Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation
Background Ion homeostasis is an essential process for the survival of plants under salt stress. Na+/H+ antiporters (NHXs) are secondary ion transporters that regulate Na+ compartmentalization or efflux reduce Na+ toxicity and play a critical role during plant development and stress responses. Metho...
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PeerJ Inc.
2022-04-01
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| author | Luyao Huang Zhuangzhuang Li Chunyong Sun Shijie Yin Bin Wang Tongyao Duan Yang Liu Jia Li Gaobin Pu |
| author_facet | Luyao Huang Zhuangzhuang Li Chunyong Sun Shijie Yin Bin Wang Tongyao Duan Yang Liu Jia Li Gaobin Pu |
| author_sort | Luyao Huang |
| collection | DOAJ |
| description | Background Ion homeostasis is an essential process for the survival of plants under salt stress. Na+/H+ antiporters (NHXs) are secondary ion transporters that regulate Na+ compartmentalization or efflux reduce Na+ toxicity and play a critical role during plant development and stress responses. Methods and Results To gain insight into the functional divergence of NHX genes in honeysuckle, a total of seven LjNHX genes were identified on the whole genome level and were renamed according to their chromosomal positions. All LjNHXs possessed the Na+/H+ exchanger domain and the amiloride-binding site was presented in all NHX proteins except LjNHX4. The phylogenetic analysis divided the seven NHX genes into Vac-clade (LjNHX1/2/3/4/5/7) and PM-clade (LjNHX6) based on their subcellular localization and validated by the distribution of conserved protein motifs and exon/intron organization analysis. The protein-protein interaction network showed that LjNHX4/5/6/7 shared the same putatively interactive proteins, including SOS2, SOS3, HKT1, and AVP1. Cis-acting elements and gene ontology (GO) analysis suggested that most LjNHXs involve in the response to salt stress through ion transmembrane transport. The expression profile analysis revealed that the expression levels of LjNHX3/7 were remarkably affected by salinity. These results suggested that LjNHXs play significant roles in honeysuckle development and response to salt stresses. Conclusions The theoretical foundation was established in the present study for the further functional characterization of the NHX gene family in honeysuckle. |
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| spelling | doaj.art-b196056047894a619084b9427de668752023-12-03T10:02:53ZengPeerJ Inc.PeerJ2167-83592022-04-0110e1321410.7717/peerj.13214Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptationLuyao Huang0Zhuangzhuang Li1Chunyong Sun2Shijie Yin3Bin Wang4Tongyao Duan5Yang Liu6Jia Li7Gaobin Pu8Shandong University of Traditional Chinese Medicine, Jinan, ChinaOcean University of China, Qingdao, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaBackground Ion homeostasis is an essential process for the survival of plants under salt stress. Na+/H+ antiporters (NHXs) are secondary ion transporters that regulate Na+ compartmentalization or efflux reduce Na+ toxicity and play a critical role during plant development and stress responses. Methods and Results To gain insight into the functional divergence of NHX genes in honeysuckle, a total of seven LjNHX genes were identified on the whole genome level and were renamed according to their chromosomal positions. All LjNHXs possessed the Na+/H+ exchanger domain and the amiloride-binding site was presented in all NHX proteins except LjNHX4. The phylogenetic analysis divided the seven NHX genes into Vac-clade (LjNHX1/2/3/4/5/7) and PM-clade (LjNHX6) based on their subcellular localization and validated by the distribution of conserved protein motifs and exon/intron organization analysis. The protein-protein interaction network showed that LjNHX4/5/6/7 shared the same putatively interactive proteins, including SOS2, SOS3, HKT1, and AVP1. Cis-acting elements and gene ontology (GO) analysis suggested that most LjNHXs involve in the response to salt stress through ion transmembrane transport. The expression profile analysis revealed that the expression levels of LjNHX3/7 were remarkably affected by salinity. These results suggested that LjNHXs play significant roles in honeysuckle development and response to salt stresses. Conclusions The theoretical foundation was established in the present study for the further functional characterization of the NHX gene family in honeysuckle.https://peerj.com/articles/13214.pdfHoneysuckleGenome-wideNa+/H+ antiporter (NHX)Salt stress |
| spellingShingle | Luyao Huang Zhuangzhuang Li Chunyong Sun Shijie Yin Bin Wang Tongyao Duan Yang Liu Jia Li Gaobin Pu Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation PeerJ Honeysuckle Genome-wide Na+/H+ antiporter (NHX) Salt stress |
| title | Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation |
| title_full | Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation |
| title_fullStr | Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation |
| title_full_unstemmed | Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation |
| title_short | Genome-wide identification, molecular characterization, and gene expression analyses of honeysuckle NHX antiporters suggest their involvement in salt stress adaptation |
| title_sort | genome wide identification molecular characterization and gene expression analyses of honeysuckle nhx antiporters suggest their involvement in salt stress adaptation |
| topic | Honeysuckle Genome-wide Na+/H+ antiporter (NHX) Salt stress |
| url | https://peerj.com/articles/13214.pdf |
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