Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells
The biosynthesis of silver nanoparticles using plant extracts is a safe and efficient method. Human periodontal stem cells (hPDLSCs) can promote periodontal tissue formation for regenerative therapy. The current study aimed to biosynthesize silver nanoparticles (AgNPs) using stem bark extracts from...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | OpenNano |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352952022000792 |
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| author | Jaruthai Prapaipittayakhun Supakorn Boonyuen Alvin Lim Teik Zheng Komsan Apinyauppatham Premjit Arpornmaeklong |
| author_facet | Jaruthai Prapaipittayakhun Supakorn Boonyuen Alvin Lim Teik Zheng Komsan Apinyauppatham Premjit Arpornmaeklong |
| author_sort | Jaruthai Prapaipittayakhun |
| collection | DOAJ |
| description | The biosynthesis of silver nanoparticles using plant extracts is a safe and efficient method. Human periodontal stem cells (hPDLSCs) can promote periodontal tissue formation for regenerative therapy. The current study aimed to biosynthesize silver nanoparticles (AgNPs) using stem bark extracts from Oroxylum indicum (L) Kurz (OI) as a reducing agent (OI/AgNPs) and investigate the biological properties of OI/AgNPs on human periodontal ligament stem cells (hPDLSCs). The OI/AgNPs were biosynthesized and characterized using UV–vis spectrophotometry (UV‒vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and zeta potential analyses. The hPDLSCs were stimulated with hydrogen peroxide (H2O2, H2O2-hPDLSCs) and bacterial lipopolysaccharide (LPS, LPS-hPDLSCs) and encapsulated in the hydrogel. After that, the levels of cell viability, interleukin-1beta (IL-1b), alkaline phosphatase (ALP) activity, and calcium content were measured and compared with quercetin (QT) as a reference flavonoid. The OI/AgNPs were stable spherical nanoparticles with a size range of 21.49 ± 0.32 nm, and biosynthesis enhanced the antioxidant and biological effects of the OI/AgNPs. The accumulation of OI/AgNPs in the cytoplasm of hPDLSCs with high levels of cell viability was demonstrated. OI/AgNPs increased the cell growth of H2O2-hPDLSCs and decreased the levels of IL-1b secretion from LPS-hPDLSCs. The OI/AgNPs increased the ALP activity and calcium content of the hPDLSCs. In conclusion, the biosynthesized OI/AgNPs were noncytotoxic and could protect hPDLSCs against oxidative stress and inflammatory stimuli and promote osteoblastic differentiation; thus, they are applicable for the regenerative treatment of peri‑implantitis. |
| first_indexed | 2024-04-11T00:55:45Z |
| format | Article |
| id | doaj.art-b1b809b25178430b9948244d7d7ed995 |
| institution | Directory Open Access Journal |
| issn | 2352-9520 |
| language | English |
| last_indexed | 2024-04-11T00:55:45Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | OpenNano |
| spelling | doaj.art-b1b809b25178430b9948244d7d7ed9952023-01-05T04:32:24ZengElsevierOpenNano2352-95202023-01-019100117Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cellsJaruthai Prapaipittayakhun0Supakorn Boonyuen1Alvin Lim Teik Zheng2Komsan Apinyauppatham3Premjit Arpornmaeklong4Master of Science Program in Dental Implantology, Faculty of Dentistry, Thammasat University-Rangsit campus, Khlong Luang, Pathum Thani 12121, ThailandDepartment of Chemistry, Faculty of Science and Technology, Thammasat University-Rangsit campus, Khlong Luang, Pathum Thani 12121, ThailandDepartment of Science and Technology, Faculty of Humanities, Management and Science, University Putra Malaysia Bintulu Campus, Jalan Nyabau, 97008 Bintulu, Sarawak, MalaysiaOral and Maxillofacial Surgery Division, Faculty of Dentistry, Thammasat University-Rangsit campus, Khlong Luang, Pathum Thani 12121, ThailandOral and Maxillofacial Surgery Division, Faculty of Dentistry, Thammasat University-Rangsit campus, Khlong Luang, Pathum Thani 12121, Thailand; Corresponding author: Assoc. Prof. Dr. Premjit Arpornmaeklong, Faculty of Dentistry, Thammasat University-Rangsit campus, Khlong Luang, Pathum Thani, 12121.The biosynthesis of silver nanoparticles using plant extracts is a safe and efficient method. Human periodontal stem cells (hPDLSCs) can promote periodontal tissue formation for regenerative therapy. The current study aimed to biosynthesize silver nanoparticles (AgNPs) using stem bark extracts from Oroxylum indicum (L) Kurz (OI) as a reducing agent (OI/AgNPs) and investigate the biological properties of OI/AgNPs on human periodontal ligament stem cells (hPDLSCs). The OI/AgNPs were biosynthesized and characterized using UV–vis spectrophotometry (UV‒vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and zeta potential analyses. The hPDLSCs were stimulated with hydrogen peroxide (H2O2, H2O2-hPDLSCs) and bacterial lipopolysaccharide (LPS, LPS-hPDLSCs) and encapsulated in the hydrogel. After that, the levels of cell viability, interleukin-1beta (IL-1b), alkaline phosphatase (ALP) activity, and calcium content were measured and compared with quercetin (QT) as a reference flavonoid. The OI/AgNPs were stable spherical nanoparticles with a size range of 21.49 ± 0.32 nm, and biosynthesis enhanced the antioxidant and biological effects of the OI/AgNPs. The accumulation of OI/AgNPs in the cytoplasm of hPDLSCs with high levels of cell viability was demonstrated. OI/AgNPs increased the cell growth of H2O2-hPDLSCs and decreased the levels of IL-1b secretion from LPS-hPDLSCs. The OI/AgNPs increased the ALP activity and calcium content of the hPDLSCs. In conclusion, the biosynthesized OI/AgNPs were noncytotoxic and could protect hPDLSCs against oxidative stress and inflammatory stimuli and promote osteoblastic differentiation; thus, they are applicable for the regenerative treatment of peri‑implantitis.http://www.sciencedirect.com/science/article/pii/S2352952022000792Anti-inflammatory agentCell differentiationCell encapsulationFlavonoidsMultifunctional nanoparticleSomatic adult stem cells |
| spellingShingle | Jaruthai Prapaipittayakhun Supakorn Boonyuen Alvin Lim Teik Zheng Komsan Apinyauppatham Premjit Arpornmaeklong Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells OpenNano Anti-inflammatory agent Cell differentiation Cell encapsulation Flavonoids Multifunctional nanoparticle Somatic adult stem cells |
| title | Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells |
| title_full | Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells |
| title_fullStr | Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells |
| title_full_unstemmed | Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells |
| title_short | Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on human periodontal ligament stem cells |
| title_sort | biologic effects of biosynthesized oroxylum indicum silver nanoparticles on human periodontal ligament stem cells |
| topic | Anti-inflammatory agent Cell differentiation Cell encapsulation Flavonoids Multifunctional nanoparticle Somatic adult stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2352952022000792 |
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