First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco
Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Morocca...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-08-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3369 |
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| author | Abdelhamid Massik Lahbib Hibaoui Benboubker Moussa Ghita Yahyaoui Bouchra Oumokhtar Mustapha Mahmoud |
| author_facet | Abdelhamid Massik Lahbib Hibaoui Benboubker Moussa Ghita Yahyaoui Bouchra Oumokhtar Mustapha Mahmoud |
| author_sort | Abdelhamid Massik |
| collection | DOAJ |
| description |
Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Moroccan hospital.
Materials and Methods: Seventy carbapenem-resistant A. baumannii isolates from various samples were received at the microbiology laboratory of the Hospital Center. Antibiotic susceptibility was tested by the diffusion disc method and molecular characterization of antimicrobial resistance was performed by PCR and sequencing.
Results: Carbapenemase genes were detected in our isolates: the OXA-51 gene and the ISbA1 sequence were detected in all isolates (100%), the OXA-23 and OXA-58 genes were detected in 82.85% and 10% of isolates respectively, MBL genes were dominated by VIM 39 isolates (55.7%), followed by GIM 26 isolates (37%), SIM 20 isolates (28.5%), IMP 8 isolates (11, 4%), NDM 3 isolates (4%) and for the first time in Morocco SPM with 4 isolates (5.7%).
Conclusion: The emergence of resistance of A. baumannii to carbapenems is a serious problem in our hospital which requires the establishment of a prevention strategy and strict respect for hygiene to minimize their dissemination.
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| first_indexed | 2024-04-11T22:30:30Z |
| format | Article |
| id | doaj.art-b1c81756af1b4f0181a189fa8bae2bac |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-11T22:30:30Z |
| publishDate | 2022-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-b1c81756af1b4f0181a189fa8bae2bac2022-12-22T03:59:26ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-08-0114410.18502/ijm.v14i4.10229First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in MoroccoAbdelhamid Massik0Lahbib Hibaoui1Benboubker Moussa2Ghita Yahyaoui3Bouchra Oumokhtar4Mustapha Mahmoud5Laboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco; Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, MoroccoLaboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco; Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, MoroccoHuman Pathologies, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, MoroccoLaboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, MoroccoHuman Pathologies, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, MoroccoLaboratory of Microbiology and Molecular Biology, CHU Hassan II, Fez, Morocco; Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohammed Ben Abdellah University, Fez, Morocco Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Moroccan hospital. Materials and Methods: Seventy carbapenem-resistant A. baumannii isolates from various samples were received at the microbiology laboratory of the Hospital Center. Antibiotic susceptibility was tested by the diffusion disc method and molecular characterization of antimicrobial resistance was performed by PCR and sequencing. Results: Carbapenemase genes were detected in our isolates: the OXA-51 gene and the ISbA1 sequence were detected in all isolates (100%), the OXA-23 and OXA-58 genes were detected in 82.85% and 10% of isolates respectively, MBL genes were dominated by VIM 39 isolates (55.7%), followed by GIM 26 isolates (37%), SIM 20 isolates (28.5%), IMP 8 isolates (11, 4%), NDM 3 isolates (4%) and for the first time in Morocco SPM with 4 isolates (5.7%). Conclusion: The emergence of resistance of A. baumannii to carbapenems is a serious problem in our hospital which requires the establishment of a prevention strategy and strict respect for hygiene to minimize their dissemination. https://ijm.tums.ac.ir/index.php/ijm/article/view/3369Acinetobacter baumannii;Carbapenems;Resistance;Metallo-beta-lactamase |
| spellingShingle | Abdelhamid Massik Lahbib Hibaoui Benboubker Moussa Ghita Yahyaoui Bouchra Oumokhtar Mustapha Mahmoud First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco Iranian Journal of Microbiology Acinetobacter baumannii; Carbapenems; Resistance; Metallo-beta-lactamase |
| title | First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco |
| title_full | First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco |
| title_fullStr | First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco |
| title_full_unstemmed | First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco |
| title_short | First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco |
| title_sort | first report of spm metallo β lactamases producing acinetobacter baumannii isolates in morocco |
| topic | Acinetobacter baumannii; Carbapenems; Resistance; Metallo-beta-lactamase |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3369 |
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