iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling.
Calcium independent group VIA phospholipase A(2) (iPLA(2)β) is up-regulated in vascular smooth muscle cells in some diseases, but whether the up-regulated iPLA(2)β affects vascular morphology and blood pressure is unknown. The current study addresses this question by evaluating the basal- and angiot...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3282780?pdf=render |
| _version_ | 1817977393962287104 |
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| author | Lindsay E Calderon Shu Liu Wen Su Zhongwen Xie Zhenheng Guo Wanda Eberhard Ming C Gong |
| author_facet | Lindsay E Calderon Shu Liu Wen Su Zhongwen Xie Zhenheng Guo Wanda Eberhard Ming C Gong |
| author_sort | Lindsay E Calderon |
| collection | DOAJ |
| description | Calcium independent group VIA phospholipase A(2) (iPLA(2)β) is up-regulated in vascular smooth muscle cells in some diseases, but whether the up-regulated iPLA(2)β affects vascular morphology and blood pressure is unknown. The current study addresses this question by evaluating the basal- and angiotensin II infusion-induced vascular remodeling and hypertension in smooth muscle specific iPLA(2)β transgenic (iPLA(2)β-Tg) mice.Blood pressure was monitored by radiotelemetry and vascular remodeling was assessed by morphologic analysis. We found that the angiotensin II-induced increase in diastolic pressure was significantly higher in iPLA(2)β-Tg than iPLA(2)β-Wt mice, whereas, the basal blood pressure was not significantly different. The media thickness and media∶lumen ratio of the mesenteric arteries were significantly increased in angiotensin II-infused iPLA(2)β-Tg mice. Analysis revealed no difference in vascular smooth muscle cell proliferation. In contrast, adenovirus-mediated iPLA(2)β overexpression in cultured vascular smooth muscle cells promoted angiotensin II-induced [(3)H]-leucine incorporation, indicating enhanced hypertrophy. Moreover, angiotensin II infusion-induced c-Jun phosphorylation in vascular smooth muscle cells overexpressing iPLA2β to higher levels, which was abolished by inhibition of 12/15 lipoxygenase. In addition, we found that angiotensin II up-regulated the endogenous iPLA(2)β protein in-vitro and in-vivo.The present study reports that iPLA(2)β up-regulation exacerbates angiotensin II-induced vascular smooth muscle cell hypertrophy, vascular remodeling and hypertension via the 12/15 lipoxygenase and c-Jun pathways. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T22:15:49Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-b199196c0a4741a0b7e650ef2de2af2d2022-12-22T02:27:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3185010.1371/journal.pone.0031850iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling.Lindsay E CalderonShu LiuWen SuZhongwen XieZhenheng GuoWanda EberhardMing C GongCalcium independent group VIA phospholipase A(2) (iPLA(2)β) is up-regulated in vascular smooth muscle cells in some diseases, but whether the up-regulated iPLA(2)β affects vascular morphology and blood pressure is unknown. The current study addresses this question by evaluating the basal- and angiotensin II infusion-induced vascular remodeling and hypertension in smooth muscle specific iPLA(2)β transgenic (iPLA(2)β-Tg) mice.Blood pressure was monitored by radiotelemetry and vascular remodeling was assessed by morphologic analysis. We found that the angiotensin II-induced increase in diastolic pressure was significantly higher in iPLA(2)β-Tg than iPLA(2)β-Wt mice, whereas, the basal blood pressure was not significantly different. The media thickness and media∶lumen ratio of the mesenteric arteries were significantly increased in angiotensin II-infused iPLA(2)β-Tg mice. Analysis revealed no difference in vascular smooth muscle cell proliferation. In contrast, adenovirus-mediated iPLA(2)β overexpression in cultured vascular smooth muscle cells promoted angiotensin II-induced [(3)H]-leucine incorporation, indicating enhanced hypertrophy. Moreover, angiotensin II infusion-induced c-Jun phosphorylation in vascular smooth muscle cells overexpressing iPLA2β to higher levels, which was abolished by inhibition of 12/15 lipoxygenase. In addition, we found that angiotensin II up-regulated the endogenous iPLA(2)β protein in-vitro and in-vivo.The present study reports that iPLA(2)β up-regulation exacerbates angiotensin II-induced vascular smooth muscle cell hypertrophy, vascular remodeling and hypertension via the 12/15 lipoxygenase and c-Jun pathways.http://europepmc.org/articles/PMC3282780?pdf=render |
| spellingShingle | Lindsay E Calderon Shu Liu Wen Su Zhongwen Xie Zhenheng Guo Wanda Eberhard Ming C Gong iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling. PLoS ONE |
| title | iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling. |
| title_full | iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling. |
| title_fullStr | iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling. |
| title_full_unstemmed | iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling. |
| title_short | iPLA2β overexpression in smooth muscle exacerbates angiotensin II-induced hypertension and vascular remodeling. |
| title_sort | ipla2β overexpression in smooth muscle exacerbates angiotensin ii induced hypertension and vascular remodeling |
| url | http://europepmc.org/articles/PMC3282780?pdf=render |
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