Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE)
Childhood- onset systemic lupus erythematosus (cSLE) is a multisystem inflammatory disease that can lead to severe clinical conditions resulting in early comorbidities. Several genetic, environmental, and immunological factors are known to influence the onset of the disease. MiRNAs have been already...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-11-01
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| Series: | Autoimmunity |
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| Online Access: | http://dx.doi.org/10.1080/08916934.2022.2128781 |
| _version_ | 1797684624438067200 |
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| author | Denise de Queiroga Nascimento Isaura Isabelle Fonseca Gomes da Silva Camilla Albertina Dantas Lima André de Souza Cavalcanti Luciana Rodrigues Roberti Rosane Gomes de Paula Queiroz Virginia Paes Leme Ferriani Sergio Crovella Luciana Martins de Carvalho Paula Sandrin-Garcia |
| author_facet | Denise de Queiroga Nascimento Isaura Isabelle Fonseca Gomes da Silva Camilla Albertina Dantas Lima André de Souza Cavalcanti Luciana Rodrigues Roberti Rosane Gomes de Paula Queiroz Virginia Paes Leme Ferriani Sergio Crovella Luciana Martins de Carvalho Paula Sandrin-Garcia |
| author_sort | Denise de Queiroga Nascimento |
| collection | DOAJ |
| description | Childhood- onset systemic lupus erythematosus (cSLE) is a multisystem inflammatory disease that can lead to severe clinical conditions resulting in early comorbidities. Several genetic, environmental, and immunological factors are known to influence the onset of the disease. MiRNAs have been already considered as potential actors involved in the development and activity of the SLE. Thus, understanding the behavior of these regulators can contribute to clarify the inflammatory process affecting SLE patients. Among miRNAs, miR-125b-5p and miR-9-5p targeting NFKB1 and TRAF6 genes can be involved in the etio-pathogenesis of the disease by modulating inflammation. In this study we evaluated miR-9-5p and miR-125b-5p expression and its target genes NFKB1 and TRAF6 in peripheral blood samples (PBMC) from the 35 cSLE patients and 35 healthy controls. MiRNAs and gene target expression have been evaluated by using RT-PCR with specific TaqMan® probes. Both miR-9-5p [Fold Change (FC) = −2.21; p = 0.002] and miR-125b-5p (FC= −3.30; p < 0.0001) and NFKB1 (FC = −1.84; p < 0.001) were downregulated in cSLE patients, while TRAF6 was upregulated (FC = 1.80; p = 0.006) in cSLE patients when compared to controls. A significant correlation was found between miR-125b-5p and its target gene NFKB1 [Spearman (r) = 0.47; p = 0.023]. Our results showed miR-125b-5p and miR-9-5p differential expression in cSLE patients, possibly contributing to better understanding the role of these regulators in cSLE development and disease pathogenesis. |
| first_indexed | 2024-03-12T00:32:25Z |
| format | Article |
| id | doaj.art-b1a8f4212e324fbca6798eeb86cd51d9 |
| institution | Directory Open Access Journal |
| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:32:25Z |
| publishDate | 2022-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj.art-b1a8f4212e324fbca6798eeb86cd51d92023-09-15T10:12:26ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2022-11-0155851551910.1080/08916934.2022.21287812128781Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE)Denise de Queiroga Nascimento0Isaura Isabelle Fonseca Gomes da Silva1Camilla Albertina Dantas Lima2André de Souza Cavalcanti3Luciana Rodrigues Roberti4Rosane Gomes de Paula Queiroz5Virginia Paes Leme Ferriani6Sergio Crovella7Luciana Martins de Carvalho8Paula Sandrin-Garcia9Department of genetics, PostGraduate Program in Genetics, Federal University of Pernambuco, Recife, Pernambuco, BrazilDepartment of genetics, PostGraduate Program in Genetics, Federal University of Pernambuco, Recife, Pernambuco, BrazilLaboratory of Immunopathology Keizo AsamiDivision of Pediatric Rheumatology, Clinical Hospital of Federal University of PernambucoDivision of Pediatric Rheumatology, Clinic Hospital of Ribeirão Preto Medical School, University of São PauloDivision of Pediatric Rheumatology, Clinic Hospital of Ribeirão Preto Medical School, University of São PauloDivision of Pediatric Rheumatology, Clinic Hospital of Ribeirão Preto Medical School, University of São PauloDepartment of Biological and Environmental Sciences, Biological Sciences Program, College of Arts and Sciences, Qatar UniversityDivision of Pediatric Rheumatology, Clinic Hospital of Ribeirão Preto Medical School, University of São PauloDepartment of genetics, PostGraduate Program in Genetics, Federal University of Pernambuco, Recife, Pernambuco, BrazilChildhood- onset systemic lupus erythematosus (cSLE) is a multisystem inflammatory disease that can lead to severe clinical conditions resulting in early comorbidities. Several genetic, environmental, and immunological factors are known to influence the onset of the disease. MiRNAs have been already considered as potential actors involved in the development and activity of the SLE. Thus, understanding the behavior of these regulators can contribute to clarify the inflammatory process affecting SLE patients. Among miRNAs, miR-125b-5p and miR-9-5p targeting NFKB1 and TRAF6 genes can be involved in the etio-pathogenesis of the disease by modulating inflammation. In this study we evaluated miR-9-5p and miR-125b-5p expression and its target genes NFKB1 and TRAF6 in peripheral blood samples (PBMC) from the 35 cSLE patients and 35 healthy controls. MiRNAs and gene target expression have been evaluated by using RT-PCR with specific TaqMan® probes. Both miR-9-5p [Fold Change (FC) = −2.21; p = 0.002] and miR-125b-5p (FC= −3.30; p < 0.0001) and NFKB1 (FC = −1.84; p < 0.001) were downregulated in cSLE patients, while TRAF6 was upregulated (FC = 1.80; p = 0.006) in cSLE patients when compared to controls. A significant correlation was found between miR-125b-5p and its target gene NFKB1 [Spearman (r) = 0.47; p = 0.023]. Our results showed miR-125b-5p and miR-9-5p differential expression in cSLE patients, possibly contributing to better understanding the role of these regulators in cSLE development and disease pathogenesis.http://dx.doi.org/10.1080/08916934.2022.2128781cslemir-125bmir-9inflammationgene expression |
| spellingShingle | Denise de Queiroga Nascimento Isaura Isabelle Fonseca Gomes da Silva Camilla Albertina Dantas Lima André de Souza Cavalcanti Luciana Rodrigues Roberti Rosane Gomes de Paula Queiroz Virginia Paes Leme Ferriani Sergio Crovella Luciana Martins de Carvalho Paula Sandrin-Garcia Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE) Autoimmunity csle mir-125b mir-9 inflammation gene expression |
| title | Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE) |
| title_full | Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE) |
| title_fullStr | Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE) |
| title_full_unstemmed | Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE) |
| title_short | Expression of the miR-9-5p, miR-125b-5p and its target gene NFKB1 and TRAF6 in childhood-onset systemic lupus erythematosus (cSLE) |
| title_sort | expression of the mir 9 5p mir 125b 5p and its target gene nfkb1 and traf6 in childhood onset systemic lupus erythematosus csle |
| topic | csle mir-125b mir-9 inflammation gene expression |
| url | http://dx.doi.org/10.1080/08916934.2022.2128781 |
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