those in other species. Meanwhile, the unexpected functions of <i>MtLUX</i> were revealed in nodule formation and nyctinastic leaf movement, probably through the indirect regulation in <i>MtLHY</i>. Its participation in nodulation is of interest in the context of functional conservation and the neo-functionalization of the products of <i>LUX</i> orthologs. |
| first_indexed | 2024-03-09T11:48:42Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:48:42Z |
| publishDate | 2022-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b1719653dd1e408aab535c8bd0cf83272023-11-30T23:17:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01237347310.3390/ijms23073473The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and NodulationYiming Kong0Yuxue Zhang1Xiu Liu2Zhe Meng3Xiaolin Yu4Chuanen Zhou5Lu Han6The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Science, Shandong Normal University, Ji’nan 250300, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, China<i>LUX ARRHYTHMO</i> (<i>LUX</i>) plays a key role in circadian rhythms and flowering. Here, we identified the <i>MtLUX</i> gene which is the putative ortholog of <i>LUX</i> in <i>Medicago truncatula</i>. The roles of <i>MtLUX</i>, in both the nodulation belowground and leaf movement aboveground, were investigated by characterizing a loss-of-function <i>mtlux</i> mutant. <i>MtLUX</i> was required for the control of flowering time under both long-day and short-day conditions. Further investigations showed that the early flowering in the <i>mtlux</i> mutant was correlated with the elevated expression level of the <i>MtFTa1</i> gene but in a <i>CO</i>-<i>like</i> independent manner. <i>MtLUX</i> played a conserved role in the regulatory interactions with <i>MtLHY</i>, <i>MtTOC1</i>, and <i>MtPRR</i> genes, which is similar to those in other species. Meanwhile, the unexpected functions of <i>MtLUX</i> were revealed in nodule formation and nyctinastic leaf movement, probably through the indirect regulation in <i>MtLHY</i>. Its participation in nodulation is of interest in the context of functional conservation and the neo-functionalization of the products of <i>LUX</i> orthologs.https://www.mdpi.com/1422-0067/23/7/3473legume<i>Medicago truncatula</i><i>MtLUX</i><i>MtFTa1</i>circadian clockphotoperiodic flowering |
| spellingShingle | Yiming Kong Yuxue Zhang Xiu Liu Zhe Meng Xiaolin Yu Chuanen Zhou Lu Han The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation International Journal of Molecular Sciences legume <i>Medicago truncatula</i> <i>MtLUX</i> <i>MtFTa1</i> circadian clock photoperiodic flowering |
| title | The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation |
| title_full | The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation |
| title_fullStr | The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation |
| title_full_unstemmed | The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation |
| title_short | The Conserved and Specific Roles of the <i>LUX ARRHYTHMO</i> in Circadian Clock and Nodulation |
| title_sort | conserved and specific roles of the i lux arrhythmo i in circadian clock and nodulation |
| topic | legume <i>Medicago truncatula</i> <i>MtLUX</i> <i>MtFTa1</i> circadian clock photoperiodic flowering |
| url | https://www.mdpi.com/1422-0067/23/7/3473 |
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