Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets
A total of 306 isolates of lactic acid bacteria were isolated from 250 samples of piglet faeces. The strains were investigated for preliminary probiotic properties based on their stability in bile salts (0.30%)and high acidity (pH 3.0). Ability to utilize protein, fat and starch, growth in the absen...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2007-05-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/29_Suppl2_grad/0125-3395-29-S2-0269-0279.pdf |
| _version_ | 1818522245461442560 |
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| author | Malawach, T. Kantachote, D. Charernjiratrakul, W. |
| author_facet | Malawach, T. Kantachote, D. Charernjiratrakul, W. |
| author_sort | Malawach, T. |
| collection | DOAJ |
| description | A total of 306 isolates of lactic acid bacteria were isolated from 250 samples of piglet faeces. The strains were investigated for preliminary probiotic properties based on their stability in bile salts (0.30%)and high acidity (pH 3.0). Ability to utilize protein, fat and starch, growth in the absence of vitamin B12 and growth with both aerobic and anaerobic conditions were also considered. As a result of above criteria, 20isolates were selected. Using an agar spot method, all isolates were able to inhibit β-hemolytic Escherichia coli 240/2 under aerobic and anaerobic condition. A further investigation using a co-culture techniqueshowed that only six isolates inhibited β-hemolytic E. coli 240/2, E. coli K88 and E. coli K 99 by more than 90 percent. The selected isolates were resistant to the following antibiotics: amikacin, polymyxin B andnalidixic acid; however the strains were susceptible to erythromycin and chloramphenicol. All six active isolates were identified as Lactobacillus plantarum by API 50 CH system. |
| first_indexed | 2024-12-11T05:30:43Z |
| format | Article |
| id | doaj.art-b19468f5d8834a9982a1b08297cce50b |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-11T05:30:43Z |
| publishDate | 2007-05-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-b19468f5d8834a9982a1b08297cce50b2022-12-22T01:19:26ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952007-05-0129Suppl.2269279Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal pigletsMalawach, T.Kantachote, D.Charernjiratrakul, W.A total of 306 isolates of lactic acid bacteria were isolated from 250 samples of piglet faeces. The strains were investigated for preliminary probiotic properties based on their stability in bile salts (0.30%)and high acidity (pH 3.0). Ability to utilize protein, fat and starch, growth in the absence of vitamin B12 and growth with both aerobic and anaerobic conditions were also considered. As a result of above criteria, 20isolates were selected. Using an agar spot method, all isolates were able to inhibit β-hemolytic Escherichia coli 240/2 under aerobic and anaerobic condition. A further investigation using a co-culture techniqueshowed that only six isolates inhibited β-hemolytic E. coli 240/2, E. coli K88 and E. coli K 99 by more than 90 percent. The selected isolates were resistant to the following antibiotics: amikacin, polymyxin B andnalidixic acid; however the strains were susceptible to erythromycin and chloramphenicol. All six active isolates were identified as Lactobacillus plantarum by API 50 CH system.http://www.sjst.psu.ac.th/journal/29_Suppl2_grad/0125-3395-29-S2-0269-0279.pdflactic acid bacteriaprobioticEscherichia coli |
| spellingShingle | Malawach, T. Kantachote, D. Charernjiratrakul, W. Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets Songklanakarin Journal of Science and Technology (SJST) lactic acid bacteria probiotic Escherichia coli |
| title | Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets |
| title_full | Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets |
| title_fullStr | Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets |
| title_full_unstemmed | Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets |
| title_short | Selection of probiotic lactic acid bacteria able to inhibit β-hemolytic Escherichia coli isolated from diarrheal piglets |
| title_sort | selection of probiotic lactic acid bacteria able to inhibit β hemolytic escherichia coli isolated from diarrheal piglets |
| topic | lactic acid bacteria probiotic Escherichia coli |
| url | http://www.sjst.psu.ac.th/journal/29_Suppl2_grad/0125-3395-29-S2-0269-0279.pdf |
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