Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method
Abstract In the present study, conditions for Bacillus mucilaginous fermentation using Agaricus bisporus wastewater as culture medium were optimized. We analyzed the total number of living B. mucilaginous in the fermentation broth using multispectral imaging flow cytometry. Single-factor experiments...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-08-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0671-7 |
| _version_ | 1818573179032961024 |
|---|---|
| author | Jiafu Huang Yixin Ou Danfeng Zhang Guoguang Zhang Yutian Pan |
| author_facet | Jiafu Huang Yixin Ou Danfeng Zhang Guoguang Zhang Yutian Pan |
| author_sort | Jiafu Huang |
| collection | DOAJ |
| description | Abstract In the present study, conditions for Bacillus mucilaginous fermentation using Agaricus bisporus wastewater as culture medium were optimized. We analyzed the total number of living B. mucilaginous in the fermentation broth using multispectral imaging flow cytometry. Single-factor experiments were carried out, where a Plackett–Burman design was used to screen out three factors from the original six factors of processing wastewater solubility, initial pH, inoculum size, liquid volume, culture temperature, and rotation speed that affected the total number of viable B. mucilaginous. The Box–Behnken response surface method was used to optimize interactions between the three main factors and predict optimal fermentation conditions. Factors significantly affecting the total number of viable B. mucilaginous, including shaking speed, culturing temperature, and initial pH, were investigated. The optimum conditions for B. mucilaginous fermentation in A. bisporus wastewater were a rotational speed of 195 rpm, culture temperature of 29 °C, initial pH of 6.5, solubility of 0.5%, 8% inoculation volume, and 90 mL liquid volume in a 250 mL flask, culture time of 48 h. Under these conditions, the concentration of total viable bacteria reached 2.16 ± 0.02 × 108 Obj/mL, which meets the national standard. A. bisporus wastewater can be used for the cultivation of B. mucilaginous. |
| first_indexed | 2024-12-15T00:07:36Z |
| format | Article |
| id | doaj.art-b1a8da4052f9401eb948deb7595fe8f0 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-15T00:07:36Z |
| publishDate | 2018-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-b1a8da4052f9401eb948deb7595fe8f02022-12-21T22:42:41ZengSpringerOpenAMB Express2191-08552018-08-018111210.1186/s13568-018-0671-7Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface methodJiafu Huang0Yixin Ou1Danfeng Zhang2Guoguang Zhang3Yutian Pan4Engineering Technological Center of Mushroom Industry, Minnan Normal UniversityEngineering Technological Center of Mushroom Industry, Minnan Normal UniversityEngineering Technological Center of Mushroom Industry, Minnan Normal UniversityEngineering Technological Center of Mushroom Industry, Minnan Normal UniversityEngineering Technological Center of Mushroom Industry, Minnan Normal UniversityAbstract In the present study, conditions for Bacillus mucilaginous fermentation using Agaricus bisporus wastewater as culture medium were optimized. We analyzed the total number of living B. mucilaginous in the fermentation broth using multispectral imaging flow cytometry. Single-factor experiments were carried out, where a Plackett–Burman design was used to screen out three factors from the original six factors of processing wastewater solubility, initial pH, inoculum size, liquid volume, culture temperature, and rotation speed that affected the total number of viable B. mucilaginous. The Box–Behnken response surface method was used to optimize interactions between the three main factors and predict optimal fermentation conditions. Factors significantly affecting the total number of viable B. mucilaginous, including shaking speed, culturing temperature, and initial pH, were investigated. The optimum conditions for B. mucilaginous fermentation in A. bisporus wastewater were a rotational speed of 195 rpm, culture temperature of 29 °C, initial pH of 6.5, solubility of 0.5%, 8% inoculation volume, and 90 mL liquid volume in a 250 mL flask, culture time of 48 h. Under these conditions, the concentration of total viable bacteria reached 2.16 ± 0.02 × 108 Obj/mL, which meets the national standard. A. bisporus wastewater can be used for the cultivation of B. mucilaginous.http://link.springer.com/article/10.1186/s13568-018-0671-7Agaricus bisporus wastewaterBacillus mucilaginousMultispectral imaging flow cytometryPlackett–BurmanBox–Behnken response surface |
| spellingShingle | Jiafu Huang Yixin Ou Danfeng Zhang Guoguang Zhang Yutian Pan Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method AMB Express Agaricus bisporus wastewater Bacillus mucilaginous Multispectral imaging flow cytometry Plackett–Burman Box–Behnken response surface |
| title | Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method |
| title_full | Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method |
| title_fullStr | Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method |
| title_full_unstemmed | Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method |
| title_short | Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method |
| title_sort | optimization of the culture condition of bacillus mucilaginous using agaricus bisporus industrial wastewater by plackett burman combined with box behnken response surface method |
| topic | Agaricus bisporus wastewater Bacillus mucilaginous Multispectral imaging flow cytometry Plackett–Burman Box–Behnken response surface |
| url | http://link.springer.com/article/10.1186/s13568-018-0671-7 |
| work_keys_str_mv | AT jiafuhuang optimizationofthecultureconditionofbacillusmucilaginoususingagaricusbisporusindustrialwastewaterbyplackettburmancombinedwithboxbehnkenresponsesurfacemethod AT yixinou optimizationofthecultureconditionofbacillusmucilaginoususingagaricusbisporusindustrialwastewaterbyplackettburmancombinedwithboxbehnkenresponsesurfacemethod AT danfengzhang optimizationofthecultureconditionofbacillusmucilaginoususingagaricusbisporusindustrialwastewaterbyplackettburmancombinedwithboxbehnkenresponsesurfacemethod AT guoguangzhang optimizationofthecultureconditionofbacillusmucilaginoususingagaricusbisporusindustrialwastewaterbyplackettburmancombinedwithboxbehnkenresponsesurfacemethod AT yutianpan optimizationofthecultureconditionofbacillusmucilaginoususingagaricusbisporusindustrialwastewaterbyplackettburmancombinedwithboxbehnkenresponsesurfacemethod |