Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids
The development of a biotechnological method for the production of new biologically active phosphatidylcholine containing monoterpene citronellic acid (CA) was the aim of this work. Incorporation of citronellic acid (CA) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed acidolysis proce...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/2/314 |
| _version_ | 1811326226145476608 |
|---|---|
| author | Magdalena Rychlicka Natalia Niezgoda Anna Gliszczyńska |
| author_facet | Magdalena Rychlicka Natalia Niezgoda Anna Gliszczyńska |
| author_sort | Magdalena Rychlicka |
| collection | DOAJ |
| description | The development of a biotechnological method for the production of new biologically active phosphatidylcholine containing monoterpene citronellic acid (CA) was the aim of this work. Incorporation of citronellic acid (CA) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed acidolysis process was studied. Isoprenoid acid CA was used as an acyl donor and five commercially available immobilized lipases were examined as biocatalysts. The effects of organic solvent, enzyme load, reaction time and molar ratio of substrates on the incorporation of citronellic acid (CA) into the phospholipids were evaluated. Modified phospholipid fraction enriched with CA in the sn-1 position (39% of incorporation) was obtained in high 33% yield using Novozym 435 as biocatalyst. In this study a biotechnological method for production of new phospholipid biopreparation enriched with citronellic acid, which can play an important role as a nutraceutical, was applied. |
| first_indexed | 2024-04-13T14:46:24Z |
| format | Article |
| id | doaj.art-b1a2be5e755c4845bde9d36d7e818c6a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T14:46:24Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b1a2be5e755c4845bde9d36d7e818c6a2022-12-22T02:42:44ZengMDPI AGMolecules1420-30492018-02-0123231410.3390/molecules23020314molecules23020314Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-PhospholipidsMagdalena Rychlicka0Natalia Niezgoda1Anna Gliszczyńska2Department of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50–375 Wrocław, PolandDepartment of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50–375 Wrocław, PolandDepartment of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50–375 Wrocław, PolandThe development of a biotechnological method for the production of new biologically active phosphatidylcholine containing monoterpene citronellic acid (CA) was the aim of this work. Incorporation of citronellic acid (CA) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed acidolysis process was studied. Isoprenoid acid CA was used as an acyl donor and five commercially available immobilized lipases were examined as biocatalysts. The effects of organic solvent, enzyme load, reaction time and molar ratio of substrates on the incorporation of citronellic acid (CA) into the phospholipids were evaluated. Modified phospholipid fraction enriched with CA in the sn-1 position (39% of incorporation) was obtained in high 33% yield using Novozym 435 as biocatalyst. In this study a biotechnological method for production of new phospholipid biopreparation enriched with citronellic acid, which can play an important role as a nutraceutical, was applied.http://www.mdpi.com/1420-3049/23/2/314acidolysisegg-yolk phosphatidylcholinecitronellic acidisoprenoidslipaseanticancer activity |
| spellingShingle | Magdalena Rychlicka Natalia Niezgoda Anna Gliszczyńska Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids Molecules acidolysis egg-yolk phosphatidylcholine citronellic acid isoprenoids lipase anticancer activity |
| title | Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids |
| title_full | Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids |
| title_fullStr | Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids |
| title_full_unstemmed | Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids |
| title_short | Lipase-Catalyzed Acidolysis of Egg-Yolk Phosphatidylcholine with Citronellic Acid. New Insight into Synthesis of Isoprenoid-Phospholipids |
| title_sort | lipase catalyzed acidolysis of egg yolk phosphatidylcholine with citronellic acid new insight into synthesis of isoprenoid phospholipids |
| topic | acidolysis egg-yolk phosphatidylcholine citronellic acid isoprenoids lipase anticancer activity |
| url | http://www.mdpi.com/1420-3049/23/2/314 |
| work_keys_str_mv | AT magdalenarychlicka lipasecatalyzedacidolysisofeggyolkphosphatidylcholinewithcitronellicacidnewinsightintosynthesisofisoprenoidphospholipids AT natalianiezgoda lipasecatalyzedacidolysisofeggyolkphosphatidylcholinewithcitronellicacidnewinsightintosynthesisofisoprenoidphospholipids AT annagliszczynska lipasecatalyzedacidolysisofeggyolkphosphatidylcholinewithcitronellicacidnewinsightintosynthesisofisoprenoidphospholipids |