Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat
Growth Arrest-Specific 1 (Gas1) is a pleiotropic protein with different functions, in the adult kidney Gas1 acts as an endogenous inhibitor of cell proliferation but it is also necessary for the maintenance and proliferation of Renal Progenitor Cells (RPC) during early development, thus it fulfills...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124827/?tool=EBI |
| _version_ | 1797839134595743744 |
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| author | Andrea Cetina-Palma Carmen Namorado-Tónix Rafael Rodríguez-Muñoz Paula Vergara José Luis Reyes-Sánchez José Segovia |
| author_facet | Andrea Cetina-Palma Carmen Namorado-Tónix Rafael Rodríguez-Muñoz Paula Vergara José Luis Reyes-Sánchez José Segovia |
| author_sort | Andrea Cetina-Palma |
| collection | DOAJ |
| description | Growth Arrest-Specific 1 (Gas1) is a pleiotropic protein with different functions, in the adult kidney Gas1 acts as an endogenous inhibitor of cell proliferation but it is also necessary for the maintenance and proliferation of Renal Progenitor Cells (RPC) during early development, thus it fulfills important functions in the adult kidney. However, it is not known whether or not Gas1 is expressed during postnatal development, a critical stage for renal maturation. For this reason, the main objective of this work was to characterize the expression pattern of Gas1 in the different regions of the kidney by immunofluorescence and Western blot analysis during the postnatal development of the rat. We found that Gas1 is present and has a differential expression pattern in the various regions of the nephron during postnatal development. We observed that the highest levels of expression of Gas1 occur in the adult, however, Gas1 is also expressed in RPC and interestingly, the expression of RPC markers such as the Neural cell adhesion molecule (NCAM) and Cluster of differentiation 24 (CD24) were found to have an inverse pattern of expression to Gas1 (decreases as the kidney matures) during postnatal renal maturation, this indicates a role for Gas1 in the regulation of renal cell proliferation at this stage of development. |
| first_indexed | 2024-04-09T15:52:16Z |
| format | Article |
| id | doaj.art-b1c74974ed05444da48e5ba3a46f8f2e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T15:52:16Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b1c74974ed05444da48e5ba3a46f8f2e2023-04-26T05:32:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01184Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the ratAndrea Cetina-PalmaCarmen Namorado-TónixRafael Rodríguez-MuñozPaula VergaraJosé Luis Reyes-SánchezJosé SegoviaGrowth Arrest-Specific 1 (Gas1) is a pleiotropic protein with different functions, in the adult kidney Gas1 acts as an endogenous inhibitor of cell proliferation but it is also necessary for the maintenance and proliferation of Renal Progenitor Cells (RPC) during early development, thus it fulfills important functions in the adult kidney. However, it is not known whether or not Gas1 is expressed during postnatal development, a critical stage for renal maturation. For this reason, the main objective of this work was to characterize the expression pattern of Gas1 in the different regions of the kidney by immunofluorescence and Western blot analysis during the postnatal development of the rat. We found that Gas1 is present and has a differential expression pattern in the various regions of the nephron during postnatal development. We observed that the highest levels of expression of Gas1 occur in the adult, however, Gas1 is also expressed in RPC and interestingly, the expression of RPC markers such as the Neural cell adhesion molecule (NCAM) and Cluster of differentiation 24 (CD24) were found to have an inverse pattern of expression to Gas1 (decreases as the kidney matures) during postnatal renal maturation, this indicates a role for Gas1 in the regulation of renal cell proliferation at this stage of development.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124827/?tool=EBI |
| spellingShingle | Andrea Cetina-Palma Carmen Namorado-Tónix Rafael Rodríguez-Muñoz Paula Vergara José Luis Reyes-Sánchez José Segovia Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat PLoS ONE |
| title | Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat |
| title_full | Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat |
| title_fullStr | Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat |
| title_full_unstemmed | Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat |
| title_short | Characterization of the pattern of expression of Gas1 in the kidney during postnatal development in the rat |
| title_sort | characterization of the pattern of expression of gas1 in the kidney during postnatal development in the rat |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124827/?tool=EBI |
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