Disinfection performance and synthesis conditions of the EGCG–Cu complex
Adopting the yield of the epicatechin gallate (EGCG)–Cu complex as an indicator, the impact of four conditions, such as pH, reaction time, temperature and reactant ratio, on the synthesis of the target complex was analyzed to identify the optimal conditions on synthesizing the compound. The reactant...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IWA Publishing
2023-12-01
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| Series: | Water Supply |
| Subjects: | |
| Online Access: | http://ws.iwaponline.com/content/23/12/5077 |
| _version_ | 1797368609266204672 |
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| author | Cuimin Feng Sairui Guan JiYue Jin Renda Yao Ziwei Wang Haoxiang Li |
| author_facet | Cuimin Feng Sairui Guan JiYue Jin Renda Yao Ziwei Wang Haoxiang Li |
| author_sort | Cuimin Feng |
| collection | DOAJ |
| description | Adopting the yield of the epicatechin gallate (EGCG)–Cu complex as an indicator, the impact of four conditions, such as pH, reaction time, temperature and reactant ratio, on the synthesis of the target complex was analyzed to identify the optimal conditions on synthesizing the compound. The reactant ratio between EGCG and Cu2+ was explored, and the characteristic peaks and functional groups of the EGCG–Cu complex were scrutinized by UV–Visible and Fourier-Transform Infrared spectrophotometers. Finally, the efficacy difference of bactericidal properties against Escherichia coli suspensions between EGCG and its complex was evaluated as the criteria. Furthermore, comparative studies were performed to gauge the antimicrobial activity of EGCG and its complex at equivalent concentrations. The results demonstrated that the optimal experimental conditions for the complex reaction were a pH of 7, at 40 °C, in a reaction time of 60 min and a reactant ratio of 1:2. By the molar ratio method, the reactant ratio was determined as n(EGCG):n(Cu2+) = 1:2, and the complex reaction was at the phenolic hydroxyl group on the benzene ring of EGCG. Compared to EGCG, the complex demonstrated significant enhancement in bactericidal properties against E. coli and has lower chromaticity.
HIGHLIGHTS
The optimal parameters for complexation were determined.;
The absorbance curve showed that the complex ratio of EGCG and Cu2+ was 1:2.;
The changes in absorption peaks of functional groups indicated that EGCG's phenolic hydroxyl groups on the benzene ring formed complexes.;
Complexing Cu2+ to EGCG enhances its antibacterial properties.;
The EGCG–Cu complex solution has lower chromaticity than EGCG during disinfection.; |
| first_indexed | 2024-03-08T17:35:13Z |
| format | Article |
| id | doaj.art-b19a3f9e7f544a758f64d77bc1398ad4 |
| institution | Directory Open Access Journal |
| issn | 1606-9749 1607-0798 |
| language | English |
| last_indexed | 2024-03-08T17:35:13Z |
| publishDate | 2023-12-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Supply |
| spelling | doaj.art-b19a3f9e7f544a758f64d77bc1398ad42024-01-02T13:17:33ZengIWA PublishingWater Supply1606-97491607-07982023-12-0123125077509210.2166/ws.2023.302302Disinfection performance and synthesis conditions of the EGCG–Cu complexCuimin Feng0Sairui Guan1JiYue Jin2Renda Yao3Ziwei Wang4Haoxiang Li5 National Demonstration Center for Experimental Water Environment Education, Beijing University of Civil Engineering and Architecture, Beijing 100044, China National Demonstration Center for Experimental Water Environment Education, Beijing University of Civil Engineering and Architecture, Beijing 100044, China Beijing Waterworks Group Co., Ltd., Beijing 100031, China Research Institute for Environmental Innovation (Tianjin Binhai), Tianjin 300457, China National Demonstration Center for Experimental Water Environment Education, Beijing University of Civil Engineering and Architecture, Beijing 100044, China National Demonstration Center for Experimental Water Environment Education, Beijing University of Civil Engineering and Architecture, Beijing 100044, China Adopting the yield of the epicatechin gallate (EGCG)–Cu complex as an indicator, the impact of four conditions, such as pH, reaction time, temperature and reactant ratio, on the synthesis of the target complex was analyzed to identify the optimal conditions on synthesizing the compound. The reactant ratio between EGCG and Cu2+ was explored, and the characteristic peaks and functional groups of the EGCG–Cu complex were scrutinized by UV–Visible and Fourier-Transform Infrared spectrophotometers. Finally, the efficacy difference of bactericidal properties against Escherichia coli suspensions between EGCG and its complex was evaluated as the criteria. Furthermore, comparative studies were performed to gauge the antimicrobial activity of EGCG and its complex at equivalent concentrations. The results demonstrated that the optimal experimental conditions for the complex reaction were a pH of 7, at 40 °C, in a reaction time of 60 min and a reactant ratio of 1:2. By the molar ratio method, the reactant ratio was determined as n(EGCG):n(Cu2+) = 1:2, and the complex reaction was at the phenolic hydroxyl group on the benzene ring of EGCG. Compared to EGCG, the complex demonstrated significant enhancement in bactericidal properties against E. coli and has lower chromaticity. HIGHLIGHTS The optimal parameters for complexation were determined.; The absorbance curve showed that the complex ratio of EGCG and Cu2+ was 1:2.; The changes in absorption peaks of functional groups indicated that EGCG's phenolic hydroxyl groups on the benzene ring formed complexes.; Complexing Cu2+ to EGCG enhances its antibacterial properties.; The EGCG–Cu complex solution has lower chromaticity than EGCG during disinfection.;http://ws.iwaponline.com/content/23/12/5077complexcopper iondisinfectiondrinking wateregcgmolar ratio |
| spellingShingle | Cuimin Feng Sairui Guan JiYue Jin Renda Yao Ziwei Wang Haoxiang Li Disinfection performance and synthesis conditions of the EGCG–Cu complex Water Supply complex copper ion disinfection drinking water egcg molar ratio |
| title | Disinfection performance and synthesis conditions of the EGCG–Cu complex |
| title_full | Disinfection performance and synthesis conditions of the EGCG–Cu complex |
| title_fullStr | Disinfection performance and synthesis conditions of the EGCG–Cu complex |
| title_full_unstemmed | Disinfection performance and synthesis conditions of the EGCG–Cu complex |
| title_short | Disinfection performance and synthesis conditions of the EGCG–Cu complex |
| title_sort | disinfection performance and synthesis conditions of the egcg cu complex |
| topic | complex copper ion disinfection drinking water egcg molar ratio |
| url | http://ws.iwaponline.com/content/23/12/5077 |
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