PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil
Polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment res...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.952374/full |
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| author | Monika Sandhu Atish T. Paul Jarosław Proćków José Manuel Pérez de la Lastra Prabhat N. Jha |
| author_facet | Monika Sandhu Atish T. Paul Jarosław Proćków José Manuel Pérez de la Lastra Prabhat N. Jha |
| author_sort | Monika Sandhu |
| collection | DOAJ |
| description | Polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment resulted in the isolation of 33 strains of PCB-contaminated soil nearby Bhilai steel plant, Chhattisgarh, India. Based on the prominent growth using biphenyl as the sole carbon source and the confirmation of its degradation by GC-MS/MS analysis, four isolates were selected for further study. The isolates identified by 16S rRNA gene sequencing were Pseudomonas aeruginosa MAPB-2, Pseudomonas plecoglossicida MAPB-6, Brucella anthropi MAPB-9, and Priestia megaterium MAPB-27. The isolate MAPB-9 showed a degradation of 66.15% biphenyl, while MAPB-2, MAPB-6, and MAPB-27 showed a degradation of 62.06, 57.02, and 56.55%, respectively in 48 h. Additionally, the degradation ability of these strains was enhanced with addition of co-metabolite glucose (0.2%) in the culture medium. Addition of glucose showed 100% degradation of biphenyl by MAPB-9, in 48 h, while MAPB-6, MAPB-2, and MAPB-27 showed 97.1, 67.5, and 53.3% degradation, respectively as analyzed by GC-MS/MS. Furthermore, in the presence of inducer, PCB-77 was found to be 59.89, 30.49, 27.19, and 4.43% degraded by MAPB-6, MAPB-9, MAPB-2, and MAPB-27, respectively in 7 d. The production of biosurfactants that aid in biodegradation process were observed in all the isolates. This was confirmed by ATR-FTIR analysis that showed the presence of major functional groups (CH2, CH3, CH, = CH2, C–O–C, C-O) of the biosurfactant. The biosurfactants were further identified by HPTLC and GC-MS/MS analysis. Present study is the first to report PCB-77 degradation potential of Pseudomonas aeruginosa, B. anthropi, Pseudomonas plecoglossicida, and Priestia megaterium. Similarly, this is the first report on Pseudomonas plecoglossicida and Priestia megaterium for PCB biodegradation. Our results suggest that the above isolates can be used for the biodegradation of biphenyl and PCB-77 in PCB-contaminated soil. |
| first_indexed | 2024-04-12T18:19:00Z |
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| language | English |
| last_indexed | 2024-04-12T18:19:00Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-b17846cf23004177a6855f71f77bd0282022-12-22T03:21:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.952374952374PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soilMonika Sandhu0Atish T. Paul1Jarosław Proćków2José Manuel Pérez de la Lastra3Prabhat N. Jha4Department of Biological Sciences, Birla Institute of Technology and Science Pilani, Pilani, Rajasthan, IndiaDepartment of Pharmacy, Birla Institute of Technology and Science Pilani, Pilani, Rajasthan, IndiaDepartment of Plant Biology, Institute of Environmental Biology, Wrocław University of Environmental and Life Sciences, Wrocław, PolandBiotecnología de Macromoléculas, Instituto de Productos Naturales y Agrobiología (IPNA-CSIC), San Cristóbal de la Laguna, SpainDepartment of Biological Sciences, Birla Institute of Technology and Science Pilani, Pilani, Rajasthan, IndiaPolychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment resulted in the isolation of 33 strains of PCB-contaminated soil nearby Bhilai steel plant, Chhattisgarh, India. Based on the prominent growth using biphenyl as the sole carbon source and the confirmation of its degradation by GC-MS/MS analysis, four isolates were selected for further study. The isolates identified by 16S rRNA gene sequencing were Pseudomonas aeruginosa MAPB-2, Pseudomonas plecoglossicida MAPB-6, Brucella anthropi MAPB-9, and Priestia megaterium MAPB-27. The isolate MAPB-9 showed a degradation of 66.15% biphenyl, while MAPB-2, MAPB-6, and MAPB-27 showed a degradation of 62.06, 57.02, and 56.55%, respectively in 48 h. Additionally, the degradation ability of these strains was enhanced with addition of co-metabolite glucose (0.2%) in the culture medium. Addition of glucose showed 100% degradation of biphenyl by MAPB-9, in 48 h, while MAPB-6, MAPB-2, and MAPB-27 showed 97.1, 67.5, and 53.3% degradation, respectively as analyzed by GC-MS/MS. Furthermore, in the presence of inducer, PCB-77 was found to be 59.89, 30.49, 27.19, and 4.43% degraded by MAPB-6, MAPB-9, MAPB-2, and MAPB-27, respectively in 7 d. The production of biosurfactants that aid in biodegradation process were observed in all the isolates. This was confirmed by ATR-FTIR analysis that showed the presence of major functional groups (CH2, CH3, CH, = CH2, C–O–C, C-O) of the biosurfactant. The biosurfactants were further identified by HPTLC and GC-MS/MS analysis. Present study is the first to report PCB-77 degradation potential of Pseudomonas aeruginosa, B. anthropi, Pseudomonas plecoglossicida, and Priestia megaterium. Similarly, this is the first report on Pseudomonas plecoglossicida and Priestia megaterium for PCB biodegradation. Our results suggest that the above isolates can be used for the biodegradation of biphenyl and PCB-77 in PCB-contaminated soil.https://www.frontiersin.org/articles/10.3389/fmicb.2022.952374/fullpolychlorinated biphenylbiodegradationbiosurfactantPseudomonas aeruginosaPseudomonas plecoglossicidaPriestia megaterium |
| spellingShingle | Monika Sandhu Atish T. Paul Jarosław Proćków José Manuel Pérez de la Lastra Prabhat N. Jha PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil Frontiers in Microbiology polychlorinated biphenyl biodegradation biosurfactant Pseudomonas aeruginosa Pseudomonas plecoglossicida Priestia megaterium |
| title | PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil |
| title_full | PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil |
| title_fullStr | PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil |
| title_full_unstemmed | PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil |
| title_short | PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil |
| title_sort | pcb 77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil |
| topic | polychlorinated biphenyl biodegradation biosurfactant Pseudomonas aeruginosa Pseudomonas plecoglossicida Priestia megaterium |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.952374/full |
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