Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers
The objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-01-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914609680 |
| _version_ | 1819103429891457024 |
|---|---|
| author | Qiu-feng ZENG Xia YANG Ping ZHENG Ke-ying ZHANG Yu-heng LUO Xue-mei DING Shi-ping BAI Jian-ping WANG Yue XUAN Zhuo-wei SU |
| author_facet | Qiu-feng ZENG Xia YANG Ping ZHENG Ke-ying ZHANG Yu-heng LUO Xue-mei DING Shi-ping BAI Jian-ping WANG Yue XUAN Zhuo-wei SU |
| author_sort | Qiu-feng ZENG |
| collection | DOAJ |
| description | The objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR-2) mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d: 1) LAT and a basal diet; 2) LAT and a basal diet supplemented with 1 000 mg kg−1 VC (LAT+VC); 3) normal ambient temperature (NAT) and a basal diet; 4) NAT and a basal diet supplemented with 1 000 mg kg−1 VC (NAT+VC). Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area (WA/TA, %) and mean media thickness in pulmonary arterioles (mMTPA, %) (P<0.05). Dietary VC supplementation decreased mMTPA (P<0.05), but had no effect on the WA/TA. LAT increased (P<0.05) the relative mRNA expression of HIF-1α, VEGF and VEGFR-2, while adding VC to the diet could decrease (P<0.05) their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT (P<0.05) or mMTPA (P<0.05). These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-1α, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression. |
| first_indexed | 2024-12-22T01:50:19Z |
| format | Article |
| id | doaj.art-b1c76c094fbb47ab95b9865172caad2d |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-22T01:50:19Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-b1c76c094fbb47ab95b9865172caad2d2022-12-21T18:42:57ZengElsevierJournal of Integrative Agriculture2095-31192016-01-01151183190Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilersQiu-feng ZENG0Xia YANG1Ping ZHENG2Ke-ying ZHANG3Yu-heng LUO4Xue-mei DING5Shi-ping BAI6Jian-ping WANG7Yue XUAN8Zhuo-wei SU9Correspondence ZENG Qiu-feng, Mobile: +86-13778765040, Fax: +86-835-2885065; Key Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R.ChinaThe objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR-2) mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d: 1) LAT and a basal diet; 2) LAT and a basal diet supplemented with 1 000 mg kg−1 VC (LAT+VC); 3) normal ambient temperature (NAT) and a basal diet; 4) NAT and a basal diet supplemented with 1 000 mg kg−1 VC (NAT+VC). Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area (WA/TA, %) and mean media thickness in pulmonary arterioles (mMTPA, %) (P<0.05). Dietary VC supplementation decreased mMTPA (P<0.05), but had no effect on the WA/TA. LAT increased (P<0.05) the relative mRNA expression of HIF-1α, VEGF and VEGFR-2, while adding VC to the diet could decrease (P<0.05) their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT (P<0.05) or mMTPA (P<0.05). These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-1α, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression.http://www.sciencedirect.com/science/article/pii/S2095311914609680broilerhypoxia inducible factor-1αlow ambient temperaturepulmonary vascular remodelingvitamin C |
| spellingShingle | Qiu-feng ZENG Xia YANG Ping ZHENG Ke-ying ZHANG Yu-heng LUO Xue-mei DING Shi-ping BAI Jian-ping WANG Yue XUAN Zhuo-wei SU Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers Journal of Integrative Agriculture broiler hypoxia inducible factor-1α low ambient temperature pulmonary vascular remodeling vitamin C |
| title | Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers |
| title_full | Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers |
| title_fullStr | Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers |
| title_full_unstemmed | Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers |
| title_short | Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers |
| title_sort | effects of low ambient temperatures and dietary vitamin c supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21 d old broilers |
| topic | broiler hypoxia inducible factor-1α low ambient temperature pulmonary vascular remodeling vitamin C |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914609680 |
| work_keys_str_mv | AT qiufengzeng effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT xiayang effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT pingzheng effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT keyingzhang effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT yuhengluo effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT xuemeiding effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT shipingbai effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT jianpingwang effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT yuexuan effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers AT zhuoweisu effectsoflowambienttemperaturesanddietaryvitamincsupplementationonpulmonaryvascularremodelingandhypoxicgeneexpressionof21doldbroilers |