An Efficient Workflow for Screening and Stabilizing CRISPR/Cas9-Mediated Mutant Lines in <i>Bombyx mori</i>

An Efficient Workflow for Screening and Stabilizing CRISPR/Cas9-Mediated Mutant Lines in <i>Bombyx mori</i>

The domestic silkworm <i>Bombyx mori</i> is extensively studied as a model organism for lepidopteran genetics and has an economic value in silk production. Silkworms also have applications in biomedical and cosmetic industries, and the production of mutant <i>B. mori</i> stra...

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Bibliographic Details
Main Authors: Daniel Brady, Alessio Saviane, Silvia Cappellozza, Federica Sandrelli
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Methods and Protocols
Subjects:
CRISPR/Cas9
indel
molecular screening
heteroduplex assay
ARMS-PCR
<i>Bombyx mori</i>
Online Access:https://www.mdpi.com/2409-9279/4/1/4

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