Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability
<p>Abstract</p> <p>Background</p> <p>Elucidating the molecular mechanisms by which tumors become resistant to Herceptin is critical for the treatment of Her2-overexpressed metastatic breast cancer.</p> <p>Methods</p> <p>To further understand Herc...
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| Format: | Article |
| Language: | English |
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BMC
2008-10-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/8/286 |
| _version_ | 1828486525329866752 |
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| author | Kong Sun-Young Lee Ho-Young Kim Seok-Ki Kwon Bumi Kim Kyung-Hee Kang Keon Kang Se Lee Eun Jang Sang-Geun Yoo Byong |
| author_facet | Kong Sun-Young Lee Ho-Young Kim Seok-Ki Kwon Bumi Kim Kyung-Hee Kang Keon Kang Se Lee Eun Jang Sang-Geun Yoo Byong |
| author_sort | Kong Sun-Young |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Elucidating the molecular mechanisms by which tumors become resistant to Herceptin is critical for the treatment of Her2-overexpressed metastatic breast cancer.</p> <p>Methods</p> <p>To further understand Herceptin resistance mechanisms at the molecular level, we used comparative proteome approaches to analyze two human breast cancer cell lines; Her2-positive SK-BR-3 cells and its Herceptin-resistant SK-BR-3 (SK-BR-3 HR) cells.</p> <p>Results</p> <p>Heat-shock protein 27 (HSP27) expression was shown to be upregulated in SK-BR-3 HR cells. Suppression of HSP27 by specific siRNA transfection increased the susceptibility of SK-BR-3 HR cells to Herceptin. In the presence of Herceptin, Her2 was downregulated in both cell lines. However, Her2 expression was reduced by a greater amount in SK-BR-3 parent cells than in SK-BR-3 HR cells. Interestingly, co-immunoprecipitation analysis showed that HSP27 can bind to Her2. In the absence of Herceptin, HSP27 expression is suppressed and Her2 expression is reduced, indicating that downregulation of Her2 by Herceptin can be obstructed by the formation of a Her2-HSP27 complex.</p> <p>Conclusion</p> <p>Our present study demonstrates that upregulated HSP27 in human breast cancer cells can reduce Herceptin susceptibility by increasing Her2 protein stability.</p> |
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| format | Article |
| id | doaj.art-b17df97ef20743ffb712b90578f076db |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T09:34:25Z |
| publishDate | 2008-10-01 |
| publisher | BMC |
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| series | BMC Cancer |
| spelling | doaj.art-b17df97ef20743ffb712b90578f076db2022-12-22T01:12:56ZengBMCBMC Cancer1471-24072008-10-018128610.1186/1471-2407-8-286Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stabilityKong Sun-YoungLee Ho-YoungKim Seok-KiKwon BumiKim Kyung-HeeKang KeonKang SeLee EunJang Sang-GeunYoo Byong<p>Abstract</p> <p>Background</p> <p>Elucidating the molecular mechanisms by which tumors become resistant to Herceptin is critical for the treatment of Her2-overexpressed metastatic breast cancer.</p> <p>Methods</p> <p>To further understand Herceptin resistance mechanisms at the molecular level, we used comparative proteome approaches to analyze two human breast cancer cell lines; Her2-positive SK-BR-3 cells and its Herceptin-resistant SK-BR-3 (SK-BR-3 HR) cells.</p> <p>Results</p> <p>Heat-shock protein 27 (HSP27) expression was shown to be upregulated in SK-BR-3 HR cells. Suppression of HSP27 by specific siRNA transfection increased the susceptibility of SK-BR-3 HR cells to Herceptin. In the presence of Herceptin, Her2 was downregulated in both cell lines. However, Her2 expression was reduced by a greater amount in SK-BR-3 parent cells than in SK-BR-3 HR cells. Interestingly, co-immunoprecipitation analysis showed that HSP27 can bind to Her2. In the absence of Herceptin, HSP27 expression is suppressed and Her2 expression is reduced, indicating that downregulation of Her2 by Herceptin can be obstructed by the formation of a Her2-HSP27 complex.</p> <p>Conclusion</p> <p>Our present study demonstrates that upregulated HSP27 in human breast cancer cells can reduce Herceptin susceptibility by increasing Her2 protein stability.</p>http://www.biomedcentral.com/1471-2407/8/286 |
| spellingShingle | Kong Sun-Young Lee Ho-Young Kim Seok-Ki Kwon Bumi Kim Kyung-Hee Kang Keon Kang Se Lee Eun Jang Sang-Geun Yoo Byong Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability BMC Cancer |
| title | Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability |
| title_full | Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability |
| title_fullStr | Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability |
| title_full_unstemmed | Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability |
| title_short | Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability |
| title_sort | upregulated hsp27 in human breast cancer cells reduces herceptin susceptibility by increasing her2 protein stability |
| url | http://www.biomedcentral.com/1471-2407/8/286 |
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