Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications
<i>Rhodotorula</i> yeasts which are known as carotenogenic yeasts have a great industrial value due to their ability to produce carotenoids. In particular, the isolated yeast <i>Rhodotorula</i> sp. (strain ATL72) has been reported to be a promising producer of high concentrat...
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MDPI AG
2022-02-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/2/160 |
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| author | Amira Dyaa Hoda Soliman Ahmed Abdelrazak Bassem N. Samra Ebtihal Khojah Atef F. Ahmed Mohamed A. El-Esawi Ashraf Elsayed |
| author_facet | Amira Dyaa Hoda Soliman Ahmed Abdelrazak Bassem N. Samra Ebtihal Khojah Atef F. Ahmed Mohamed A. El-Esawi Ashraf Elsayed |
| author_sort | Amira Dyaa |
| collection | DOAJ |
| description | <i>Rhodotorula</i> yeasts which are known as carotenogenic yeasts have a great industrial value due to their ability to produce carotenoids. In particular, the isolated yeast <i>Rhodotorula</i> sp. (strain ATL72) has been reported to be a promising producer of high concentrations of carotenoids. A combination of central composite design (CCD) and Plackett–Burman (PB) design was used to optimize carotenoids produced by this yeast. The optimum production of carotenoids was completed when the yeast was grown in a production medium composed of 3.7 g/L malt extract, 7.7 g/L fructose, 9 g/L urea, 35 g/L NaCl, and 1 g/L yeast extract at 27.5 °C, pH 6.7, and 180 rpm. Two batch runs in 1 L and 7 L bioreactors were conducted which increased the productivity of carotenoid concentration from 21.5 mg/L after 98 h of incubation at the level of the shake flask to 229.9 mg/L after 47 h of incubation at the level of 7 L bioreactor. The carotenoid pigment was extracted in dimethylsulfoxide (DMSO), acetone, petroleum ether, and sodium chloride, and subsequently identified and characterized using UV-visible scanning, thin layer chromatography, and gas chromatography/mass spectrometry. |
| first_indexed | 2024-03-09T21:38:27Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T21:38:27Z |
| publishDate | 2022-02-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-b181001c148e4cefa9af9e5a8eb07dd02023-11-23T20:37:47ZengMDPI AGJournal of Fungi2309-608X2022-02-018216010.3390/jof8020160Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological ApplicationsAmira Dyaa0Hoda Soliman1Ahmed Abdelrazak2Bassem N. Samra3Ebtihal Khojah4Atef F. Ahmed5Mohamed A. El-Esawi6Ashraf Elsayed7Botany Departement, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, EgyptBotany Departement, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, EgyptBotany Departement, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, EgyptDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Food Science and Nutrition, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBotany Department, Faculty of Science, Tanta University, Tanta 31527, EgyptBotany Departement, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, Egypt<i>Rhodotorula</i> yeasts which are known as carotenogenic yeasts have a great industrial value due to their ability to produce carotenoids. In particular, the isolated yeast <i>Rhodotorula</i> sp. (strain ATL72) has been reported to be a promising producer of high concentrations of carotenoids. A combination of central composite design (CCD) and Plackett–Burman (PB) design was used to optimize carotenoids produced by this yeast. The optimum production of carotenoids was completed when the yeast was grown in a production medium composed of 3.7 g/L malt extract, 7.7 g/L fructose, 9 g/L urea, 35 g/L NaCl, and 1 g/L yeast extract at 27.5 °C, pH 6.7, and 180 rpm. Two batch runs in 1 L and 7 L bioreactors were conducted which increased the productivity of carotenoid concentration from 21.5 mg/L after 98 h of incubation at the level of the shake flask to 229.9 mg/L after 47 h of incubation at the level of 7 L bioreactor. The carotenoid pigment was extracted in dimethylsulfoxide (DMSO), acetone, petroleum ether, and sodium chloride, and subsequently identified and characterized using UV-visible scanning, thin layer chromatography, and gas chromatography/mass spectrometry.https://www.mdpi.com/2309-608X/8/2/160carotenogenic yeastPlackett–Burmancentral composite design |
| spellingShingle | Amira Dyaa Hoda Soliman Ahmed Abdelrazak Bassem N. Samra Ebtihal Khojah Atef F. Ahmed Mohamed A. El-Esawi Ashraf Elsayed Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications Journal of Fungi carotenogenic yeast Plackett–Burman central composite design |
| title | Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications |
| title_full | Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications |
| title_fullStr | Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications |
| title_full_unstemmed | Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications |
| title_short | Optimization of Carotenoids Production from <i>Rhodotorula</i> sp. Strain ATL72 for Enhancing Its Biotechnological Applications |
| title_sort | optimization of carotenoids production from i rhodotorula i sp strain atl72 for enhancing its biotechnological applications |
| topic | carotenogenic yeast Plackett–Burman central composite design |
| url | https://www.mdpi.com/2309-608X/8/2/160 |
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