Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature
Evidence from our lab and others indicates the vascular effects of dietary blueberries. In the present study, we determined dietary blueberries’ dose- and time-dependent effects on diabetic vasculature and their association with gut microbes. Seven-week-old <i>db</i>/<i>db</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/8/1527 |
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| author | Adhini Kuppuswamy Satheesh Babu Chrissa Petersen Henry A. Paz Kai Benedict Miley Nguyen Madison Putich Miguel Saldivar-Gonzalez Ying Zhong Sydney Larsen Umesh D. Wankhade Pon Velayutham Anandh Babu |
| author_facet | Adhini Kuppuswamy Satheesh Babu Chrissa Petersen Henry A. Paz Kai Benedict Miley Nguyen Madison Putich Miguel Saldivar-Gonzalez Ying Zhong Sydney Larsen Umesh D. Wankhade Pon Velayutham Anandh Babu |
| author_sort | Adhini Kuppuswamy Satheesh Babu |
| collection | DOAJ |
| description | Evidence from our lab and others indicates the vascular effects of dietary blueberries. In the present study, we determined dietary blueberries’ dose- and time-dependent effects on diabetic vasculature and their association with gut microbes. Seven-week-old <i>db</i>/<i>db</i> diabetic male mice were fed a diet supplemented with ± freeze-dried wild blueberry powder (FD-BB) for 4, 8, or 12 weeks (three cohorts). Diets contained 0%, 1.23%, 2.46%, and 3.7% of FD-BB, equivalent to 0, ½, 1, and 1.5 human servings of wild blueberries, respectively. The non-diabetic <i>db</i>/<i>+</i> mice fed a standard diet served as controls. Metabolic parameters, vascular inflammation, and gut microbiome were assessed. Dietary supplementation of 3.7% FD-BB improved vascular inflammation in diabetic mice without improving systemic milieu in all three cohorts. Blueberries improved diabetes-induced gut dysbiosis depending on blueberry dosage and treatment duration. Spearman’s correlation indicated that the opportunistic microbes and commensal microbes were positively and negatively associated with indices of vascular inflammation, respectively. Dietary blueberries reduced the opportunistic microbe that was positively associated with vascular inflammation (<i>Desulfovibrio</i>), and increased the commensal microbe that was negatively associated with vascular inflammation (<i>Akkermansia</i>). Dietary blueberries could be a potential adjunct strategy to beneficially modulate gut microbes and improve vascular complications in diabetes. |
| first_indexed | 2024-03-11T00:10:50Z |
| format | Article |
| id | doaj.art-b1c263c74e294c13a01f91c77f0bde14 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T00:10:50Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-b1c263c74e294c13a01f91c77f0bde142023-11-19T00:01:13ZengMDPI AGAntioxidants2076-39212023-07-01128152710.3390/antiox12081527Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial SignatureAdhini Kuppuswamy Satheesh Babu0Chrissa Petersen1Henry A. Paz2Kai Benedict3Miley Nguyen4Madison Putich5Miguel Saldivar-Gonzalez6Ying Zhong7Sydney Larsen8Umesh D. Wankhade9Pon Velayutham Anandh Babu10Department of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USAArkansas Children’s Nutrition Center, Little Rock, Arkansas, AR 72205, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USAArkansas Children’s Nutrition Center, Little Rock, Arkansas, AR 72205, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USAArkansas Children’s Nutrition Center, Little Rock, Arkansas, AR 72205, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USAEvidence from our lab and others indicates the vascular effects of dietary blueberries. In the present study, we determined dietary blueberries’ dose- and time-dependent effects on diabetic vasculature and their association with gut microbes. Seven-week-old <i>db</i>/<i>db</i> diabetic male mice were fed a diet supplemented with ± freeze-dried wild blueberry powder (FD-BB) for 4, 8, or 12 weeks (three cohorts). Diets contained 0%, 1.23%, 2.46%, and 3.7% of FD-BB, equivalent to 0, ½, 1, and 1.5 human servings of wild blueberries, respectively. The non-diabetic <i>db</i>/<i>+</i> mice fed a standard diet served as controls. Metabolic parameters, vascular inflammation, and gut microbiome were assessed. Dietary supplementation of 3.7% FD-BB improved vascular inflammation in diabetic mice without improving systemic milieu in all three cohorts. Blueberries improved diabetes-induced gut dysbiosis depending on blueberry dosage and treatment duration. Spearman’s correlation indicated that the opportunistic microbes and commensal microbes were positively and negatively associated with indices of vascular inflammation, respectively. Dietary blueberries reduced the opportunistic microbe that was positively associated with vascular inflammation (<i>Desulfovibrio</i>), and increased the commensal microbe that was negatively associated with vascular inflammation (<i>Akkermansia</i>). Dietary blueberries could be a potential adjunct strategy to beneficially modulate gut microbes and improve vascular complications in diabetes.https://www.mdpi.com/2076-3921/12/8/1527blueberriesdose/time-dependent effectvascular inflammationcardiovasculargut microbescorrelation |
| spellingShingle | Adhini Kuppuswamy Satheesh Babu Chrissa Petersen Henry A. Paz Kai Benedict Miley Nguyen Madison Putich Miguel Saldivar-Gonzalez Ying Zhong Sydney Larsen Umesh D. Wankhade Pon Velayutham Anandh Babu Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature Antioxidants blueberries dose/time-dependent effect vascular inflammation cardiovascular gut microbes correlation |
| title | Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature |
| title_full | Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature |
| title_fullStr | Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature |
| title_full_unstemmed | Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature |
| title_short | Dose- and Time-Dependent Effect of Dietary Blueberries on Diabetic Vasculature Is Correlated with Gut Microbial Signature |
| title_sort | dose and time dependent effect of dietary blueberries on diabetic vasculature is correlated with gut microbial signature |
| topic | blueberries dose/time-dependent effect vascular inflammation cardiovascular gut microbes correlation |
| url | https://www.mdpi.com/2076-3921/12/8/1527 |
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