Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities
Polyphenols are functional compounds in plants, which possess many bioactivities beneficial for humans. The aim of this study was to establish a highly efficient method for extracting polyphenol compounds from areca seeds and further to identify polyphenols and antioxidant properties of the seeds. A...
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| Format: | Article |
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MDPI AG
2014-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/10/16416 |
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| author | Wei-Min Zhang Wu-Yang Huang Wen-Xue Chen Lin Han Hai-De Zhang |
| author_facet | Wei-Min Zhang Wu-Yang Huang Wen-Xue Chen Lin Han Hai-De Zhang |
| author_sort | Wei-Min Zhang |
| collection | DOAJ |
| description | Polyphenols are functional compounds in plants, which possess many bioactivities beneficial for humans. The aim of this study was to establish a highly efficient method for extracting polyphenol compounds from areca seeds and further to identify polyphenols and antioxidant properties of the seeds. A quadratic general rotary unitized design was used to determine the optimal extraction process. The polyphenols were identified using LC-TOF-MS. By comparison with ascorbic acid (Vc), the antioxidant activities of the ethanol extracts were evaluated using three complementary in vitro assays: inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, hydroxyl radical-scavenging activity, and reducing ability. The two major polyphenols obtained were epicatechin and syringic acid. The ethanol extracts of areca seeds showed significantly greater antioxidant activity (p < 0.05) than Vc using the DPPH and reducing power assay, but lower ability (p < 0.05) using the hydroxyl radical assay. The results indicate that the areca seed is an excellent food material with potential antioxidant properties. |
| first_indexed | 2024-12-10T19:48:30Z |
| format | Article |
| id | doaj.art-b1861b69220d4734b5dbb60e7dde2cb5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T19:48:30Z |
| publishDate | 2014-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b1861b69220d4734b5dbb60e7dde2cb52022-12-22T01:35:50ZengMDPI AGMolecules1420-30492014-10-011910164161642710.3390/molecules191016416molecules191016416Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant ActivitiesWei-Min Zhang0Wu-Yang Huang1Wen-Xue Chen2Lin Han3Hai-De Zhang4College of Food Science, Hainan University, Haikou 570228, ChinaJiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaCollege of Food Science, Hainan University, Haikou 570228, ChinaCollege of Food Science, Hainan University, Haikou 570228, ChinaCollege of Food Science, Hainan University, Haikou 570228, ChinaPolyphenols are functional compounds in plants, which possess many bioactivities beneficial for humans. The aim of this study was to establish a highly efficient method for extracting polyphenol compounds from areca seeds and further to identify polyphenols and antioxidant properties of the seeds. A quadratic general rotary unitized design was used to determine the optimal extraction process. The polyphenols were identified using LC-TOF-MS. By comparison with ascorbic acid (Vc), the antioxidant activities of the ethanol extracts were evaluated using three complementary in vitro assays: inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, hydroxyl radical-scavenging activity, and reducing ability. The two major polyphenols obtained were epicatechin and syringic acid. The ethanol extracts of areca seeds showed significantly greater antioxidant activity (p < 0.05) than Vc using the DPPH and reducing power assay, but lower ability (p < 0.05) using the hydroxyl radical assay. The results indicate that the areca seed is an excellent food material with potential antioxidant properties.http://www.mdpi.com/1420-3049/19/10/16416antioxidant activitiesAreca catechu L.extractionpolyphenols |
| spellingShingle | Wei-Min Zhang Wu-Yang Huang Wen-Xue Chen Lin Han Hai-De Zhang Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities Molecules antioxidant activities Areca catechu L. extraction polyphenols |
| title | Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities |
| title_full | Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities |
| title_fullStr | Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities |
| title_full_unstemmed | Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities |
| title_short | Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities |
| title_sort | optimization of extraction conditions of areca seed polyphenols and evaluation of their antioxidant activities |
| topic | antioxidant activities Areca catechu L. extraction polyphenols |
| url | http://www.mdpi.com/1420-3049/19/10/16416 |
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