Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy
In the context of cervical cancer prevention, where human papillomavirus (HPV) infection is pivotal, HPV testing is replacing Pap Smear in primary screening. This transition offers an opportunity for integrating self-sampling to enhance coverage. We evaluated the accuracy of HPV testing using self-c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1889 |
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| author | Marianna Martinelli Chiara Giubbi Maria Letizia Di Meo Federica Perdoni Rosario Musumeci Biagio Eugenio Leone Robert Fruscio Fabio Landoni Clementina Elvezia Cocuzza |
| author_facet | Marianna Martinelli Chiara Giubbi Maria Letizia Di Meo Federica Perdoni Rosario Musumeci Biagio Eugenio Leone Robert Fruscio Fabio Landoni Clementina Elvezia Cocuzza |
| author_sort | Marianna Martinelli |
| collection | DOAJ |
| description | In the context of cervical cancer prevention, where human papillomavirus (HPV) infection is pivotal, HPV testing is replacing Pap Smear in primary screening. This transition offers an opportunity for integrating self-sampling to enhance coverage. We evaluated the accuracy of HPV testing using self-collected urine and vaginal samples, comparing them to physician-collected cervical swabs. From a cohort of 245 women with abnormal cytology, we collected self-sampled vaginal, urine, and clinician-administered cervical specimens. Employing Anyplex™II HPV28 assay, outcomes revealed HPV positivity rates of 75.1% (cervical), 78.4% (vaginal), and 77.1% (urine). Significant, hr-HPV detection concordance was observed between self-taken cervical samples and clinical counterparts (k = 0.898 for vaginal; k = 0.715 for urine). This study extends beyond accuracy, highlighting self-collected sample efficacy in detecting high-grade cervical lesions. The insight underscores self-sampling’s role in bolstering participation and aligns with WHO’s goal to eliminate cervical cancer by 2030. |
| first_indexed | 2024-03-10T21:51:48Z |
| format | Article |
| id | doaj.art-b1b188fb3da946778367c0425f7f667b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:51:48Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b1b188fb3da946778367c0425f7f667b2023-11-19T13:23:09ZengMDPI AGViruses1999-49152023-09-01159188910.3390/v15091889Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to ColposcopyMarianna Martinelli0Chiara Giubbi1Maria Letizia Di Meo2Federica Perdoni3Rosario Musumeci4Biagio Eugenio Leone5Robert Fruscio6Fabio Landoni7Clementina Elvezia Cocuzza8Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyIRCCS San Gerardo dei Tintori, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyIn the context of cervical cancer prevention, where human papillomavirus (HPV) infection is pivotal, HPV testing is replacing Pap Smear in primary screening. This transition offers an opportunity for integrating self-sampling to enhance coverage. We evaluated the accuracy of HPV testing using self-collected urine and vaginal samples, comparing them to physician-collected cervical swabs. From a cohort of 245 women with abnormal cytology, we collected self-sampled vaginal, urine, and clinician-administered cervical specimens. Employing Anyplex™II HPV28 assay, outcomes revealed HPV positivity rates of 75.1% (cervical), 78.4% (vaginal), and 77.1% (urine). Significant, hr-HPV detection concordance was observed between self-taken cervical samples and clinical counterparts (k = 0.898 for vaginal; k = 0.715 for urine). This study extends beyond accuracy, highlighting self-collected sample efficacy in detecting high-grade cervical lesions. The insight underscores self-sampling’s role in bolstering participation and aligns with WHO’s goal to eliminate cervical cancer by 2030.https://www.mdpi.com/1999-4915/15/9/1889HPV infectionself-samplingHPV testingcervical cancerlow-risk HPV |
| spellingShingle | Marianna Martinelli Chiara Giubbi Maria Letizia Di Meo Federica Perdoni Rosario Musumeci Biagio Eugenio Leone Robert Fruscio Fabio Landoni Clementina Elvezia Cocuzza Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy Viruses HPV infection self-sampling HPV testing cervical cancer low-risk HPV |
| title | Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy |
| title_full | Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy |
| title_fullStr | Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy |
| title_full_unstemmed | Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy |
| title_short | Accuracy of Human Papillomavirus (HPV) Testing on Urine and Vaginal Self-Samples Compared to Clinician-Collected Cervical Sample in Women Referred to Colposcopy |
| title_sort | accuracy of human papillomavirus hpv testing on urine and vaginal self samples compared to clinician collected cervical sample in women referred to colposcopy |
| topic | HPV infection self-sampling HPV testing cervical cancer low-risk HPV |
| url | https://www.mdpi.com/1999-4915/15/9/1889 |
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