Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows
Knowledge of how vaginal and uterine microbiomes are composed is essential to prevent uterine diseases and to understand their pathogenesis. For this purpose, 50 dairy cows were involved in a prospective trial. Cows were clinically examined and vaginal and uterine swabs were taken (day 2, 4, 6 and 1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/3/405 |
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| author | Hanna Kronfeld Nicole Kemper Christina S. Hölzel |
| author_facet | Hanna Kronfeld Nicole Kemper Christina S. Hölzel |
| author_sort | Hanna Kronfeld |
| collection | DOAJ |
| description | Knowledge of how vaginal and uterine microbiomes are composed is essential to prevent uterine diseases and to understand their pathogenesis. For this purpose, 50 dairy cows were involved in a prospective trial. Cows were clinically examined and vaginal and uterine swabs were taken (day 2, 4, 6 and 14 p.p.), followed up by clinical examination on day 21 ± 1 and 41 ± 1. All samples (n = 364) were analyzed with cultural bacteriological methods and bacteria were identified by MALDI-TOF MS. In animals with a pathological puerperium, bacterial diversity was reduced and the genera <i>Fusobacteria</i> spp., <i>Bacteroides</i> spp. and <i>Helcococcus</i> spp. occurred significantly more frequently. By contrast, other well-known uterine pathogens such as <i>Escherichia</i> spp. and <i>Trueperella pyogenes</i> did not show significant differences between healthy and diseased cattle. Bacterial occurrences in the vagina and the uterus were sometimes correlated. Cows that suffered from puerperal disorders showed a significantly lower incidence of coagulase- negative staphylococci even before symptoms occurred. This may point towards a protective role of the vaginal microbiome. In addition, we discuss several shortcomings in calving hygiene that might have contributed to the high percentage of puerperal disorders on the study farm (58.7%). |
| first_indexed | 2024-03-09T20:13:51Z |
| format | Article |
| id | doaj.art-b1af0871646844aab6667ceb11e21573 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T20:13:51Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-b1af0871646844aab6667ceb11e215732023-11-24T00:05:51ZengMDPI AGAgriculture2077-04722022-03-0112340510.3390/agriculture12030405Vaginal and Uterine Microbiomes during Puerperium in Dairy CowsHanna Kronfeld0Nicole Kemper1Christina S. Hölzel2Department for Animal Hygiene and Animal Health, Institute of Animal Breeding and Husbandry, Christian-Albrechts-University, Olshausenstr. 40, 24098 Kiel, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, GermanyDepartment for Animal Hygiene and Animal Health, Institute of Animal Breeding and Husbandry, Christian-Albrechts-University, Olshausenstr. 40, 24098 Kiel, GermanyKnowledge of how vaginal and uterine microbiomes are composed is essential to prevent uterine diseases and to understand their pathogenesis. For this purpose, 50 dairy cows were involved in a prospective trial. Cows were clinically examined and vaginal and uterine swabs were taken (day 2, 4, 6 and 14 p.p.), followed up by clinical examination on day 21 ± 1 and 41 ± 1. All samples (n = 364) were analyzed with cultural bacteriological methods and bacteria were identified by MALDI-TOF MS. In animals with a pathological puerperium, bacterial diversity was reduced and the genera <i>Fusobacteria</i> spp., <i>Bacteroides</i> spp. and <i>Helcococcus</i> spp. occurred significantly more frequently. By contrast, other well-known uterine pathogens such as <i>Escherichia</i> spp. and <i>Trueperella pyogenes</i> did not show significant differences between healthy and diseased cattle. Bacterial occurrences in the vagina and the uterus were sometimes correlated. Cows that suffered from puerperal disorders showed a significantly lower incidence of coagulase- negative staphylococci even before symptoms occurred. This may point towards a protective role of the vaginal microbiome. In addition, we discuss several shortcomings in calving hygiene that might have contributed to the high percentage of puerperal disorders on the study farm (58.7%).https://www.mdpi.com/2077-0472/12/3/405microbiomemicrobial diversityuterine infectionmetritispuerperium |
| spellingShingle | Hanna Kronfeld Nicole Kemper Christina S. Hölzel Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows Agriculture microbiome microbial diversity uterine infection metritis puerperium |
| title | Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows |
| title_full | Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows |
| title_fullStr | Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows |
| title_full_unstemmed | Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows |
| title_short | Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows |
| title_sort | vaginal and uterine microbiomes during puerperium in dairy cows |
| topic | microbiome microbial diversity uterine infection metritis puerperium |
| url | https://www.mdpi.com/2077-0472/12/3/405 |
| work_keys_str_mv | AT hannakronfeld vaginalanduterinemicrobiomesduringpuerperiumindairycows AT nicolekemper vaginalanduterinemicrobiomesduringpuerperiumindairycows AT christinasholzel vaginalanduterinemicrobiomesduringpuerperiumindairycows |