The state of the liver hydrogen sulfide system in rats with experimental nephropathy under conditions of melatonin introduction
Summary. Metabolic disorders occurring with nephro­pathy are associated with the production of free radicals resulting in structural-functional disturbances of the cellular membranes of different organs and systems including the liver. Hydrogen sulfide (H2S) is a gas transmitter and an impor...
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| Format: | Article |
| Language: | English |
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I. Horbachevsky Ternopil National Medical University
2021-02-01
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| Series: | Вісник медичних і біологічних досліджень |
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| Online Access: | https://bmbr.com.ua/journals/tom-7-1-2021/stan-sistemi-gidrogen-sulfidu-pechinki-shchuriv-pri-eksperimentalniy-nefropatiyi-za-umov-vvedennya-melatoninu |
| Summary: | Summary. Metabolic disorders occurring with nephro­pathy are associated with the production of free radicals resulting in structural-functional disturbances of the cellular membranes of different organs and systems including the liver. Hydrogen sulfide (H2S) is a gas transmitter and an important signal molecule regulating a number of physiological and pathophysiological processes. Melatonin as a hormone produced by the pineal body possesses immune stimulating, disintoxicating and antioxidant properties. Meanwhile, all the mechanisms of melatonin action still remain uncertain, in particular its effect on the hydrogen sulfide system with nephropathy. The aim of the study – to determine melatonin effect on the hydrogen sulfide system state in the liver of rats under conditions of experimental nephropathy. Materials and Methods. The experiment was carried out on 84 nonlinear male rats with the body weight of 0.16–0.18 kg. Experimental nephropathy was simulated by means of a single introduction of folic acid into the peritoneum in the dose of 250 mg/kg of the body weight. Melatonin was introduced into the stomach during 7 days in the dose of 10 mg/kg after nephropathy was simulated. The production and concentration of hydrogen sulfide, activity of H2S-generating enzymes such as cystathionine γ-lyase (CSE), cystathionine β- synthase (СBS) and cysteine aminotransferase (САТ) were determined in the post-mitochondrial liver fraction of rats. Results. With experimental nephropathy the production and concentration of H2S 27.9 % and 45.2 % decreased respectively, and the activity of H2S-generating enzymes decreased as well: CSE by 31.7 %, СBS by 32.1 % and САТ by 32.7 %, in comparison with the group of control animals. Melatonin introduction promoted increase of H2S-production and concentration by 20.9 % and 33.0 %, as well as the activity of H2S-producing enzymes СBS and САТ by 23.7 % and 25.4 % respectively, in comparison with the parameters of animals with nephropathy, but they differed reliably from the parameters of the control animals. Conclusions. Nephropathy leads to decrease of the concentration and production of Н2S in the liver of animals due to inhibition of H2S-producing activity of enzymes gene­rating Н2S. Melatonin introduction promoted increase of hydrogen sulfide content both at the expense of increased activity of СВS and САТ, and probably at the expense of a direct participation of melatonin in neutralization of free radicals and reduction of protein oxidative modification, and the examined enzymes in particular |
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| ISSN: | 2706-6282 2706-6290 |