Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation
PurposeImmune escaping from host herd immunity has been related to changes in viral genomic sequences. The study aimed to understand the diverse immune responses to different subtypes or genotypes of human respiratory syncytial virus (RSV) in pediatric patients.MethodsThe genomic sequences of differ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1084139/full |
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| author | Xiaohe Zhou Xiaohe Zhou Mingli Jiang Mingli Jiang Fengjie Wang Fengjie Wang Yuan Qian Yuan Qian Qinwei Song Yu Sun Yu Sun Runan Zhu Runan Zhu Fang Wang Fang Wang Dong Qu Ling Cao Lijuan Ma Yanpeng Xu Yanpeng Xu Ri De Ri De Linqing Zhao Linqing Zhao |
| author_facet | Xiaohe Zhou Xiaohe Zhou Mingli Jiang Mingli Jiang Fengjie Wang Fengjie Wang Yuan Qian Yuan Qian Qinwei Song Yu Sun Yu Sun Runan Zhu Runan Zhu Fang Wang Fang Wang Dong Qu Ling Cao Lijuan Ma Yanpeng Xu Yanpeng Xu Ri De Ri De Linqing Zhao Linqing Zhao |
| author_sort | Xiaohe Zhou |
| collection | DOAJ |
| description | PurposeImmune escaping from host herd immunity has been related to changes in viral genomic sequences. The study aimed to understand the diverse immune responses to different subtypes or genotypes of human respiratory syncytial virus (RSV) in pediatric patients.MethodsThe genomic sequences of different subtypes or RSV genotypes, isolated from Beijing patients, were sequenced and systematically analyzed. Specifically, the antiviral effects of Palivizumab and the cross-reactivity of human sera from RSV-positive patients to different subtypes or genotypes of RSV were determined. Then, the level of 38 cytokines and chemokines in respiratory and serum samples from RSV-positive patients was evaluated.ResultsThe highest nucleotide and amino acid variations and the secondary and tertiary structure diversities among different subtypes or genotypes of RSV were found in G, especially for genotype ON1 with a 72bp-insertion compared to NA1 in subtype A, while more mutations of F protein were found in the NH-2 terminal, including the antigenic site II, the target of Palivizumab, containing one change N276S. Palivizumab inhibited subtype A with higher efficiency than subtype B and had stronger inhibitory effects on the reference strains than on isolated strains. However, RSV-positive sera had stronger inhibitory effects on the strains in the same subtypes or genotypes of RSV. The level of IFN-α2, IL-1α, and IL-1β in respiratory specimens from patients with NA1 was lower than those with ON1, while there were higher TNFα, IFNγ, IL-1α, and IL-1β in the first serum samples from patients with ON1 compared to those with BA9 of subtype B.ConclusionsDiverse host immune responses were correlated with differential subtypes and genotypes of RSV in pediatric patients, demonstrating the impact of viral genetics on host immunity. |
| first_indexed | 2024-04-10T23:50:32Z |
| format | Article |
| id | doaj.art-b191b748eb6b4299a701b1d01d368936 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T23:50:32Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-b191b748eb6b4299a701b1d01d3689362023-01-10T19:50:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-01-011310.3389/fimmu.2022.10841391084139Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variationXiaohe Zhou0Xiaohe Zhou1Mingli Jiang2Mingli Jiang3Fengjie Wang4Fengjie Wang5Yuan Qian6Yuan Qian7Qinwei Song8Yu Sun9Yu Sun10Runan Zhu11Runan Zhu12Fang Wang13Fang Wang14Dong Qu15Ling Cao16Lijuan Ma17Yanpeng Xu18Yanpeng Xu19Ri De20Ri De21Linqing Zhao22Linqing Zhao23Laboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaClinical Laboratory, Affiliated Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaIntensive Care Unit, Affiliated Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Respiratory, Affiliated Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaClinical Laboratory, Affiliated Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaLaboratory of Virology, Capital Institute of Pediatrics, Beijing, ChinaBeijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, ChinaPurposeImmune escaping from host herd immunity has been related to changes in viral genomic sequences. The study aimed to understand the diverse immune responses to different subtypes or genotypes of human respiratory syncytial virus (RSV) in pediatric patients.MethodsThe genomic sequences of different subtypes or RSV genotypes, isolated from Beijing patients, were sequenced and systematically analyzed. Specifically, the antiviral effects of Palivizumab and the cross-reactivity of human sera from RSV-positive patients to different subtypes or genotypes of RSV were determined. Then, the level of 38 cytokines and chemokines in respiratory and serum samples from RSV-positive patients was evaluated.ResultsThe highest nucleotide and amino acid variations and the secondary and tertiary structure diversities among different subtypes or genotypes of RSV were found in G, especially for genotype ON1 with a 72bp-insertion compared to NA1 in subtype A, while more mutations of F protein were found in the NH-2 terminal, including the antigenic site II, the target of Palivizumab, containing one change N276S. Palivizumab inhibited subtype A with higher efficiency than subtype B and had stronger inhibitory effects on the reference strains than on isolated strains. However, RSV-positive sera had stronger inhibitory effects on the strains in the same subtypes or genotypes of RSV. The level of IFN-α2, IL-1α, and IL-1β in respiratory specimens from patients with NA1 was lower than those with ON1, while there were higher TNFα, IFNγ, IL-1α, and IL-1β in the first serum samples from patients with ON1 compared to those with BA9 of subtype B.ConclusionsDiverse host immune responses were correlated with differential subtypes and genotypes of RSV in pediatric patients, demonstrating the impact of viral genetics on host immunity.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1084139/fullhuman respiratory syncytial virusviral genetic differencesantiviral effectschemokines and cytokinesimmune evasion |
| spellingShingle | Xiaohe Zhou Xiaohe Zhou Mingli Jiang Mingli Jiang Fengjie Wang Fengjie Wang Yuan Qian Yuan Qian Qinwei Song Yu Sun Yu Sun Runan Zhu Runan Zhu Fang Wang Fang Wang Dong Qu Ling Cao Lijuan Ma Yanpeng Xu Yanpeng Xu Ri De Ri De Linqing Zhao Linqing Zhao Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation Frontiers in Immunology human respiratory syncytial virus viral genetic differences antiviral effects chemokines and cytokines immune evasion |
| title | Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation |
| title_full | Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation |
| title_fullStr | Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation |
| title_full_unstemmed | Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation |
| title_short | Immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation |
| title_sort | immune escaping of the novel genotypes of human respiratory syncytial virus based on gene sequence variation |
| topic | human respiratory syncytial virus viral genetic differences antiviral effects chemokines and cytokines immune evasion |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1084139/full |
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