Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells
The effect of docosahexaenoic acid (DHA) on cyclooxygenase expression induced by interleukin (IL)-1β and phorbol 12-myristate 13-acetate (PMA) in rat vascular smooth muscle cells (VSMCs) was investigated in order to clarify the cellular mechanism of cardiovascular protective effects. DHA and eicosap...
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| Format: | Article |
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Elsevier
2005-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319321413 |
| _version_ | 1830406900341014528 |
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| author | Takuji Machida Miho Hiramatsu Naoya Hamaue Masaru Minami Masahiko Hirafuji |
| author_facet | Takuji Machida Miho Hiramatsu Naoya Hamaue Masaru Minami Masahiko Hirafuji |
| author_sort | Takuji Machida |
| collection | DOAJ |
| description | The effect of docosahexaenoic acid (DHA) on cyclooxygenase expression induced by interleukin (IL)-1β and phorbol 12-myristate 13-acetate (PMA) in rat vascular smooth muscle cells (VSMCs) was investigated in order to clarify the cellular mechanism of cardiovascular protective effects. DHA and eicosapentaenoic acid slightly enhanced IL-1β-induced cyclooxygenase (COX)-2, but not COX-1, expression, whereas arachidonic acid had no effect. DHA also slightly enhanced PMA-induced COX-2 expression. DHA stimulated both rapid and prolonged activation of p44/42, but not p38, mitogen-activated protein kinase (MAPK) induced by IL-1β and PMA. These results suggest that DHA enhances the COX-2 expression by selectively facilitating p44/42 MAPK activation in VSMCs. Keywords:: docosahexaenoic acid, cyclooxygenase-2, vascular smooth muscle cell |
| first_indexed | 2024-12-20T18:18:03Z |
| format | Article |
| id | doaj.art-b17f0b80f8e744d7962f2feb8bef1de2 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-20T18:18:03Z |
| publishDate | 2005-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-b17f0b80f8e744d7962f2feb8bef1de22022-12-21T19:30:19ZengElsevierJournal of Pharmacological Sciences1347-86132005-01-01991113116Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle CellsTakuji Machida0Miho Hiramatsu1Naoya Hamaue2Masaru Minami3Masahiko Hirafuji4Department of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan; Corresponding author. FAX: +81-0133-23-1370 E-mail: hirafuji@hoku-iryo-u.ac.jpThe effect of docosahexaenoic acid (DHA) on cyclooxygenase expression induced by interleukin (IL)-1β and phorbol 12-myristate 13-acetate (PMA) in rat vascular smooth muscle cells (VSMCs) was investigated in order to clarify the cellular mechanism of cardiovascular protective effects. DHA and eicosapentaenoic acid slightly enhanced IL-1β-induced cyclooxygenase (COX)-2, but not COX-1, expression, whereas arachidonic acid had no effect. DHA also slightly enhanced PMA-induced COX-2 expression. DHA stimulated both rapid and prolonged activation of p44/42, but not p38, mitogen-activated protein kinase (MAPK) induced by IL-1β and PMA. These results suggest that DHA enhances the COX-2 expression by selectively facilitating p44/42 MAPK activation in VSMCs. Keywords:: docosahexaenoic acid, cyclooxygenase-2, vascular smooth muscle cellhttp://www.sciencedirect.com/science/article/pii/S1347861319321413 |
| spellingShingle | Takuji Machida Miho Hiramatsu Naoya Hamaue Masaru Minami Masahiko Hirafuji Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells Journal of Pharmacological Sciences |
| title | Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells |
| title_full | Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells |
| title_fullStr | Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells |
| title_full_unstemmed | Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells |
| title_short | Docosahexaenoic Acid Enhances Cyclooxygenase-2 Induction by Facilitating p44/42, but Not p38, Mitogen-Activated Protein Kinase Activation in Rat Vascular Smooth Muscle Cells |
| title_sort | docosahexaenoic acid enhances cyclooxygenase 2 induction by facilitating p44 42 but not p38 mitogen activated protein kinase activation in rat vascular smooth muscle cells |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319321413 |
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