Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
Abstract Pseudomonas is a ubiquitous genus that also causes human, animal and plant diseases. Most studies have focused on clinical P. aeruginosa strains from humans, but they are scarce on animal strains. This study was aimed to determine the occurrence of Pseudomonas spp. among faecal samples of h...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-68575-1 |
| _version_ | 1818347232648232960 |
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| author | Lidia Ruiz-Roldán Beatriz Rojo-Bezares María de Toro María López Paula Toledano Carmen Lozano Gabriela Chichón Lydia Alvarez-Erviti Carmen Torres Yolanda Sáenz |
| author_facet | Lidia Ruiz-Roldán Beatriz Rojo-Bezares María de Toro María López Paula Toledano Carmen Lozano Gabriela Chichón Lydia Alvarez-Erviti Carmen Torres Yolanda Sáenz |
| author_sort | Lidia Ruiz-Roldán |
| collection | DOAJ |
| description | Abstract Pseudomonas is a ubiquitous genus that also causes human, animal and plant diseases. Most studies have focused on clinical P. aeruginosa strains from humans, but they are scarce on animal strains. This study was aimed to determine the occurrence of Pseudomonas spp. among faecal samples of healthy animals, and to analyse their antimicrobial resistance, and pathogenicity. Among 704 animal faecal samples analysed, 133 Pseudomonas spp. isolates (23 species) were recovered from 46 samples (6.5%), and classified in 75 different PFGE patterns. Low antimicrobial resistance levels were found, being the highest to aztreonam (50.3%). Five sequence-types (ST1648, ST1711, ST2096, ST2194, ST2252), two serotypes (O:3, O:6), and three virulotypes (analysing 15 virulence and quorum-sensing genes) were observed among the 9 P. aeruginosa strains. Type-3-Secretion System genes were absent in the six O:3-serotype strains that additionally showed high cytotoxicity and produced higher biofilm biomass, phenazine pigments and motility than PAO1 control strain. In these six strains, the exlAB locus, and other virulence genotypes (e.g. RGP69 pathogenicity island) exclusive of PA7 outliers were detected by whole genome sequencing. This is the first description of the presence of the ExlA exolysin in P. aeruginosa from healthy animals, highlighting their pathological importance. |
| first_indexed | 2024-12-13T17:30:54Z |
| format | Article |
| id | doaj.art-b1afdd08946c44028408a43bf5705c3f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T17:30:54Z |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b1afdd08946c44028408a43bf5705c3f2022-12-21T23:37:03ZengNature PortfolioScientific Reports2045-23222020-07-0110111110.1038/s41598-020-68575-1Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detectionLidia Ruiz-Roldán0Beatriz Rojo-Bezares1María de Toro2María López3Paula Toledano4Carmen Lozano5Gabriela Chichón6Lydia Alvarez-Erviti7Carmen Torres8Yolanda Sáenz9Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Plataforma de Genómica y Bioinformática, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Neurobiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Área de Bioquímica y Biología Molecular, Complejo Científico-Tecnológico, Universidad de La RiojaÁrea de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR)Abstract Pseudomonas is a ubiquitous genus that also causes human, animal and plant diseases. Most studies have focused on clinical P. aeruginosa strains from humans, but they are scarce on animal strains. This study was aimed to determine the occurrence of Pseudomonas spp. among faecal samples of healthy animals, and to analyse their antimicrobial resistance, and pathogenicity. Among 704 animal faecal samples analysed, 133 Pseudomonas spp. isolates (23 species) were recovered from 46 samples (6.5%), and classified in 75 different PFGE patterns. Low antimicrobial resistance levels were found, being the highest to aztreonam (50.3%). Five sequence-types (ST1648, ST1711, ST2096, ST2194, ST2252), two serotypes (O:3, O:6), and three virulotypes (analysing 15 virulence and quorum-sensing genes) were observed among the 9 P. aeruginosa strains. Type-3-Secretion System genes were absent in the six O:3-serotype strains that additionally showed high cytotoxicity and produced higher biofilm biomass, phenazine pigments and motility than PAO1 control strain. In these six strains, the exlAB locus, and other virulence genotypes (e.g. RGP69 pathogenicity island) exclusive of PA7 outliers were detected by whole genome sequencing. This is the first description of the presence of the ExlA exolysin in P. aeruginosa from healthy animals, highlighting their pathological importance.https://doi.org/10.1038/s41598-020-68575-1 |
| spellingShingle | Lidia Ruiz-Roldán Beatriz Rojo-Bezares María de Toro María López Paula Toledano Carmen Lozano Gabriela Chichón Lydia Alvarez-Erviti Carmen Torres Yolanda Sáenz Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection Scientific Reports |
| title | Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection |
| title_full | Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection |
| title_fullStr | Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection |
| title_full_unstemmed | Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection |
| title_short | Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection |
| title_sort | antimicrobial resistance and virulence of pseudomonas spp among healthy animals concern about exolysin exla detection |
| url | https://doi.org/10.1038/s41598-020-68575-1 |
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