A polycystin-type transient receptor potential (Trp) channel that is activated by ATP
ATP and ADP are ancient extra-cellular signalling molecules that in Dictyostelium amoebae cause rapid, transient increases in cytosolic calcium due to an influx through the plasma membrane. This response is independent of hetero-trimeric G-proteins, the putative IP3 receptor IplA and all P2X channel...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2017-02-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/6/2/200 |
| _version_ | 1818436226878799872 |
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| author | David Traynor Robert R. Kay |
| author_facet | David Traynor Robert R. Kay |
| author_sort | David Traynor |
| collection | DOAJ |
| description | ATP and ADP are ancient extra-cellular signalling molecules that in Dictyostelium amoebae cause rapid, transient increases in cytosolic calcium due to an influx through the plasma membrane. This response is independent of hetero-trimeric G-proteins, the putative IP3 receptor IplA and all P2X channels. We show, unexpectedly, that it is abolished in mutants of the polycystin-type transient receptor potential channel, TrpP. Responses to the chemoattractants cyclic-AMP and folic acid are unaffected in TrpP mutants. We report that the DIF morphogens, cyclic-di-GMP, GABA, glutamate and adenosine all induce strong cytoplasmic calcium responses, likewise independently of TrpP. Thus, TrpP is dedicated to purinergic signalling. ATP treatment causes cell blebbing within seconds but this does not require TrpP, implicating a separate purinergic receptor. We could detect no effect of ATP on chemotaxis and TrpP mutants grow, chemotax and develop almost normally in standard conditions. No gating ligand is known for the human homologue of TrpP, polycystin-2, which causes polycystic kidney disease. Our results now show that TrpP mediates purinergic signalling in Dictyostelium and is directly or indirectly gated by ATP. |
| first_indexed | 2024-12-14T17:05:25Z |
| format | Article |
| id | doaj.art-b1c3ff35ace947db940fd10447ee968d |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-14T17:05:25Z |
| publishDate | 2017-02-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-b1c3ff35ace947db940fd10447ee968d2022-12-21T22:53:44ZengThe Company of BiologistsBiology Open2046-63902017-02-016220020910.1242/bio.020685020685A polycystin-type transient receptor potential (Trp) channel that is activated by ATPDavid Traynor0Robert R. Kay1 MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB1 0QH, UK MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB1 0QH, UK ATP and ADP are ancient extra-cellular signalling molecules that in Dictyostelium amoebae cause rapid, transient increases in cytosolic calcium due to an influx through the plasma membrane. This response is independent of hetero-trimeric G-proteins, the putative IP3 receptor IplA and all P2X channels. We show, unexpectedly, that it is abolished in mutants of the polycystin-type transient receptor potential channel, TrpP. Responses to the chemoattractants cyclic-AMP and folic acid are unaffected in TrpP mutants. We report that the DIF morphogens, cyclic-di-GMP, GABA, glutamate and adenosine all induce strong cytoplasmic calcium responses, likewise independently of TrpP. Thus, TrpP is dedicated to purinergic signalling. ATP treatment causes cell blebbing within seconds but this does not require TrpP, implicating a separate purinergic receptor. We could detect no effect of ATP on chemotaxis and TrpP mutants grow, chemotax and develop almost normally in standard conditions. No gating ligand is known for the human homologue of TrpP, polycystin-2, which causes polycystic kidney disease. Our results now show that TrpP mediates purinergic signalling in Dictyostelium and is directly or indirectly gated by ATP.http://bio.biologists.org/content/6/2/200Trp channelPolycystin-2Purinergic signallingATPDIFDictyostelium |
| spellingShingle | David Traynor Robert R. Kay A polycystin-type transient receptor potential (Trp) channel that is activated by ATP Biology Open Trp channel Polycystin-2 Purinergic signalling ATP DIF Dictyostelium |
| title | A polycystin-type transient receptor potential (Trp) channel that is activated by ATP |
| title_full | A polycystin-type transient receptor potential (Trp) channel that is activated by ATP |
| title_fullStr | A polycystin-type transient receptor potential (Trp) channel that is activated by ATP |
| title_full_unstemmed | A polycystin-type transient receptor potential (Trp) channel that is activated by ATP |
| title_short | A polycystin-type transient receptor potential (Trp) channel that is activated by ATP |
| title_sort | polycystin type transient receptor potential trp channel that is activated by atp |
| topic | Trp channel Polycystin-2 Purinergic signalling ATP DIF Dictyostelium |
| url | http://bio.biologists.org/content/6/2/200 |
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