Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells
Background: The beneficial effects of compound K (CK) on different chronic diseases have been shown to be at least related to antioxidant action. Nevertheless, since its antioxidant activity in human retinal pigment epithelial (RPE) cells is still unknown, here we investigated whether CK alleviates...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845322001233 |
| _version_ | 1811162756696506368 |
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| author | Cheol Park Hee-Jae Cha Kyoung-Seob Song Heui-Soo Kim EunJin Bang Hyesook Lee Cheng-Yun Jin Gi-Young Kim Yung Hyun Choi |
| author_facet | Cheol Park Hee-Jae Cha Kyoung-Seob Song Heui-Soo Kim EunJin Bang Hyesook Lee Cheng-Yun Jin Gi-Young Kim Yung Hyun Choi |
| author_sort | Cheol Park |
| collection | DOAJ |
| description | Background: The beneficial effects of compound K (CK) on different chronic diseases have been shown to be at least related to antioxidant action. Nevertheless, since its antioxidant activity in human retinal pigment epithelial (RPE) cells is still unknown, here we investigated whether CK alleviates oxidative stress-stimulated damage in RPE ARPE-19 cells. Methods: The cytoprotective consequence of CK in hydrogen peroxide (H2O2)-treated cells was evaluated by cell viability, DNA damage, and apoptosis assays. Fluorescence analysis and immunoblotting were performed to investigate the inhibitory action of CK on reactive oxygen species (ROS) production and mitochondrial dysfunction. Results: H2O2-promoted cytotoxicity, oxidative stress, DNA damage, mitochondrial impairment, and apoptosis were significantly attenuated by CK in ARPE-19 cells. Furthermore, nuclear factor erythroid 2-related factor 2 (Nrf2) phosphorylation level and its shuttling to the nucleus were increased, which was correlated with upregulated activation of heme oxygenase-1 (HO-1). However, zinc protoporphyrin, a blocker of HO-1, significantly abrogated the preventive action of CK in H2O2-treated ARPE-19 cells. Conclusion: This study indicates that activation of Nrf2/HO-1 signaling by CK plays an important role in rescuing ARPE-19 cells from oxidative cellular damage. |
| first_indexed | 2024-04-10T06:34:20Z |
| format | Article |
| id | doaj.art-b1bcbe2766f5403183aa6aca9e5a0e6a |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-10T06:34:20Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-b1bcbe2766f5403183aa6aca9e5a0e6a2023-03-01T04:31:13ZengElsevierJournal of Ginseng Research1226-84532023-03-01472311318Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cellsCheol Park0Hee-Jae Cha1Kyoung-Seob Song2Heui-Soo Kim3EunJin Bang4Hyesook Lee5Cheng-Yun Jin6Gi-Young Kim7Yung Hyun Choi8Division of Basic Sciences, Dong-eui University, Busan, Republic of KoreaCollege of Medicine, Kosin University, Busan, Republic of KoreaCollege of Medicine, Kosin University, Busan, Republic of KoreaDepartment of Biological Sciences, Pusan National University, Busan, Republic of KoreaAnti-Aging Research Center and Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan, Republic of KoreaDepartment of Convergence Medicine, Pusan National University, Yangsan, Republic of KoreaCollege of Pharmacy, Zhengzhou University, Zhengzhou, ChinaDepartment of Marine Life Science, Jeju National University, Jeju, Republic of KoreaAnti-Aging Research Center and Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan, Republic of Korea; Corresponding author. Department of Biochemistry, Dong-eui University College of Korean Medicine, 52-57, Yangjeong-ro, Busanjin-gu Busan, 47227, Republic of Korea.Background: The beneficial effects of compound K (CK) on different chronic diseases have been shown to be at least related to antioxidant action. Nevertheless, since its antioxidant activity in human retinal pigment epithelial (RPE) cells is still unknown, here we investigated whether CK alleviates oxidative stress-stimulated damage in RPE ARPE-19 cells. Methods: The cytoprotective consequence of CK in hydrogen peroxide (H2O2)-treated cells was evaluated by cell viability, DNA damage, and apoptosis assays. Fluorescence analysis and immunoblotting were performed to investigate the inhibitory action of CK on reactive oxygen species (ROS) production and mitochondrial dysfunction. Results: H2O2-promoted cytotoxicity, oxidative stress, DNA damage, mitochondrial impairment, and apoptosis were significantly attenuated by CK in ARPE-19 cells. Furthermore, nuclear factor erythroid 2-related factor 2 (Nrf2) phosphorylation level and its shuttling to the nucleus were increased, which was correlated with upregulated activation of heme oxygenase-1 (HO-1). However, zinc protoporphyrin, a blocker of HO-1, significantly abrogated the preventive action of CK in H2O2-treated ARPE-19 cells. Conclusion: This study indicates that activation of Nrf2/HO-1 signaling by CK plays an important role in rescuing ARPE-19 cells from oxidative cellular damage.http://www.sciencedirect.com/science/article/pii/S1226845322001233ApoptosisARPE-19 cellsCompound KNrf2/HO-1Oxidative stress |
| spellingShingle | Cheol Park Hee-Jae Cha Kyoung-Seob Song Heui-Soo Kim EunJin Bang Hyesook Lee Cheng-Yun Jin Gi-Young Kim Yung Hyun Choi Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells Journal of Ginseng Research Apoptosis ARPE-19 cells Compound K Nrf2/HO-1 Oxidative stress |
| title | Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells |
| title_full | Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells |
| title_fullStr | Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells |
| title_full_unstemmed | Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells |
| title_short | Nrf2-mediated activation of HO-1 is required in the blocking effect of compound K, a ginseng saponin metabolite, against oxidative stress damage in ARPE-19 human retinal pigment epithelial cells |
| title_sort | nrf2 mediated activation of ho 1 is required in the blocking effect of compound k a ginseng saponin metabolite against oxidative stress damage in arpe 19 human retinal pigment epithelial cells |
| topic | Apoptosis ARPE-19 cells Compound K Nrf2/HO-1 Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1226845322001233 |
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