Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility
Two <i>Helichrysum italicum</i> extracts, OPT-1 (rich in phenolic acids) and OPT-2 (rich in total phenols and flavonoids), were prepared using hydroxypropyl-β-cyclodextrin (HP-β-CD)-assisted extraction. The prepared extracts were rich in phenolic compounds, including flavonoids and pheno...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/4/855 |
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| author | Lejsa Jakupović Ivana Bačić Jasna Jablan Eva Marguí Marijan Marijan Suzana Inić Laura Nižić Nodilo Anita Hafner Marijana Zovko Končić |
| author_facet | Lejsa Jakupović Ivana Bačić Jasna Jablan Eva Marguí Marijan Marijan Suzana Inić Laura Nižić Nodilo Anita Hafner Marijana Zovko Končić |
| author_sort | Lejsa Jakupović |
| collection | DOAJ |
| description | Two <i>Helichrysum italicum</i> extracts, OPT-1 (rich in phenolic acids) and OPT-2 (rich in total phenols and flavonoids), were prepared using hydroxypropyl-β-cyclodextrin (HP-β-CD)-assisted extraction. The prepared extracts were rich in phenolic compounds, including flavonoids and phenolic acids. GC-MS analysis of the extracts identified neryl acetate, neo-intermedeol, β-selinene, γ-curcumene, italidione I, and nerol as the main volatile components of the extracts, as well as plant sterols, γ-sitosterol, campesterol, and stigmasterol. The antioxidant (DPPH radical scavenging, reducing power, and a carotene linoleic acid assay) and cosmeceutical (anti-hyaluronidase, anti-tyrosinase, anti-lipoxygenase, ovalbumin anti-coagulation, and a UV-absorption assay) activity of the extracts in most of the assays was better than the activity of the applied positive controls. Especially low were the IC<sub>50</sub> values of the extracts in the anti-hyaluronidase (14.31 ± 0.29 μL extract/mL and 19.82 ± 1.53 μL extract/mL for OPT-1 and OPT-2, respectively) and the anti-lipoxygenase (0.96 ± 0.11 μL extract/mL and 1.07 ± 0.01 μL extract/mL for OPT-1 and OPT-2, respectively) assays. The extracts were non-toxic to HaCaT cells in concentrations of up to 62.5 µL extract/mL assuring their status as excellent candidates for cosmeceutical product development appropriate for direct use in cosmetic products without solvent evaporation. |
| first_indexed | 2024-03-11T05:18:16Z |
| format | Article |
| id | doaj.art-b196df75da824375893ac7fe72d68237 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T05:18:16Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b196df75da824375893ac7fe72d682372023-11-17T18:05:17ZengMDPI AGAntioxidants2076-39212023-04-0112485510.3390/antiox12040855Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and BiocompatibilityLejsa Jakupović0Ivana Bačić1Jasna Jablan2Eva Marguí3Marijan Marijan4Suzana Inić5Laura Nižić Nodilo6Anita Hafner7Marijana Zovko Končić8Department of Pharmacognosy, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaForensic Science Centre “Ivan Vučetić”, Ilica 335, 10000 Zagreb, CroatiaDepartment of Analytical Chemistry, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaDepartment of Chemistry, Faculty of Sciences, University of Girona, C/M. Aurèlia Capmany 69, 17003 Girona, SpainDepartment of Pharmacognosy, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaDepartment of Analytical Chemistry, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaDepartment of Pharmaceutical Technology, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaDepartment of Pharmaceutical Technology, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaDepartment of Pharmacognosy, University of Zagreb Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10000 Zagreb, CroatiaTwo <i>Helichrysum italicum</i> extracts, OPT-1 (rich in phenolic acids) and OPT-2 (rich in total phenols and flavonoids), were prepared using hydroxypropyl-β-cyclodextrin (HP-β-CD)-assisted extraction. The prepared extracts were rich in phenolic compounds, including flavonoids and phenolic acids. GC-MS analysis of the extracts identified neryl acetate, neo-intermedeol, β-selinene, γ-curcumene, italidione I, and nerol as the main volatile components of the extracts, as well as plant sterols, γ-sitosterol, campesterol, and stigmasterol. The antioxidant (DPPH radical scavenging, reducing power, and a carotene linoleic acid assay) and cosmeceutical (anti-hyaluronidase, anti-tyrosinase, anti-lipoxygenase, ovalbumin anti-coagulation, and a UV-absorption assay) activity of the extracts in most of the assays was better than the activity of the applied positive controls. Especially low were the IC<sub>50</sub> values of the extracts in the anti-hyaluronidase (14.31 ± 0.29 μL extract/mL and 19.82 ± 1.53 μL extract/mL for OPT-1 and OPT-2, respectively) and the anti-lipoxygenase (0.96 ± 0.11 μL extract/mL and 1.07 ± 0.01 μL extract/mL for OPT-1 and OPT-2, respectively) assays. The extracts were non-toxic to HaCaT cells in concentrations of up to 62.5 µL extract/mL assuring their status as excellent candidates for cosmeceutical product development appropriate for direct use in cosmetic products without solvent evaporation.https://www.mdpi.com/2076-3921/12/4/855anti-inflammatoryantioxidantcosmeceuticalHaCaT cells<i>Helichrysum italicum</i>hyaluronidase |
| spellingShingle | Lejsa Jakupović Ivana Bačić Jasna Jablan Eva Marguí Marijan Marijan Suzana Inić Laura Nižić Nodilo Anita Hafner Marijana Zovko Končić Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility Antioxidants anti-inflammatory antioxidant cosmeceutical HaCaT cells <i>Helichrysum italicum</i> hyaluronidase |
| title | Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility |
| title_full | Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility |
| title_fullStr | Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility |
| title_full_unstemmed | Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility |
| title_short | Hydroxypropyl-β-Cyclodextrin-Based <i>Helichrysum italicum</i> Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility |
| title_sort | hydroxypropyl β cyclodextrin based i helichrysum italicum i extracts antioxidant and cosmeceutical activity and biocompatibility |
| topic | anti-inflammatory antioxidant cosmeceutical HaCaT cells <i>Helichrysum italicum</i> hyaluronidase |
| url | https://www.mdpi.com/2076-3921/12/4/855 |
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