Human HMGB1 does not induce eryptosis <i>in vitro</i>
<b>Aim:</b> To study the ability of human high mobility group box protein 1 (HMGB1) to induce eryptosis <i>in vitro</i>.<br /> <b>Material and methods:</b> Blood collected from six healthy volunteers was incubated with HMGB1 (0-0.2-1-5 ng per ml). Eryptosis...
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| Format: | Article |
| Language: | English |
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National Scientific Medical Center
2022-04-01
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| Series: | Ķazaķstannyṇ Klinikalyķ Medicinasy |
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| Online Access: | https://www.clinmedkaz.org/download/human-hmgb1-does-not-induce-eryptosis-in-vitro-11934.pdf |
| _version_ | 1828806714587086848 |
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| author | Maryna Tkachenko Anatolii Onishchenko Dmytro Butov Tetyana Butova Anton Tkachenko |
| author_facet | Maryna Tkachenko Anatolii Onishchenko Dmytro Butov Tetyana Butova Anton Tkachenko |
| author_sort | Maryna Tkachenko |
| collection | DOAJ |
| description | <b>Aim:</b> To study the ability of human high mobility group box protein 1 (HMGB1) to induce eryptosis <i>in vitro</i>.<br />
<b>Material and methods:</b> Blood collected from six healthy volunteers was incubated with HMGB1 (0-0.2-1-5 ng per ml). Eryptosis of red blood cells was assessed by Annexin V staining and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining by flow cytometry. The forward scatter (FSC) fluorescence was used to evaluate the morphology of red blood cells.<br />
<b>Results:</b> Exposure of erythrocytes to HMGB1 did not affect the morphology of erythrocytes, evidenced by no changes in the percentage of cells with small volume, i.e. shrunken cells, and erythrocytes with large volume, i.e. enlarged cells. HMGB1 had no impact on phosphatidylserine externalization, which is confirmed by the absence of statistically significant changes in the amount of phosphatidylserine-displaying cells and the mean fluorescence intensity (MFI) values of Annexin V-FITC in cells exposed to different concentrations of HMGB1. Furthermore, H2DCFDA staining revealed that the HMGB1 did not induce oxidative stress.<br />
<b>Conclusion</b>: HMGB1 does not promote eryptosis of human erythrocytes at concentrations of up to 5 ng/ml.<br />
<b>Key words: </b>high mobility group box protein 1, erythrocytes, inflammation, cell death |
| first_indexed | 2024-12-12T08:14:37Z |
| format | Article |
| id | doaj.art-b1a54c1e09ce43dbb53a2f858fef9442 |
| institution | Directory Open Access Journal |
| issn | 1812-2892 2313-1519 |
| language | English |
| last_indexed | 2024-12-12T08:14:37Z |
| publishDate | 2022-04-01 |
| publisher | National Scientific Medical Center |
| record_format | Article |
| series | Ķazaķstannyṇ Klinikalyķ Medicinasy |
| spelling | doaj.art-b1a54c1e09ce43dbb53a2f858fef94422022-12-22T00:31:40ZengNational Scientific Medical CenterĶazaķstannyṇ Klinikalyķ Medicinasy1812-28922313-15192022-04-01192333710.23950/jcmk/11934Human HMGB1 does not induce eryptosis <i>in vitro</i>Maryna Tkachenko0Anatolii Onishchenko1Dmytro Butov2Tetyana Butova3Anton Tkachenko4Department of Internal Medicine No. 2, Clinical Immunology and Allergology named after academician L.T. Malaya, Kharkiv National Medical University, Kharkiv, Ukraine; Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, UkraineResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, UkraineDepartment of Phthisiology and Pulmonology, Kharkiv National Medical University, Kharkiv, UkraineResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, UkraineResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, Ukraine<b>Aim:</b> To study the ability of human high mobility group box protein 1 (HMGB1) to induce eryptosis <i>in vitro</i>.<br /> <b>Material and methods:</b> Blood collected from six healthy volunteers was incubated with HMGB1 (0-0.2-1-5 ng per ml). Eryptosis of red blood cells was assessed by Annexin V staining and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining by flow cytometry. The forward scatter (FSC) fluorescence was used to evaluate the morphology of red blood cells.<br /> <b>Results:</b> Exposure of erythrocytes to HMGB1 did not affect the morphology of erythrocytes, evidenced by no changes in the percentage of cells with small volume, i.e. shrunken cells, and erythrocytes with large volume, i.e. enlarged cells. HMGB1 had no impact on phosphatidylserine externalization, which is confirmed by the absence of statistically significant changes in the amount of phosphatidylserine-displaying cells and the mean fluorescence intensity (MFI) values of Annexin V-FITC in cells exposed to different concentrations of HMGB1. Furthermore, H2DCFDA staining revealed that the HMGB1 did not induce oxidative stress.<br /> <b>Conclusion</b>: HMGB1 does not promote eryptosis of human erythrocytes at concentrations of up to 5 ng/ml.<br /> <b>Key words: </b>high mobility group box protein 1, erythrocytes, inflammation, cell deathhttps://www.clinmedkaz.org/download/human-hmgb1-does-not-induce-eryptosis-in-vitro-11934.pdfhigh mobility group box protein 1erythrocytesinflammationcell death |
| spellingShingle | Maryna Tkachenko Anatolii Onishchenko Dmytro Butov Tetyana Butova Anton Tkachenko Human HMGB1 does not induce eryptosis <i>in vitro</i> Ķazaķstannyṇ Klinikalyķ Medicinasy high mobility group box protein 1 erythrocytes inflammation cell death |
| title | Human HMGB1 does not induce eryptosis <i>in vitro</i> |
| title_full | Human HMGB1 does not induce eryptosis <i>in vitro</i> |
| title_fullStr | Human HMGB1 does not induce eryptosis <i>in vitro</i> |
| title_full_unstemmed | Human HMGB1 does not induce eryptosis <i>in vitro</i> |
| title_short | Human HMGB1 does not induce eryptosis <i>in vitro</i> |
| title_sort | human hmgb1 does not induce eryptosis i in vitro i |
| topic | high mobility group box protein 1 erythrocytes inflammation cell death |
| url | https://www.clinmedkaz.org/download/human-hmgb1-does-not-induce-eryptosis-in-vitro-11934.pdf |
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