Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum
The current study aimed to examine the effect of steroid-free bovine follicular fluid (SFBFF) antiserum on ovarian immunohistochemical expression levels of 17β Estradiol in cyclic virgin female rats. After estrus synchronization, 80 adult virgin female Wistar rats (aged 60 days and weighed 156±4.82...
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine
2018-12-01
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| Series: | Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| Online Access: | http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/502 |
| _version_ | 1819052544159121408 |
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| author | Al-Sa'aidi, J.A. A Al-Jayashi. G. S. M Khafaji, S. S. |
| author_facet | Al-Sa'aidi, J.A. A Al-Jayashi. G. S. M Khafaji, S. S. |
| author_sort | Al-Sa'aidi, J.A. A |
| collection | DOAJ |
| description | The current study aimed to examine the effect of steroid-free bovine follicular fluid (SFBFF) antiserum on ovarian immunohistochemical expression levels of 17β Estradiol in cyclic virgin female rats. After estrus synchronization, 80 adult virgin female Wistar rats (aged 60 days and weighed 156±4.82 g) were randomly assigned into two equal groups (40 females each) and injected intrperitoneally, at late metestrous, with 100 µl of normal saline (control) and 100 µl of SFBFF antiserum (AI-SFBFF group). At each phase of the estrus cycle, 10 females from each group were anesthetized (by injection of 0.4 ml of thiopental sod./ animal), dissected and the ovaries were obtained for determination of immunohistochemical expression density of 17β Estradiol. Ovarian examination of AI-SFBFF group females demonstrated slight increase of estradiol immunohistochemical density at proestrus and mark increase at estrus and metestrus phases. It could be concluded that immunoneutralization of endogenous inhibin by SFBFF antiserum results in high levels of estradiol actions in reproductive organs. |
| first_indexed | 2024-12-21T12:21:31Z |
| format | Article |
| id | doaj.art-b19cb026e5724fef9551043206aad85a |
| institution | Directory Open Access Journal |
| issn | 1818-5746 2313-4429 |
| language | English |
| last_indexed | 2024-12-21T12:21:31Z |
| publishDate | 2018-12-01 |
| publisher | College of Veterinary Medicine |
| record_format | Article |
| series | Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| spelling | doaj.art-b19cb026e5724fef9551043206aad85a2022-12-21T19:04:17ZengCollege of Veterinary MedicineAl-Qadisiyah Journal of Veterinary Medicine Sciences1818-57462313-44292018-12-01172354210.29079/vol17iss2art502Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserumAl-Sa'aidi, J.A. A0Al-Jayashi. G. S. M1Khafaji, S. S.2Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Al-Qadisiyah, IraqDepartment of Physiology & Pharmacology, College of Veterinary Medicine, University of Al-Qadisiyah, IraqDepartment of Animal Production, College of Agriculture, University of Karbala, IraqThe current study aimed to examine the effect of steroid-free bovine follicular fluid (SFBFF) antiserum on ovarian immunohistochemical expression levels of 17β Estradiol in cyclic virgin female rats. After estrus synchronization, 80 adult virgin female Wistar rats (aged 60 days and weighed 156±4.82 g) were randomly assigned into two equal groups (40 females each) and injected intrperitoneally, at late metestrous, with 100 µl of normal saline (control) and 100 µl of SFBFF antiserum (AI-SFBFF group). At each phase of the estrus cycle, 10 females from each group were anesthetized (by injection of 0.4 ml of thiopental sod./ animal), dissected and the ovaries were obtained for determination of immunohistochemical expression density of 17β Estradiol. Ovarian examination of AI-SFBFF group females demonstrated slight increase of estradiol immunohistochemical density at proestrus and mark increase at estrus and metestrus phases. It could be concluded that immunoneutralization of endogenous inhibin by SFBFF antiserum results in high levels of estradiol actions in reproductive organs.http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/502 |
| spellingShingle | Al-Sa'aidi, J.A. A Al-Jayashi. G. S. M Khafaji, S. S. Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum Al-Qadisiyah Journal of Veterinary Medicine Sciences |
| title | Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum |
| title_full | Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum |
| title_fullStr | Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum |
| title_full_unstemmed | Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum |
| title_short | Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum |
| title_sort | ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum |
| url | http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/502 |
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