Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing
Despite the widespread application of decentralized wastewater treatment (WWT) facilities in China, relatively few research has used the multi-media biological filter (MMBF) facilities to investigate the microorganism characteristics. This study utilizes 16S rRNA high-throughput sequencing (HTS) tec...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242506/full |
| _version_ | 1827818486568583168 |
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| author | Shanqian Huang Yaping Kong Yao Chen Xuewen Huang Pengfei Ma Xuexin Liu |
| author_facet | Shanqian Huang Yaping Kong Yao Chen Xuewen Huang Pengfei Ma Xuexin Liu |
| author_sort | Shanqian Huang |
| collection | DOAJ |
| description | Despite the widespread application of decentralized wastewater treatment (WWT) facilities in China, relatively few research has used the multi-media biological filter (MMBF) facilities to investigate the microorganism characteristics. This study utilizes 16S rRNA high-throughput sequencing (HTS) technology to examine the microbial biodiversity of a representative wastewater treatment (WWT) system in an expressway service area. The pathways of nitrogen removal along the treatment route were analyzed in conjunction with water quality monitoring. The distribution and composition of microbial flora in the samples were examined, and the dominant flora were identified using LEfSe analysis. The FAPROTAX methodology was employed to investigate the relative abundance of genes associated with the nitrogen cycle and to discern the presence of functional genes involved in nitrogen metabolism. On average, the method has a high level of efficiency in removing COD, TN, NH3-N, and TP from the effluent. The analysis of the microbial community identified a total of 40 phyla, 111 classes, 143 orders, 263 families, and 419 genera. The phyla that were predominantly observed include Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, Nitrospirae, Bacteroidetes. The results show that the system has achieved high performance in nitrogen removal, the abundance of nitrification genes is significantly higher than that of other nitrogen cycle genes such as denitrification, and there are six nitrogen metabolism pathways, primarily nitrification, among which Nitrospirae and Nitrospira are the core differentiated flora that can adapt to low temperature conditions and participate in nitrification, and are the dominant nitrogen removal flora in cold regions. This work aims to comprehensively investigate the diversity and functional properties of the bacterial community in decentralized WWT processes. |
| first_indexed | 2024-03-12T00:55:27Z |
| format | Article |
| id | doaj.art-b1a438012e804c33aa4c5e82156f1639 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T00:55:27Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b1a438012e804c33aa4c5e82156f16392023-09-14T13:50:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12425061242506Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencingShanqian Huang0Yaping Kong1Yao Chen2Xuewen Huang3Pengfei Ma4Xuexin Liu5Center of Environment Protection, China Academy of Transportation Sciences, Beijing, ChinaCenter of Environment Protection, China Academy of Transportation Sciences, Beijing, ChinaCenter of Environment Protection, China Academy of Transportation Sciences, Beijing, ChinaAnhui Transportation Holding Group CO., LTD., Hefei, ChinaQinghai Expressway Maintenance Service CO., LTD., Xining, ChinaCenter of Environment Protection, China Academy of Transportation Sciences, Beijing, ChinaDespite the widespread application of decentralized wastewater treatment (WWT) facilities in China, relatively few research has used the multi-media biological filter (MMBF) facilities to investigate the microorganism characteristics. This study utilizes 16S rRNA high-throughput sequencing (HTS) technology to examine the microbial biodiversity of a representative wastewater treatment (WWT) system in an expressway service area. The pathways of nitrogen removal along the treatment route were analyzed in conjunction with water quality monitoring. The distribution and composition of microbial flora in the samples were examined, and the dominant flora were identified using LEfSe analysis. The FAPROTAX methodology was employed to investigate the relative abundance of genes associated with the nitrogen cycle and to discern the presence of functional genes involved in nitrogen metabolism. On average, the method has a high level of efficiency in removing COD, TN, NH3-N, and TP from the effluent. The analysis of the microbial community identified a total of 40 phyla, 111 classes, 143 orders, 263 families, and 419 genera. The phyla that were predominantly observed include Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, Nitrospirae, Bacteroidetes. The results show that the system has achieved high performance in nitrogen removal, the abundance of nitrification genes is significantly higher than that of other nitrogen cycle genes such as denitrification, and there are six nitrogen metabolism pathways, primarily nitrification, among which Nitrospirae and Nitrospira are the core differentiated flora that can adapt to low temperature conditions and participate in nitrification, and are the dominant nitrogen removal flora in cold regions. This work aims to comprehensively investigate the diversity and functional properties of the bacterial community in decentralized WWT processes.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242506/full16S rRNAmulti-media biofilterdecentralized wastewater treatmentdenitrification floraLEfSeFAPROTAX |
| spellingShingle | Shanqian Huang Yaping Kong Yao Chen Xuewen Huang Pengfei Ma Xuexin Liu Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing Frontiers in Microbiology 16S rRNA multi-media biofilter decentralized wastewater treatment denitrification flora LEfSe FAPROTAX |
| title | Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing |
| title_full | Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing |
| title_fullStr | Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing |
| title_full_unstemmed | Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing |
| title_short | Microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16S rRNA sequencing |
| title_sort | microbial denitrification characteristics of typical decentralized wastewater treatment processes based on 16s rrna sequencing |
| topic | 16S rRNA multi-media biofilter decentralized wastewater treatment denitrification flora LEfSe FAPROTAX |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242506/full |
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