Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae)
According to the recent taxonomic and phylogenetic revision of the family Hylidae, species of the former Scinax catharinae (Boulenger, 1888) clade were included in the resurrected genus Ololygon Fitzinger, 1843, while species of the Scinax ruber (Laurenti, 1768) clade were mostly included in the gen...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2017-04-01
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| Series: | Comparative Cytogenetics |
| Online Access: | http://compcytogen.pensoft.net/articles.php?id=11254 |
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| author | Simone Lilian Gruber Gabriela Isabela Gomes de Oliveira Ana Paula Zampieri Silva Hideki Narimatsu Célio Fernando Baptista Haddad Sanae Kasahara |
| author_facet | Simone Lilian Gruber Gabriela Isabela Gomes de Oliveira Ana Paula Zampieri Silva Hideki Narimatsu Célio Fernando Baptista Haddad Sanae Kasahara |
| author_sort | Simone Lilian Gruber |
| collection | DOAJ |
| description | According to the recent taxonomic and phylogenetic revision of the family Hylidae, species of the former Scinax catharinae (Boulenger, 1888) clade were included in the resurrected genus Ololygon Fitzinger, 1843, while species of the Scinax ruber (Laurenti, 1768) clade were mostly included in the genus Scinax Wagler, 1830, and two were allocated to the newly created genus Julianus Duellman et al., 2016. Although all the species of the former Scinax genus shared a diploid number of 2n = 24 and the same fundamental number of chromosome arms of FN = 48, two karyotypic constitutions were unequivocally recognized, related mainly to the distinct size and morphology of the first two chromosome pairs. Some possible mechanisms for these differences had been suggested, but without any experimental evidence. In this paper, a comparison was carried out based on replication chromosome banding, obtained after DNA incorporation of 5-bromodeoxiuridine in chromosomes of Ololygon and Scinax. The obtained results revealed that the loss of repetitive segments in chromosome pairs 1 and 2 was the mechanism responsible for karyotype difference. The distinct localization of the nucleolus organizer regions in the species of both genera also differentiates the two karyotypic constitutions. |
| first_indexed | 2024-12-11T11:03:55Z |
| format | Article |
| id | doaj.art-b17ee55e2e98474bb7aa771ec3dba26c |
| institution | Directory Open Access Journal |
| issn | 1993-0771 1993-078X |
| language | English |
| last_indexed | 2024-12-11T11:03:55Z |
| publishDate | 2017-04-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Comparative Cytogenetics |
| spelling | doaj.art-b17ee55e2e98474bb7aa771ec3dba26c2022-12-22T01:09:47ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2017-04-0111226728310.3897/CompCytogen.v11i2.1125411254Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae)Simone Lilian Gruber0Gabriela Isabela Gomes de Oliveira1Ana Paula Zampieri Silva2Hideki Narimatsu3Célio Fernando Baptista Haddad4Sanae Kasahara5UNESP, Universidade Estadual PaulistaUNESP, Universidade Estadual PaulistaUNESP, Universidade Estadual PaulistaUNESP, Universidade Estadual PaulistaUNESP, Universidade Estadual PaulistaUNESP, Universidade Estadual PaulistaAccording to the recent taxonomic and phylogenetic revision of the family Hylidae, species of the former Scinax catharinae (Boulenger, 1888) clade were included in the resurrected genus Ololygon Fitzinger, 1843, while species of the Scinax ruber (Laurenti, 1768) clade were mostly included in the genus Scinax Wagler, 1830, and two were allocated to the newly created genus Julianus Duellman et al., 2016. Although all the species of the former Scinax genus shared a diploid number of 2n = 24 and the same fundamental number of chromosome arms of FN = 48, two karyotypic constitutions were unequivocally recognized, related mainly to the distinct size and morphology of the first two chromosome pairs. Some possible mechanisms for these differences had been suggested, but without any experimental evidence. In this paper, a comparison was carried out based on replication chromosome banding, obtained after DNA incorporation of 5-bromodeoxiuridine in chromosomes of Ololygon and Scinax. The obtained results revealed that the loss of repetitive segments in chromosome pairs 1 and 2 was the mechanism responsible for karyotype difference. The distinct localization of the nucleolus organizer regions in the species of both genera also differentiates the two karyotypic constitutions.http://compcytogen.pensoft.net/articles.php?id=11254 |
| spellingShingle | Simone Lilian Gruber Gabriela Isabela Gomes de Oliveira Ana Paula Zampieri Silva Hideki Narimatsu Célio Fernando Baptista Haddad Sanae Kasahara Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae) Comparative Cytogenetics |
| title | Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae) |
| title_full | Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae) |
| title_fullStr | Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae) |
| title_full_unstemmed | Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae) |
| title_short | Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae) |
| title_sort | comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs ololygon and scinax arboranae hylidae scinaxinae |
| url | http://compcytogen.pensoft.net/articles.php?id=11254 |
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