Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell
In this article, we describe an enzyme-based, membraneless, microfluidic biofuel cell for the continuous determination of glucose using electrochemical power generation as a transducing signal. Enzymes were immobilized on multi-walled carbon nanotube (MWCNT) electrodes placed parallel to the co-lami...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/12/1129 |
| _version_ | 1797544090327318528 |
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| author | Haroon Khan Jin Ho Choi Asad Ullah Young Ho Kim Gyu Man Kim |
| author_facet | Haroon Khan Jin Ho Choi Asad Ullah Young Ho Kim Gyu Man Kim |
| author_sort | Haroon Khan |
| collection | DOAJ |
| description | In this article, we describe an enzyme-based, membraneless, microfluidic biofuel cell for the continuous determination of glucose using electrochemical power generation as a transducing signal. Enzymes were immobilized on multi-walled carbon nanotube (MWCNT) electrodes placed parallel to the co-laminar flow in a Y-shaped microchannel. The microchannel was produced with polydimethylsiloxane (PDMS) using soft lithography, while the MWCNT electrodes were replicated via a PDMS stencil on indium tin oxide (ITO) glass. Moreover, the electrodes were modified with glucose oxidase and laccase by direct covalent bonding. The device was studied at different MWCNT deposition amounts and electrolyte flow rates to achieve optimum settings. The experimental results demonstrated that glucose could be determined linearly up to a concentration of 4 mM at a sensitivity of 31 mV∙mM<sup>−1</sup>cm<sup>−2</sup>. |
| first_indexed | 2024-03-10T13:54:30Z |
| format | Article |
| id | doaj.art-b17de35e997747cebb7dfb01d288aaeb |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T13:54:30Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-b17de35e997747cebb7dfb01d288aaeb2023-11-21T01:46:26ZengMDPI AGMicromachines2072-666X2020-12-011112112910.3390/mi11121129Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel CellHaroon Khan0Jin Ho Choi1Asad Ullah2Young Ho Kim3Gyu Man Kim4School of Mechanical Engineering, Kyungpook National University, Daegu 41566, KoreaInsung Medical Co., Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41071, KoreaSchool of Mechanical Engineering, Kyungpook National University, Daegu 41566, KoreaMedical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, KoreaSchool of Mechanical Engineering, Kyungpook National University, Daegu 41566, KoreaIn this article, we describe an enzyme-based, membraneless, microfluidic biofuel cell for the continuous determination of glucose using electrochemical power generation as a transducing signal. Enzymes were immobilized on multi-walled carbon nanotube (MWCNT) electrodes placed parallel to the co-laminar flow in a Y-shaped microchannel. The microchannel was produced with polydimethylsiloxane (PDMS) using soft lithography, while the MWCNT electrodes were replicated via a PDMS stencil on indium tin oxide (ITO) glass. Moreover, the electrodes were modified with glucose oxidase and laccase by direct covalent bonding. The device was studied at different MWCNT deposition amounts and electrolyte flow rates to achieve optimum settings. The experimental results demonstrated that glucose could be determined linearly up to a concentration of 4 mM at a sensitivity of 31 mV∙mM<sup>−1</sup>cm<sup>−2</sup>.https://www.mdpi.com/2072-666X/11/12/1129continuous glucose determinationmembranelessenzymatic biofuel cell |
| spellingShingle | Haroon Khan Jin Ho Choi Asad Ullah Young Ho Kim Gyu Man Kim Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell Micromachines continuous glucose determination membraneless enzymatic biofuel cell |
| title | Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell |
| title_full | Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell |
| title_fullStr | Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell |
| title_full_unstemmed | Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell |
| title_short | Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell |
| title_sort | continuous determination of glucose using a membraneless microfluidic enzymatic biofuel cell |
| topic | continuous glucose determination membraneless enzymatic biofuel cell |
| url | https://www.mdpi.com/2072-666X/11/12/1129 |
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