A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes
One of the most valuable traits in production and breeding is a sheep’s prolificacy which is influenced by several genes, one of which is the osteopontin ( OPN ) gene. Thus, this study aimed to determine the effect of genetic variation within the OPN gene on Awassi ewe prolificacy. Genomic DNA was e...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-05-01
|
| Series: | Bioinformatics and Biology Insights |
| Online Access: | https://doi.org/10.1177/11779322231172848 |
| _version_ | 1797827929286115328 |
|---|---|
| author | Tahreer M Al-Thuwaini Mohammed Baqur S Al-Shuhaib Ahmed F Kadhem Ahmed H Alkhammas |
| author_facet | Tahreer M Al-Thuwaini Mohammed Baqur S Al-Shuhaib Ahmed F Kadhem Ahmed H Alkhammas |
| author_sort | Tahreer M Al-Thuwaini |
| collection | DOAJ |
| description | One of the most valuable traits in production and breeding is a sheep’s prolificacy which is influenced by several genes, one of which is the osteopontin ( OPN ) gene. Thus, this study aimed to determine the effect of genetic variation within the OPN gene on Awassi ewe prolificacy. Genomic DNA was extracted from 123 single-progeny ewes and 109 twin ewes. Polymerase chain reaction (PCR) was used to amplify 4 sequence fragments (289, 275, 338, and 372 bp), representing exons 4, 5, 6, and 7 of the OPN gene. A 372 bp amplicon was identified with 3 different genotypes: TT, TC, and CC. Sequence analysis revealed a novel mutation in TC genotypes p.Q>R234. Statistical analysis revealed that the single nucleotide polymorphism (SNP) p.Q>R234 was associated with prolificacy. Ewes carrying the p.Q>R234 SNP had significantly ( P ⩽ .01) lower litter sizes, twinning rates, and lambing rates, and more days to lambing than those with the TC and TT genotypes. The p.Q>R234 SNP was confirmed to be responsible for lower litter size through logistic regression analysis. From these results, we can conclude that the missense variant p.Q>R234 adversely affects the traits of interest and shows that the p.Q>R234 SNP negatively influences the prolificacy of Awassi sheep. Based on this study, it is evident that ewes in this population carrying the p.Q>R234 SNP have a lower litter size and are less prolific. |
| first_indexed | 2024-04-09T12:56:17Z |
| format | Article |
| id | doaj.art-b1b11b39def74977b48271961fdcf892 |
| institution | Directory Open Access Journal |
| issn | 1177-9322 |
| language | English |
| last_indexed | 2024-04-09T12:56:17Z |
| publishDate | 2023-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Bioinformatics and Biology Insights |
| spelling | doaj.art-b1b11b39def74977b48271961fdcf8922023-05-13T12:33:29ZengSAGE PublishingBioinformatics and Biology Insights1177-93222023-05-011710.1177/11779322231172848A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi EwesTahreer M Al-ThuwainiMohammed Baqur S Al-ShuhaibAhmed F KadhemAhmed H AlkhammasOne of the most valuable traits in production and breeding is a sheep’s prolificacy which is influenced by several genes, one of which is the osteopontin ( OPN ) gene. Thus, this study aimed to determine the effect of genetic variation within the OPN gene on Awassi ewe prolificacy. Genomic DNA was extracted from 123 single-progeny ewes and 109 twin ewes. Polymerase chain reaction (PCR) was used to amplify 4 sequence fragments (289, 275, 338, and 372 bp), representing exons 4, 5, 6, and 7 of the OPN gene. A 372 bp amplicon was identified with 3 different genotypes: TT, TC, and CC. Sequence analysis revealed a novel mutation in TC genotypes p.Q>R234. Statistical analysis revealed that the single nucleotide polymorphism (SNP) p.Q>R234 was associated with prolificacy. Ewes carrying the p.Q>R234 SNP had significantly ( P ⩽ .01) lower litter sizes, twinning rates, and lambing rates, and more days to lambing than those with the TC and TT genotypes. The p.Q>R234 SNP was confirmed to be responsible for lower litter size through logistic regression analysis. From these results, we can conclude that the missense variant p.Q>R234 adversely affects the traits of interest and shows that the p.Q>R234 SNP negatively influences the prolificacy of Awassi sheep. Based on this study, it is evident that ewes in this population carrying the p.Q>R234 SNP have a lower litter size and are less prolific.https://doi.org/10.1177/11779322231172848 |
| spellingShingle | Tahreer M Al-Thuwaini Mohammed Baqur S Al-Shuhaib Ahmed F Kadhem Ahmed H Alkhammas A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes Bioinformatics and Biology Insights |
| title | A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes |
| title_full | A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes |
| title_fullStr | A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes |
| title_full_unstemmed | A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes |
| title_short | A Missense p.Q>R234 Mutation in the Osteopontin Gene Is Associated With the Prolificacy of Iraqi Awassi Ewes |
| title_sort | missense p q r234 mutation in the osteopontin gene is associated with the prolificacy of iraqi awassi ewes |
| url | https://doi.org/10.1177/11779322231172848 |
| work_keys_str_mv | AT tahreermalthuwaini amissensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT mohammedbaqursalshuhaib amissensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT ahmedfkadhem amissensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT ahmedhalkhammas amissensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT tahreermalthuwaini missensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT mohammedbaqursalshuhaib missensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT ahmedfkadhem missensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes AT ahmedhalkhammas missensepqr234mutationintheosteopontingeneisassociatedwiththeprolificacyofiraqiawassiewes |