Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil
There are large areas of contaminated soils with low- and medium-concentration of polycyclic aromatic hydrocarbons (PAHs) in the coldest regions of the earth, potentially threatening ecological safety and human health. Using maize cobs (MC) as the carrier, two cold-adaptive PAHs-degrading microorgan...
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Elsevier
2021-07-01
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| Series: | Environmental Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266676572100034X |
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| author | Dan Su Yu Pu ChunJuan Gong Zhenli He |
| author_facet | Dan Su Yu Pu ChunJuan Gong Zhenli He |
| author_sort | Dan Su |
| collection | DOAJ |
| description | There are large areas of contaminated soils with low- and medium-concentration of polycyclic aromatic hydrocarbons (PAHs) in the coldest regions of the earth, potentially threatening ecological safety and human health. Using maize cobs (MC) as the carrier, two cold-adaptive PAHs-degrading microorganisms, bacterial (Pseudomonas sp., SDR4) and fungal (Mortierella alpine, JDR7) strains were co-immobilized. The degradation characteristics of phenanthrene (Phe), pyrene (Pyr) and benzo[a]pyrene (BaP) in freeze-thawed soil by both co-immobilized and free microorganism were studied. The removal rate of Phe, Pyr and BaP using the co-immobilized fungal-bacterial consortium within 60 d was 59.2±3.7%, 46.6±3.3%, and 36.8±2.7%, respectively, which was obviously higher than that of free fungal-bacterial consortium in the same time. Ten percent (w/w) was the optimal amount of inoculum for PAH degradation in the co-immobilized fungal-bacterial consortium. Under low-temperature conditions, when the initial concentration of PAH was between 10-100 mg•kg−1, the immobilized cold-adaptive fungal-bacterial consortium displayed the desired degradation of PAHs. The scanning electronic microscope (SEM) observation and mass transmission showed that the microstructure of co-immobilized mixed system was beneficial to the growth of SDR4 and JDR7 at low temperature. These results imply that the cold-adaptive fungal-bacterial consortium, co-immobilized on MC, has the potential for application in remediating PAH contaminated soil under the freeze-thawing environment. |
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| last_indexed | 2024-12-17T07:11:16Z |
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| spelling | doaj.art-b1bd5c1d0931464daedb33ad4445d2c12022-12-21T21:59:02ZengElsevierEnvironmental Advances2666-76572021-07-014100063Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soilDan Su0Yu Pu1ChunJuan Gong2Zhenli He3College of Environment Science, Liaoning University, 66 Chongshan Middle Street, Huanggu District, Shenyang, 110036, PR China.; Corresponding author.College of Environment Science, Liaoning University, 66 Chongshan Middle Street, Huanggu District, Shenyang, 110036, PR China.Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR ChinaUniversity of Florida-IFAS, Indian River Research and Education Center, Fort Pierce, FL 34945-3138, USAThere are large areas of contaminated soils with low- and medium-concentration of polycyclic aromatic hydrocarbons (PAHs) in the coldest regions of the earth, potentially threatening ecological safety and human health. Using maize cobs (MC) as the carrier, two cold-adaptive PAHs-degrading microorganisms, bacterial (Pseudomonas sp., SDR4) and fungal (Mortierella alpine, JDR7) strains were co-immobilized. The degradation characteristics of phenanthrene (Phe), pyrene (Pyr) and benzo[a]pyrene (BaP) in freeze-thawed soil by both co-immobilized and free microorganism were studied. The removal rate of Phe, Pyr and BaP using the co-immobilized fungal-bacterial consortium within 60 d was 59.2±3.7%, 46.6±3.3%, and 36.8±2.7%, respectively, which was obviously higher than that of free fungal-bacterial consortium in the same time. Ten percent (w/w) was the optimal amount of inoculum for PAH degradation in the co-immobilized fungal-bacterial consortium. Under low-temperature conditions, when the initial concentration of PAH was between 10-100 mg•kg−1, the immobilized cold-adaptive fungal-bacterial consortium displayed the desired degradation of PAHs. The scanning electronic microscope (SEM) observation and mass transmission showed that the microstructure of co-immobilized mixed system was beneficial to the growth of SDR4 and JDR7 at low temperature. These results imply that the cold-adaptive fungal-bacterial consortium, co-immobilized on MC, has the potential for application in remediating PAH contaminated soil under the freeze-thawing environment.http://www.sciencedirect.com/science/article/pii/S266676572100034XImmobilized microorganismsCold regionsOrganic contaminantsRemediationSoil pollutionAbbreviations, PAHs, Polycyclic aromatic hydrocarbons |
| spellingShingle | Dan Su Yu Pu ChunJuan Gong Zhenli He Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil Environmental Advances Immobilized microorganisms Cold regions Organic contaminants Remediation Soil pollution Abbreviations, PAHs, Polycyclic aromatic hydrocarbons |
| title | Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil |
| title_full | Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil |
| title_fullStr | Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil |
| title_full_unstemmed | Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil |
| title_short | Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil |
| title_sort | application of cold adaptive pseudomonas sp sdr4 and mortierella alpina jdr7 co immobilized on maize cob in remediating pah contaminated freeze thawed soil |
| topic | Immobilized microorganisms Cold regions Organic contaminants Remediation Soil pollution Abbreviations, PAHs, Polycyclic aromatic hydrocarbons |
| url | http://www.sciencedirect.com/science/article/pii/S266676572100034X |
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