Simple protocol for population (Sanger) sequencing for Zika virus genomic regions

Simple protocol for population (Sanger) sequencing for Zika virus genomic regions

BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECT...

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Bibliographic Details
Main Authors: Gabriela Bastos Cabral, João Leandro de Paula Ferreira, Renato Pereira de Souza, Mariana Sequetin Cunha, Adriana Luchs, Cristina Adelaide Figueiredo, Luís Fernando de Macedo Brígido
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2017-11-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
Zika virus
Sanger sequencing
PCR
phylogeny
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000100038&lng=en&tlng=en

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