Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis
Sandrine Blanchet-Réthoré,1 Valérie Bourdès,1 Annick Mercenier,2 Cyrille H Haddar,3 Paul O Verhoeven,3 Philippe Andres4 1CUTIS (Clinical Unit for Tests and Imaging of Skin), Evaluation Department, Nestlé Skin Health/Galderma Research and Develop...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-07-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
| Subjects: | |
| Online Access: | https://www.dovepress.com/effect-of-a-lotion-containing-the-heat-treated-probiotic-strain-lactob-peer-reviewed-article-CCID |
| _version_ | 1811239855240249344 |
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| author | Blanchet-Réthoré S Bourdès V Mercenier A Haddar CH Verhoeven PO Andres P |
| author_facet | Blanchet-Réthoré S Bourdès V Mercenier A Haddar CH Verhoeven PO Andres P |
| author_sort | Blanchet-Réthoré S |
| collection | DOAJ |
| description | Sandrine Blanchet-Réthoré,1 Valérie Bourdès,1 Annick Mercenier,2 Cyrille H Haddar,3 Paul O Verhoeven,3 Philippe Andres4 1CUTIS (Clinical Unit for Tests and Imaging of Skin), Evaluation Department, Nestlé Skin Health/Galderma Research and Development, Sophia-Antipolis, France; ²Host Microbiome Interaction Group, Gut Ecosystem Department, Institute of Nutritional Science, Nestlé Research Center, Lausanne, Switzerland; 3Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne and GIMAP (Mucosal Immunity and Pathogen Agents Group), Saint-Etienne, France; 4Medical Affairs Department, Galderma International, Paris, France Objective: Staphylococcus aureus dominates the skin microbiota in patients with atopic dermatitis (AD), with bacterial loads correlating with disease severity. The aim of this exploratory study was to investigate the effect of a cosmetic lotion containing heat-treated Lactobacillus johnsonii NCC 533 (HT La1) on S. aureus colonization in AD patients. Methods: This open-label, multicenter study was performed in AD patients in Germany. First, detection of S. aureus was performed in all patients using the swab or scrub-wash method of sampling, followed by quantitative culture or quantitative polymerase chain reaction. Repeatability and reproducibility of all method combinations were evaluated to select the best combination of sampling and quantification. Second, a lotion containing HT La1 was applied to lesional skin twice daily for 3 weeks. Scoring using local objective SCORing Atopic Dermatitis (SCORAD), measurement of S. aureus load, and lesional microbiome analysis were performed before and after the 3-week treatment period. Results: Thirty-one patients with AD were included in the study. All sampling and quantification methods were found to be robust, reproducible, and repeatable for assessing S. aureus load. For simplicity, a combination of swab and quantitative polymerase chain reaction was chosen to assess the efficacy of HT La1. Following application of a lotion containing HT La1 to AD lesions for 3 weeks, a reduction in S. aureus load was observed in patients, which correlated with a decrease in local objective SCORAD. Interestingly, high baseline skin concentrations of S. aureus were associated with good responses to the lotion. Conclusion: This study demonstrated that the application of a lotion containing HT La1 to the lesional skin of patients with AD for 3 weeks controlled S. aureus colonization and was associated with local clinical improvement (SCORAD). These findings support further development of topical treatments containing heat-treated nonreplicating beneficial bacteria for patients with AD. Keywords: atopic dermatitis, S. aureus, heat treated La1, HT La1, L. johnsonii NCC 533, microbiology |
| first_indexed | 2024-04-12T13:08:31Z |
| format | Article |
| id | doaj.art-b1a1d778efa8477a82d454174f8e5ee6 |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2024-04-12T13:08:31Z |
| publishDate | 2017-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-b1a1d778efa8477a82d454174f8e5ee62022-12-22T03:31:56ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152017-07-01Volume 1024925733561Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitisBlanchet-Réthoré SBourdès VMercenier AHaddar CHVerhoeven POAndres PSandrine Blanchet-Réthoré,1 Valérie Bourdès,1 Annick Mercenier,2 Cyrille H Haddar,3 Paul O Verhoeven,3 Philippe Andres4 1CUTIS (Clinical Unit for Tests and Imaging of Skin), Evaluation Department, Nestlé Skin Health/Galderma Research and Development, Sophia-Antipolis, France; ²Host Microbiome Interaction Group, Gut Ecosystem Department, Institute of Nutritional Science, Nestlé Research Center, Lausanne, Switzerland; 3Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne and GIMAP (Mucosal Immunity and Pathogen Agents Group), Saint-Etienne, France; 4Medical Affairs Department, Galderma International, Paris, France Objective: Staphylococcus aureus dominates the skin microbiota in patients with atopic dermatitis (AD), with bacterial loads correlating with disease severity. The aim of this exploratory study was to investigate the effect of a cosmetic lotion containing heat-treated Lactobacillus johnsonii NCC 533 (HT La1) on S. aureus colonization in AD patients. Methods: This open-label, multicenter study was performed in AD patients in Germany. First, detection of S. aureus was performed in all patients using the swab or scrub-wash method of sampling, followed by quantitative culture or quantitative polymerase chain reaction. Repeatability and reproducibility of all method combinations were evaluated to select the best combination of sampling and quantification. Second, a lotion containing HT La1 was applied to lesional skin twice daily for 3 weeks. Scoring using local objective SCORing Atopic Dermatitis (SCORAD), measurement of S. aureus load, and lesional microbiome analysis were performed before and after the 3-week treatment period. Results: Thirty-one patients with AD were included in the study. All sampling and quantification methods were found to be robust, reproducible, and repeatable for assessing S. aureus load. For simplicity, a combination of swab and quantitative polymerase chain reaction was chosen to assess the efficacy of HT La1. Following application of a lotion containing HT La1 to AD lesions for 3 weeks, a reduction in S. aureus load was observed in patients, which correlated with a decrease in local objective SCORAD. Interestingly, high baseline skin concentrations of S. aureus were associated with good responses to the lotion. Conclusion: This study demonstrated that the application of a lotion containing HT La1 to the lesional skin of patients with AD for 3 weeks controlled S. aureus colonization and was associated with local clinical improvement (SCORAD). These findings support further development of topical treatments containing heat-treated nonreplicating beneficial bacteria for patients with AD. Keywords: atopic dermatitis, S. aureus, heat treated La1, HT La1, L. johnsonii NCC 533, microbiologyhttps://www.dovepress.com/effect-of-a-lotion-containing-the-heat-treated-probiotic-strain-lactob-peer-reviewed-article-CCIDAtopic DermatitisS. aureusHeat treated La1HT La1L. johnsonii NCC 533Microbiology |
| spellingShingle | Blanchet-Réthoré S Bourdès V Mercenier A Haddar CH Verhoeven PO Andres P Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis Clinical, Cosmetic and Investigational Dermatology Atopic Dermatitis S. aureus Heat treated La1 HT La1 L. johnsonii NCC 533 Microbiology |
| title | Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis |
| title_full | Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis |
| title_fullStr | Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis |
| title_full_unstemmed | Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis |
| title_short | Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis |
| title_sort | effect of a lotion containing the heat treated probiotic strain lactobacillus johnsonii ncc 533 on staphylococcus aureus colonization in atopic dermatitis |
| topic | Atopic Dermatitis S. aureus Heat treated La1 HT La1 L. johnsonii NCC 533 Microbiology |
| url | https://www.dovepress.com/effect-of-a-lotion-containing-the-heat-treated-probiotic-strain-lactob-peer-reviewed-article-CCID |
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