The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments

ABSTRACT Objectives: to determine if there are differences on the safety of the preparation of clean surgical instruments using different types of gloves and bare hands and evaluate the microbiological load of these preparations without gloves. Method: laboratory procedure with a pragmatic appro...

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Main Authors: Camila Quartim de Moraes Bruna, Rafael Queiroz de Souza, Irineu Francisco Silva Massaia, Áurea Silveira Cruz, Kazuko Uchikawa Graziano
Format: Article
Language:English
Published: Universidade de São Paulo
Series:Revista Latino-Americana de Enfermagem
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-11692016000100417&lng=en&tlng=en
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author Camila Quartim de Moraes Bruna
Rafael Queiroz de Souza
Irineu Francisco Silva Massaia
Áurea Silveira Cruz
Kazuko Uchikawa Graziano
author_facet Camila Quartim de Moraes Bruna
Rafael Queiroz de Souza
Irineu Francisco Silva Massaia
Áurea Silveira Cruz
Kazuko Uchikawa Graziano
author_sort Camila Quartim de Moraes Bruna
collection DOAJ
description ABSTRACT Objectives: to determine if there are differences on the safety of the preparation of clean surgical instruments using different types of gloves and bare hands and evaluate the microbiological load of these preparations without gloves. Method: laboratory procedure with a pragmatic approach, in which the samples were handled with different types of gloves and bare hands. In addition, cytotoxicity assays were carried out by means of the agar diffusion method. Further samples were subjected to microbiological analysis after being handled without gloves. Results: none of the samples showed cytotoxic effect. All microbiological cultures showed growth of microorganisms, but no microorganism has been recovered after autoclaving. Conclusion: there were no differences in the cytotoxic responses regarding the use of different types of gloves and bare hands in the handling of clean surgical instruments, which could entail iatrogenic risk. It is noteworthy that the use of gloves involves increase in the costs of process and waste generation, and the potential allergenic risk to latex.
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spelling doaj.art-b19b24c1b6b24283845927d2695ebe572022-12-22T03:43:59ZengUniversidade de São PauloRevista Latino-Americana de Enfermagem1518-834524010.1590/1518-8345.1127.2805S0104-11692016000100417The impact of the use of different types of gloves and bare hands for preparation of clean surgical instrumentsCamila Quartim de Moraes BrunaRafael Queiroz de SouzaIrineu Francisco Silva MassaiaÁurea Silveira CruzKazuko Uchikawa GrazianoABSTRACT Objectives: to determine if there are differences on the safety of the preparation of clean surgical instruments using different types of gloves and bare hands and evaluate the microbiological load of these preparations without gloves. Method: laboratory procedure with a pragmatic approach, in which the samples were handled with different types of gloves and bare hands. In addition, cytotoxicity assays were carried out by means of the agar diffusion method. Further samples were subjected to microbiological analysis after being handled without gloves. Results: none of the samples showed cytotoxic effect. All microbiological cultures showed growth of microorganisms, but no microorganism has been recovered after autoclaving. Conclusion: there were no differences in the cytotoxic responses regarding the use of different types of gloves and bare hands in the handling of clean surgical instruments, which could entail iatrogenic risk. It is noteworthy that the use of gloves involves increase in the costs of process and waste generation, and the potential allergenic risk to latex.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-11692016000100417&lng=en&tlng=enResíduos OrgánicosInstrumentos QuirúrgicosManoGuantes ProtectoresToxicidad;
spellingShingle Camila Quartim de Moraes Bruna
Rafael Queiroz de Souza
Irineu Francisco Silva Massaia
Áurea Silveira Cruz
Kazuko Uchikawa Graziano
The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
Revista Latino-Americana de Enfermagem
Resíduos Orgánicos
Instrumentos Quirúrgicos
Mano
Guantes Protectores
Toxicidad;
title The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
title_full The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
title_fullStr The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
title_full_unstemmed The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
title_short The impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
title_sort impact of the use of different types of gloves and bare hands for preparation of clean surgical instruments
topic Resíduos Orgánicos
Instrumentos Quirúrgicos
Mano
Guantes Protectores
Toxicidad;
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-11692016000100417&lng=en&tlng=en
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