Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility
PR domain containing 9 (Prdm9) is specifying hotspots of meiotic recombination but in hybrids between two mouse subspecies Prdm9 controls failure of meiotic chromosome synapsis and hybrid male sterility. We have previously reported that Prdm9-controlled asynapsis and meiotic arrest are conditioned b...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-12-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/42511 |
| _version_ | 1811181045777694720 |
|---|---|
| author | Liu Wang Barbora Valiskova Jiri Forejt |
| author_facet | Liu Wang Barbora Valiskova Jiri Forejt |
| author_sort | Liu Wang |
| collection | DOAJ |
| description | PR domain containing 9 (Prdm9) is specifying hotspots of meiotic recombination but in hybrids between two mouse subspecies Prdm9 controls failure of meiotic chromosome synapsis and hybrid male sterility. We have previously reported that Prdm9-controlled asynapsis and meiotic arrest are conditioned by the inter-subspecific heterozygosity of the hybrid genome and we presumed that the insufficient number of properly repaired PRDM9-dependent DNA double-strand breaks (DSBs) causes asynapsis of chromosomes and meiotic arrest (Gregorova et al., 2018). We now extend the evidence for the lack of properly processed DSBs by improving meiotic chromosome synapsis with exogenous DSBs. A single injection of chemotherapeutic drug cisplatin increased frequency of RPA and DMC1 foci at the zygotene stage of sterile hybrids, enhanced homolog recognition and increased the proportion of spermatocytes with fully synapsed homologs at pachytene. The results bring a new evidence for a DSB-dependent mechanism of synapsis failure and infertility of intersubspecific hybrids. |
| first_indexed | 2024-04-11T09:13:00Z |
| format | Article |
| id | doaj.art-b1c6bf96fb4747848e32560c73f5dc99 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:13:00Z |
| publishDate | 2018-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b1c6bf96fb4747848e32560c73f5dc992022-12-22T04:32:28ZengeLife Sciences Publications LtdeLife2050-084X2018-12-01710.7554/eLife.42511Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterilityLiu Wang0Barbora Valiskova1Jiri Forejt2https://orcid.org/0000-0002-2793-3623BIOCEV Division, Institute of Molecular Genetics, Czech Academy of Sciences, Vestec, Czech RepublicBIOCEV Division, Institute of Molecular Genetics, Czech Academy of Sciences, Vestec, Czech Republic; Faculty of Science, Charles University, Prague, Czech RepublicBIOCEV Division, Institute of Molecular Genetics, Czech Academy of Sciences, Vestec, Czech RepublicPR domain containing 9 (Prdm9) is specifying hotspots of meiotic recombination but in hybrids between two mouse subspecies Prdm9 controls failure of meiotic chromosome synapsis and hybrid male sterility. We have previously reported that Prdm9-controlled asynapsis and meiotic arrest are conditioned by the inter-subspecific heterozygosity of the hybrid genome and we presumed that the insufficient number of properly repaired PRDM9-dependent DNA double-strand breaks (DSBs) causes asynapsis of chromosomes and meiotic arrest (Gregorova et al., 2018). We now extend the evidence for the lack of properly processed DSBs by improving meiotic chromosome synapsis with exogenous DSBs. A single injection of chemotherapeutic drug cisplatin increased frequency of RPA and DMC1 foci at the zygotene stage of sterile hybrids, enhanced homolog recognition and increased the proportion of spermatocytes with fully synapsed homologs at pachytene. The results bring a new evidence for a DSB-dependent mechanism of synapsis failure and infertility of intersubspecific hybrids.https://elifesciences.org/articles/42511meiosishybrid sterilityPrdm9DNA double-strand breaks |
| spellingShingle | Liu Wang Barbora Valiskova Jiri Forejt Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility eLife meiosis hybrid sterility Prdm9 DNA double-strand breaks |
| title | Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility |
| title_full | Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility |
| title_fullStr | Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility |
| title_full_unstemmed | Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility |
| title_short | Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility |
| title_sort | cisplatin induced dna double strand breaks promote meiotic chromosome synapsis in prdm9 controlled mouse hybrid sterility |
| topic | meiosis hybrid sterility Prdm9 DNA double-strand breaks |
| url | https://elifesciences.org/articles/42511 |
| work_keys_str_mv | AT liuwang cisplatininduceddnadoublestrandbreakspromotemeioticchromosomesynapsisinprdm9controlledmousehybridsterility AT barboravaliskova cisplatininduceddnadoublestrandbreakspromotemeioticchromosomesynapsisinprdm9controlledmousehybridsterility AT jiriforejt cisplatininduceddnadoublestrandbreakspromotemeioticchromosomesynapsisinprdm9controlledmousehybridsterility |