Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish
Sarcopenia is a common skeletal muscle degenerative disease characterized by decreased skeletal muscle mass and mitochondrial dysfunction that involves microRNAs (miR) as regulatory factors in various pathways. Exercise reduces age-related oxidative damage and chronic inflammation and increases auto...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Experimental Gerontology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0531556523001869 |
| _version_ | 1797740872487403520 |
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| author | Zhang-Lin Chen Cheng Guo Yun-Yi Zou Chen Feng Di-Xuan Yang Chen-Chen Sun Wei Wen Zhen-Jie Jian Zhe Zhao Qin Xiao Lan Zheng Xi-Yang Peng Zuo-Qiong Zhou Chang-Fa Tang |
| author_facet | Zhang-Lin Chen Cheng Guo Yun-Yi Zou Chen Feng Di-Xuan Yang Chen-Chen Sun Wei Wen Zhen-Jie Jian Zhe Zhao Qin Xiao Lan Zheng Xi-Yang Peng Zuo-Qiong Zhou Chang-Fa Tang |
| author_sort | Zhang-Lin Chen |
| collection | DOAJ |
| description | Sarcopenia is a common skeletal muscle degenerative disease characterized by decreased skeletal muscle mass and mitochondrial dysfunction that involves microRNAs (miR) as regulatory factors in various pathways. Exercise reduces age-related oxidative damage and chronic inflammation and increases autophagy, among others. Moreover, whether aerobic exercise can regulate mitochondrial homeostasis by modulating the miR-128/insulin-like growth factor-1 (IGF-1) signaling pathway and can improve sarcopenia requires further investigation. Interestingly, zebrafish have been used as a model for aging research for over a decade due to their many outstanding advantages. Therefore, we established a model of zebrafish sarcopenia using d-galactose immersion and observed substantial changes, including reduced skeletal muscle cross-sectional area, increased tissue fibrosis, decreased motility, increased skeletal muscle reactive oxygen species, and notable alterations in mitochondrial morphology and function. We found that miR-128 expression was considerably upregulated, where as Igf1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha were significantly downregulated; moreover, mitochondrial homeostasis was reduced. Four weeks of aerobic exercise delayed sarcopenia progression and prevented the disruption of mitochondrial function and homeostasis. The genes related to atrophy and miR-128 were downregulated, Igf1 expression was considerably upregulated, and the phosphorylation levels of Pi3k, Akt, and Foxo3a were upregulated. Furthermore, mitochondrial respiration and homeostasis were enhanced. In conclusion, aerobic exercise improved skeletal muscle quality and function via the miR-128/IGF-1 signaling pathway, consequently ameliorating mitochondrial homeostasis in aging skeletal muscle. |
| first_indexed | 2024-03-12T14:18:46Z |
| format | Article |
| id | doaj.art-b1a089e74f9d4dfa8a7d98e2f2724d46 |
| institution | Directory Open Access Journal |
| issn | 1873-6815 |
| language | English |
| last_indexed | 2024-03-12T14:18:46Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Experimental Gerontology |
| spelling | doaj.art-b1a089e74f9d4dfa8a7d98e2f2724d462023-08-20T04:37:27ZengElsevierExperimental Gerontology1873-68152023-09-01180112265Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafishZhang-Lin Chen0Cheng Guo1Yun-Yi Zou2Chen Feng3Di-Xuan Yang4Chen-Chen Sun5Wei Wen6Zhen-Jie Jian7Zhe Zhao8Qin Xiao9Lan Zheng10Xi-Yang Peng11Zuo-Qiong Zhou12Chang-Fa Tang13Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaCorresponding authors.; Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaCorresponding authors.; Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaCorresponding authors.; Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, College of Physical Education, Hunan Normal University, 410012 Changsha, ChinaSarcopenia is a common skeletal muscle degenerative disease characterized by decreased skeletal muscle mass and mitochondrial dysfunction that involves microRNAs (miR) as regulatory factors in various pathways. Exercise reduces age-related oxidative damage and chronic inflammation and increases autophagy, among others. Moreover, whether aerobic exercise can regulate mitochondrial homeostasis by modulating the miR-128/insulin-like growth factor-1 (IGF-1) signaling pathway and can improve sarcopenia requires further investigation. Interestingly, zebrafish have been used as a model for aging research for over a decade due to their many outstanding advantages. Therefore, we established a model of zebrafish sarcopenia using d-galactose immersion and observed substantial changes, including reduced skeletal muscle cross-sectional area, increased tissue fibrosis, decreased motility, increased skeletal muscle reactive oxygen species, and notable alterations in mitochondrial morphology and function. We found that miR-128 expression was considerably upregulated, where as Igf1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha were significantly downregulated; moreover, mitochondrial homeostasis was reduced. Four weeks of aerobic exercise delayed sarcopenia progression and prevented the disruption of mitochondrial function and homeostasis. The genes related to atrophy and miR-128 were downregulated, Igf1 expression was considerably upregulated, and the phosphorylation levels of Pi3k, Akt, and Foxo3a were upregulated. Furthermore, mitochondrial respiration and homeostasis were enhanced. In conclusion, aerobic exercise improved skeletal muscle quality and function via the miR-128/IGF-1 signaling pathway, consequently ameliorating mitochondrial homeostasis in aging skeletal muscle.http://www.sciencedirect.com/science/article/pii/S0531556523001869Aerobic exerciseZebrafishSarcopeniaMitochondrial homeostasisd-Galactose |
| spellingShingle | Zhang-Lin Chen Cheng Guo Yun-Yi Zou Chen Feng Di-Xuan Yang Chen-Chen Sun Wei Wen Zhen-Jie Jian Zhe Zhao Qin Xiao Lan Zheng Xi-Yang Peng Zuo-Qiong Zhou Chang-Fa Tang Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish Experimental Gerontology Aerobic exercise Zebrafish Sarcopenia Mitochondrial homeostasis d-Galactose |
| title | Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish |
| title_full | Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish |
| title_fullStr | Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish |
| title_full_unstemmed | Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish |
| title_short | Aerobic exercise enhances mitochondrial homeostasis to counteract D-galactose-induced sarcopenia in zebrafish |
| title_sort | aerobic exercise enhances mitochondrial homeostasis to counteract d galactose induced sarcopenia in zebrafish |
| topic | Aerobic exercise Zebrafish Sarcopenia Mitochondrial homeostasis d-Galactose |
| url | http://www.sciencedirect.com/science/article/pii/S0531556523001869 |
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