Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L.
Due to the increasing use of <i>Physalis alkekengi</i> L. as a food supplement and starting material for tea preparation, a comprehensive analysis of green extracts was performed. Two different extraction methods were applied to yellow <i>Physalis alkekengi</i> L. fruit and c...
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MDPI AG
2023-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/12/2101 |
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| author | Maria Assunta Crescenzi Gabriele Serreli Monica Deiana Carlo I. G. Tuberoso Paola Montoro Sonia Piacente |
| author_facet | Maria Assunta Crescenzi Gabriele Serreli Monica Deiana Carlo I. G. Tuberoso Paola Montoro Sonia Piacente |
| author_sort | Maria Assunta Crescenzi |
| collection | DOAJ |
| description | Due to the increasing use of <i>Physalis alkekengi</i> L. as a food supplement and starting material for tea preparation, a comprehensive analysis of green extracts was performed. Two different extraction methods were applied to yellow <i>Physalis alkekengi</i> L. fruit and calyx and compared: hydroalcoholic extraction and decoction. Characterization of the metabolome of the calyx and fruit of yellow <i>Physalis alkekengi</i> L. was performed by LC-ESI/LTQOrbitrap/MS followed by LC-ESI/LTQOrbitrap/MS/MS to identify 58 phytocompounds using the two different extraction techniques. Subsequently, through preliminary spectrophotometric assays followed by cell studies, the antioxidant activity of the different <i>Physalis alkekengi</i> L. extracts were evaluated. It was found that <i>Physalis alkekengi</i> L. extracts are a good source of metabolites such as flavonoids, organic acids, phenylpropanoids, physalins and carotenoids, with various biological activities, in particular, antioxidant activity capable of reducing the production of free radicals in intestinal Caco-2 cells. For the first time, an integrated approach (metabolomics approach and antioxidant evaluation) was applied to the study of <i>Physalis alkekengi</i> green extracts and decoctions, the green extraction method mostly used in herbal preparations. An interesting finding was the high antioxidant activity shown by these extracts. |
| first_indexed | 2024-03-08T21:02:51Z |
| format | Article |
| id | doaj.art-b175ff8dfc7c4eb8abdf6babdd8d5c25 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T21:02:51Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b175ff8dfc7c4eb8abdf6babdd8d5c252023-12-22T13:48:32ZengMDPI AGAntioxidants2076-39212023-12-011212210110.3390/antiox12122101Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L.Maria Assunta Crescenzi0Gabriele Serreli1Monica Deiana2Carlo I. G. Tuberoso3Paola Montoro4Sonia Piacente5Department of Pharmacy, University of the Study of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, ItalyUnit of Experimental Pathology, Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria SS 554, 09042 Monserrato, ItalyUnit of Experimental Pathology, Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria SS 554, 09042 Monserrato, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, ItalyDepartment of Pharmacy, University of the Study of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, ItalyDepartment of Pharmacy, University of the Study of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, ItalyDue to the increasing use of <i>Physalis alkekengi</i> L. as a food supplement and starting material for tea preparation, a comprehensive analysis of green extracts was performed. Two different extraction methods were applied to yellow <i>Physalis alkekengi</i> L. fruit and calyx and compared: hydroalcoholic extraction and decoction. Characterization of the metabolome of the calyx and fruit of yellow <i>Physalis alkekengi</i> L. was performed by LC-ESI/LTQOrbitrap/MS followed by LC-ESI/LTQOrbitrap/MS/MS to identify 58 phytocompounds using the two different extraction techniques. Subsequently, through preliminary spectrophotometric assays followed by cell studies, the antioxidant activity of the different <i>Physalis alkekengi</i> L. extracts were evaluated. It was found that <i>Physalis alkekengi</i> L. extracts are a good source of metabolites such as flavonoids, organic acids, phenylpropanoids, physalins and carotenoids, with various biological activities, in particular, antioxidant activity capable of reducing the production of free radicals in intestinal Caco-2 cells. For the first time, an integrated approach (metabolomics approach and antioxidant evaluation) was applied to the study of <i>Physalis alkekengi</i> green extracts and decoctions, the green extraction method mostly used in herbal preparations. An interesting finding was the high antioxidant activity shown by these extracts.https://www.mdpi.com/2076-3921/12/12/2101LC-ESI/LTQOrbitrap MS<i>Physalis alkekengi</i> L.antiradical scavenging activitymetabolic profiling |
| spellingShingle | Maria Assunta Crescenzi Gabriele Serreli Monica Deiana Carlo I. G. Tuberoso Paola Montoro Sonia Piacente Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L. Antioxidants LC-ESI/LTQOrbitrap MS <i>Physalis alkekengi</i> L. antiradical scavenging activity metabolic profiling |
| title | Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L. |
| title_full | Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L. |
| title_fullStr | Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L. |
| title_full_unstemmed | Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L. |
| title_short | Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from <i>Physalis alkekengi</i> L. |
| title_sort | metabolite profiling through lc esi ltqorbitrap ms analysis of antioxidant extracts from i physalis alkekengi i l |
| topic | LC-ESI/LTQOrbitrap MS <i>Physalis alkekengi</i> L. antiradical scavenging activity metabolic profiling |
| url | https://www.mdpi.com/2076-3921/12/12/2101 |
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