Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity
Flavonoids constitute a group of polyphenolic compounds characterized by a common gamma-benzo- pyrone structure considered in numerous biological systems to possess antioxidant capacity. Among the different applications of flavonoids, its genoprotective capacity against damage induced by ionizing ra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/1/94 |
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| author | Miguel Alcaraz Amparo Olivares Daniel Gyingiri Achel José Antonio García-Gamuz Julián Castillo Miguel Alcaraz-Saura |
| author_facet | Miguel Alcaraz Amparo Olivares Daniel Gyingiri Achel José Antonio García-Gamuz Julián Castillo Miguel Alcaraz-Saura |
| author_sort | Miguel Alcaraz |
| collection | DOAJ |
| description | Flavonoids constitute a group of polyphenolic compounds characterized by a common gamma-benzo- pyrone structure considered in numerous biological systems to possess antioxidant capacity. Among the different applications of flavonoids, its genoprotective capacity against damage induced by ionizing radiation stands out, which has been related to antioxidant activity and its chemical structure. In this study, we determined the frequency of appearance of micronucleus in vivo by means of the micronucleus assay. This was conducted in mice treated with different flavonoids before and after exposure to 470 mGy X-rays; thereafter, their bone marrow polychromatophilic erythrocytes were evaluated to establish the structural factors enhancing the observed genoprotective effect. Our results in vivo show that the presence of a monomeric flavan-3-ol type structure, with absence of carbonyl group in position C4 of ring C, absence of conjugation between the carbons bearing the C2 = C3 double bond and the said ring, presence of a catechol group in ring B and characteristic hydroxylation in positions 5 and 7 of ring A are the structural characteristics that determine the highest degree of genoprotection. Additionally, a certain degree of polymerization of this flavonoid monomer, but maintaining significant levels of monomers and dimers, contributes to increasing the degree of genoprotection in the animals studied at both times of their administration (before and after exposure to X-rays). |
| first_indexed | 2024-03-10T03:01:03Z |
| format | Article |
| id | doaj.art-b18038de572845aea00e4c298f537575 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:01:03Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b18038de572845aea00e4c298f5375752023-11-23T12:47:15ZengMDPI AGAntioxidants2076-39212021-12-011119410.3390/antiox11010094Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and ActivityMiguel Alcaraz0Amparo Olivares1Daniel Gyingiri Achel2José Antonio García-Gamuz3Julián Castillo4Miguel Alcaraz-Saura5Radiology and Physical Medicine Department, School of Medicine, University of Murcia, 30100 Murcia, SpainRadiology and Physical Medicine Department, School of Medicine, University of Murcia, 30100 Murcia, SpainApplied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Accra GE-257-0465, GhanaRadiology and Physical Medicine Department, School of Medicine, University of Murcia, 30100 Murcia, SpainR&D Department, Iff Murcia Natural Ingredients, Site Plant: Nutrafur, Camino Viejo de Pliego, Km. 2, Box 182, 30820 Alcantarilla, SpainRadiology and Physical Medicine Department, School of Medicine, University of Murcia, 30100 Murcia, SpainFlavonoids constitute a group of polyphenolic compounds characterized by a common gamma-benzo- pyrone structure considered in numerous biological systems to possess antioxidant capacity. Among the different applications of flavonoids, its genoprotective capacity against damage induced by ionizing radiation stands out, which has been related to antioxidant activity and its chemical structure. In this study, we determined the frequency of appearance of micronucleus in vivo by means of the micronucleus assay. This was conducted in mice treated with different flavonoids before and after exposure to 470 mGy X-rays; thereafter, their bone marrow polychromatophilic erythrocytes were evaluated to establish the structural factors enhancing the observed genoprotective effect. Our results in vivo show that the presence of a monomeric flavan-3-ol type structure, with absence of carbonyl group in position C4 of ring C, absence of conjugation between the carbons bearing the C2 = C3 double bond and the said ring, presence of a catechol group in ring B and characteristic hydroxylation in positions 5 and 7 of ring A are the structural characteristics that determine the highest degree of genoprotection. Additionally, a certain degree of polymerization of this flavonoid monomer, but maintaining significant levels of monomers and dimers, contributes to increasing the degree of genoprotection in the animals studied at both times of their administration (before and after exposure to X-rays).https://www.mdpi.com/2076-3921/11/1/94radiation effectsradioprotectorsmicronucleusflavonoids |
| spellingShingle | Miguel Alcaraz Amparo Olivares Daniel Gyingiri Achel José Antonio García-Gamuz Julián Castillo Miguel Alcaraz-Saura Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity Antioxidants radiation effects radioprotectors micronucleus flavonoids |
| title | Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity |
| title_full | Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity |
| title_fullStr | Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity |
| title_full_unstemmed | Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity |
| title_short | Genoprotective Effect of Some Flavonoids against Genotoxic Damage Induced by X-rays In Vivo: Relationship between Structure and Activity |
| title_sort | genoprotective effect of some flavonoids against genotoxic damage induced by x rays in vivo relationship between structure and activity |
| topic | radiation effects radioprotectors micronucleus flavonoids |
| url | https://www.mdpi.com/2076-3921/11/1/94 |
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