Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey
To develop and subsequently get cancer researchers to use organotypic three-dimensional (3D) models that can recapitulate the complexity of human in vivo tumors in an in vitro setting, it is important to establish what in vitro model(s) researchers are currently using and the reasons why. Thus, we d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/13/23/6033 |
| _version_ | 1797508006575865856 |
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| author | Sarai Martinez-Pacheco Lorraine O’Driscoll |
| author_facet | Sarai Martinez-Pacheco Lorraine O’Driscoll |
| author_sort | Sarai Martinez-Pacheco |
| collection | DOAJ |
| description | To develop and subsequently get cancer researchers to use organotypic three-dimensional (3D) models that can recapitulate the complexity of human in vivo tumors in an in vitro setting, it is important to establish what in vitro model(s) researchers are currently using and the reasons why. Thus, we developed a survey on this topic, obtained ethics approval, and circulated it throughout the world. The survey was completed by 101 researchers, across all career stages, in academia, clinical or industry settings. It included 40 questions, many with multiple options. Respondents reported on their field of cancer research; type of cancers studied; use of two-dimensional (2D)/monolayer, 2.5D and/or 3D cultures; if using co-cultures, the cell types(s) they co-culture; if using 3D cultures, whether these involve culturing the cells in a particular way to generate spheroids, or if they use additional supports/scaffolds; techniques used to analyze the 2D/2.5D/3D; and their downstream applications. Most researchers (>66%) only use 2D cultures, mainly due to lack of experience and costs. Despite most cancer researchers currently not using the 3D format, >80% recognize their importance and would like to progress to using 3D models. This suggests an urgent need to standardize reliable, robust, reproducible methods for establishing cost-effective 3D cell culture models and their subsequent characterization. |
| first_indexed | 2024-03-10T04:56:29Z |
| format | Article |
| id | doaj.art-b18b1ff338ae41fdbef108c687b09fc5 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T04:56:29Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b18b1ff338ae41fdbef108c687b09fc52023-11-23T02:13:25ZengMDPI AGCancers2072-66942021-11-011323603310.3390/cancers13236033Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide SurveySarai Martinez-Pacheco0Lorraine O’Driscoll1School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, Trinity College Dublin, D02 PN40 Dublin, IrelandSchool of Pharmacy and Pharmaceutical Sciences, Panoz Institute, Trinity College Dublin, D02 PN40 Dublin, IrelandTo develop and subsequently get cancer researchers to use organotypic three-dimensional (3D) models that can recapitulate the complexity of human in vivo tumors in an in vitro setting, it is important to establish what in vitro model(s) researchers are currently using and the reasons why. Thus, we developed a survey on this topic, obtained ethics approval, and circulated it throughout the world. The survey was completed by 101 researchers, across all career stages, in academia, clinical or industry settings. It included 40 questions, many with multiple options. Respondents reported on their field of cancer research; type of cancers studied; use of two-dimensional (2D)/monolayer, 2.5D and/or 3D cultures; if using co-cultures, the cell types(s) they co-culture; if using 3D cultures, whether these involve culturing the cells in a particular way to generate spheroids, or if they use additional supports/scaffolds; techniques used to analyze the 2D/2.5D/3D; and their downstream applications. Most researchers (>66%) only use 2D cultures, mainly due to lack of experience and costs. Despite most cancer researchers currently not using the 3D format, >80% recognize their importance and would like to progress to using 3D models. This suggests an urgent need to standardize reliable, robust, reproducible methods for establishing cost-effective 3D cell culture models and their subsequent characterization.https://www.mdpi.com/2072-6694/13/23/6033cancerin vitro models3D culture3Rsmethodologysurvey |
| spellingShingle | Sarai Martinez-Pacheco Lorraine O’Driscoll Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey Cancers cancer in vitro models 3D culture 3Rs methodology survey |
| title | Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey |
| title_full | Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey |
| title_fullStr | Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey |
| title_full_unstemmed | Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey |
| title_short | Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey |
| title_sort | pre clinical in vitro models used in cancer research results of a worldwide survey |
| topic | cancer in vitro models 3D culture 3Rs methodology survey |
| url | https://www.mdpi.com/2072-6694/13/23/6033 |
| work_keys_str_mv | AT saraimartinezpacheco preclinicalinvitromodelsusedincancerresearchresultsofaworldwidesurvey AT lorraineodriscoll preclinicalinvitromodelsusedincancerresearchresultsofaworldwidesurvey |