Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum
Data on the quantification of the potentially neurotrophic adipo-myokine METRNL (Meteorin-like protein) in human cerebrospinal fluid (CSF) are lacking and migration of this secreted protein across the blood–brain barrier (BBB) is uncertain. In the present pilot study, METRNL concentrations were quan...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/15/3271 |
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| author | Martin Berghoff Alexandra Höpfinger Ranjithkumar Rajendran Thomas Karrasch Andreas Schmid Andreas Schäffler |
| author_facet | Martin Berghoff Alexandra Höpfinger Ranjithkumar Rajendran Thomas Karrasch Andreas Schmid Andreas Schäffler |
| author_sort | Martin Berghoff |
| collection | DOAJ |
| description | Data on the quantification of the potentially neurotrophic adipo-myokine METRNL (Meteorin-like protein) in human cerebrospinal fluid (CSF) are lacking and migration of this secreted protein across the blood–brain barrier (BBB) is uncertain. In the present pilot study, METRNL concentrations were quantified by ELISA in paired serum and CSF samples of 260 patients (107 males, 153 females) undergoing neurological evaluation. METRNL was abundant in serum (801.2 ± 378.3 pg/mL) and CSF (1007.2 ± 624.2 pg/mL) with a CSF/serum ratio of 1.4 ± 0.8. Serum METRNL levels were significantly correlated (rho = +0.521) to those in CSF. CSF METRNL concentrations were significantly correlated (rho = +0.480) with albumin CSF/serum ratios. The CSF/serum ratios of METRNL and albumin were positively correlated in Reibergram analysis (rho = 0.498), indicating that raising CSF concentrations of METRNL are mediated by increasing BBB dysfunction. The CSF concentrations of METRNL strongly increased in a stepwise manner along with increasing BBB dysfunction from grade 0 to grade 3 and with rising CSF cell count. CSF/serum ratio of METRNL also increased from grade 0 (1.2 ± 0.7) to grade 3 (3.0 ± 0.2). Furthermore, CSF levels were positively correlated with age. In conclusion, METRNL is a secreted and neurotrophic myokine that crosses over the BBB. CSF concentrations of METRNL increase with BBB dysfunction. |
| first_indexed | 2024-03-10T09:14:19Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T09:14:19Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-b194b0496a8a4ffba5c2e5a11ea002de2023-11-22T05:48:21ZengMDPI AGJournal of Clinical Medicine2077-03832021-07-011015327110.3390/jcm10153271Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and SerumMartin Berghoff0Alexandra Höpfinger1Ranjithkumar Rajendran2Thomas Karrasch3Andreas Schmid4Andreas Schäffler5Department of Neurology, Giessen University Hospital, 35392 Giessen, GermanyDepartment of Internal Medicine III, Giessen University Hospital, 35392 Giessen, GermanyDepartment of Neurology, Giessen University Hospital, 35392 Giessen, GermanyDepartment of Internal Medicine III, Giessen University Hospital, 35392 Giessen, GermanyDepartment of Internal Medicine III, Giessen University Hospital, 35392 Giessen, GermanyDepartment of Internal Medicine III, Giessen University Hospital, 35392 Giessen, GermanyData on the quantification of the potentially neurotrophic adipo-myokine METRNL (Meteorin-like protein) in human cerebrospinal fluid (CSF) are lacking and migration of this secreted protein across the blood–brain barrier (BBB) is uncertain. In the present pilot study, METRNL concentrations were quantified by ELISA in paired serum and CSF samples of 260 patients (107 males, 153 females) undergoing neurological evaluation. METRNL was abundant in serum (801.2 ± 378.3 pg/mL) and CSF (1007.2 ± 624.2 pg/mL) with a CSF/serum ratio of 1.4 ± 0.8. Serum METRNL levels were significantly correlated (rho = +0.521) to those in CSF. CSF METRNL concentrations were significantly correlated (rho = +0.480) with albumin CSF/serum ratios. The CSF/serum ratios of METRNL and albumin were positively correlated in Reibergram analysis (rho = 0.498), indicating that raising CSF concentrations of METRNL are mediated by increasing BBB dysfunction. The CSF concentrations of METRNL strongly increased in a stepwise manner along with increasing BBB dysfunction from grade 0 to grade 3 and with rising CSF cell count. CSF/serum ratio of METRNL also increased from grade 0 (1.2 ± 0.7) to grade 3 (3.0 ± 0.2). Furthermore, CSF levels were positively correlated with age. In conclusion, METRNL is a secreted and neurotrophic myokine that crosses over the BBB. CSF concentrations of METRNL increase with BBB dysfunction.https://www.mdpi.com/2077-0383/10/15/3271myokineMETRNL (Meteorin-like protein)cerebrospinal fluidblood–brain barrieradipokine |
| spellingShingle | Martin Berghoff Alexandra Höpfinger Ranjithkumar Rajendran Thomas Karrasch Andreas Schmid Andreas Schäffler Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum Journal of Clinical Medicine myokine METRNL (Meteorin-like protein) cerebrospinal fluid blood–brain barrier adipokine |
| title | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
| title_full | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
| title_fullStr | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
| title_full_unstemmed | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
| title_short | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
| title_sort | evidence of a muscle brain axis by quantification of the neurotrophic myokine metrnl meteorin like protein in human cerebrospinal fluid and serum |
| topic | myokine METRNL (Meteorin-like protein) cerebrospinal fluid blood–brain barrier adipokine |
| url | https://www.mdpi.com/2077-0383/10/15/3271 |
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