CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME
Sarcoidosis is a disorder of unknown etiology characterized by development of necrosis-free epithelioid cell granulomas in various tissues. There are two main phenotypes of pulmonary sarcoidosis (PS): Lofgren's syndrome (LS) is an acute form with favorable outcome, while non-Lofgren's synd...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2019-03-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/1653 |
| _version_ | 1827850686719590400 |
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| author | Igor Vladimirovich Kudryavtsev Natalia Mihailovna Lazareva Olga Petrovna Baranova Alexey Sergeevich Golovkin Dmitry Valer’evich Isakov Maria Konstantinovna Serebriakova Tatyana Pavlovna Ses’ Mikhail Mikhailovich Ilkovich Areg Artemovich Totolian |
| author_facet | Igor Vladimirovich Kudryavtsev Natalia Mihailovna Lazareva Olga Petrovna Baranova Alexey Sergeevich Golovkin Dmitry Valer’evich Isakov Maria Konstantinovna Serebriakova Tatyana Pavlovna Ses’ Mikhail Mikhailovich Ilkovich Areg Artemovich Totolian |
| author_sort | Igor Vladimirovich Kudryavtsev |
| collection | DOAJ |
| description | Sarcoidosis is a disorder of unknown etiology characterized by development of necrosis-free epithelioid cell granulomas in various tissues. There are two main phenotypes of pulmonary sarcoidosis (PS): Lofgren's syndrome (LS) is an acute form with favorable outcome, while non-Lofgren's syndrome (nLS) is a chronic type of disease that can lead to pulmonary fibrosis in 20 % of cases. Our study was aimed at investigating changes in the main cell-surface differentiation antigens on peripheral blood regulatory T cells (Tregs) from patients with first diagnosed PS without treatment (LS (n=11) and nLS (n=46)) compared to healthy volunteers (HC, n=26), that might be used as immunological markers for predicting severity of this disorder. Flow cytometry analysis of peripheral blood cell samples demonstrated that patients with nLS had decreased relative number of CD3+ cells vs. HC as well as CD3+CD4+ cells vs. HC and LS patients. Furthermore, the relative and absolute number of Tregs were also decreased in nLS group vs. HC (2.83% (2.47; 3.36) vs. 3.33% (2.79; 3.84), p=0.021) and 37 (29; 52) cells vs. 50 (42; 65), p=0.004, respectively) per one microliter peripheral blood. A relative number of CD39-positive Тregs in chronic vs. acute sarcoidosis patients was associated with 51.02% (38.20; 61.62) vs. 48.64% (41.46; 63.72) that was significantly (p<0.001 and p=0.007, respectively) higher than in HC (39.52% (11.55; 46.34). It was found that “naïve” (CD45R0–CD62L+) Тregs did not significantly differ in percentage of CD39- and CD73-positive cells in all groups tested. Moreover, CD45R0+CD62L+ Тregs in LS and nLS patients contained significantly more CD39-positive cells (69.66% (61.92; 79.34) and 67.62% (61.92; 79.34), respectively, compared to 47.55% (15.74; 65.32) in HC (p<0.001 and p=0.004, respectively). In case of CD45R0+CD62L– Tregs able to exit from the circulation and migrate to the site of inflammation, an increased percentage of CD39-positive subset was noted only in patients with chronic sarcoidosis and HC (61.79% (55.12; 73.09) and 57.27% (16.03; 66.98), p=0.006). Enhanced CD39 expression on Tregs seems to be related to chronic immune response, so that antigen elimination becomes impossible due to Treg overactivation, as shown in patients with sarcoidosis and in some other chronic autoimmune and infectious disorders. |
| first_indexed | 2024-03-12T10:21:58Z |
| format | Article |
| id | doaj.art-b1c8b9bb81b74a08a52e0ac71519c019 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-03-12T10:21:58Z |
| publishDate | 2019-03-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-b1c8b9bb81b74a08a52e0ac71519c0192023-09-02T10:02:39ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2019-03-01001121CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROMEIgor Vladimirovich Kudryavtsev0Natalia Mihailovna Lazareva1Olga Petrovna Baranova2Alexey Sergeevich Golovkin3Dmitry Valer’evich Isakov4Maria Konstantinovna Serebriakova5Tatyana Pavlovna Ses’6Mikhail Mikhailovich Ilkovich7Areg Artemovich Totolian8Federal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian Federation; Federal State Budgetary Scientific Institution "Institute of Experimental Medicine" (FSBSI "IEM"), St.Petersburg, Russian FederationFederal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian FederationScientific Institute of Interstitial and Orphan Diseases; department of Pulmonology, Federal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian FederationInstitute of Molecular Biology and Genetics, V.A. Almazov National Medical Research Centre, St.Petersburg, Russian FederationFederal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian Federation; Federal State Budgetary Scientific Institution "Institute of Experimental Medicine" (FSBSI "IEM"), St.Petersburg, Russian FederationFederal State Budgetary Scientific Institution "Institute of Experimental Medicine" (FSBSI "IEM"), St.Petersburg, Russian FederationFederal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian FederationScientific Institute of Interstitial and Orphan Diseases; department of Pulmonology, Federal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian FederationFederal State Budgetary Educational Institution of Higher Education “Academician I.P. Pavlov First St. Petersburg State Medical University” of the Ministry of Healthcare of Russian Federation, St.Petersburg, Russian Federation; St. Petersburg Pasteur Institute, St. Petersburg, Russian FederationSarcoidosis is a disorder of unknown etiology characterized by development of necrosis-free epithelioid cell granulomas in various tissues. There are two main phenotypes of pulmonary sarcoidosis (PS): Lofgren's syndrome (LS) is an acute form with favorable outcome, while non-Lofgren's syndrome (nLS) is a chronic type of disease that can lead to pulmonary fibrosis in 20 % of cases. Our study was aimed at investigating changes in the main cell-surface differentiation antigens on peripheral blood regulatory T cells (Tregs) from patients with first diagnosed PS without treatment (LS (n=11) and nLS (n=46)) compared to healthy volunteers (HC, n=26), that might be used as immunological markers for predicting severity of this disorder. Flow cytometry analysis of peripheral blood cell samples demonstrated that patients with nLS had decreased relative number of CD3+ cells vs. HC as well as CD3+CD4+ cells vs. HC and LS patients. Furthermore, the relative and absolute number of Tregs were also decreased in nLS group vs. HC (2.83% (2.47; 3.36) vs. 3.33% (2.79; 3.84), p=0.021) and 37 (29; 52) cells vs. 50 (42; 65), p=0.004, respectively) per one microliter peripheral blood. A relative number of CD39-positive Тregs in chronic vs. acute sarcoidosis patients was associated with 51.02% (38.20; 61.62) vs. 48.64% (41.46; 63.72) that was significantly (p<0.001 and p=0.007, respectively) higher than in HC (39.52% (11.55; 46.34). It was found that “naïve” (CD45R0–CD62L+) Тregs did not significantly differ in percentage of CD39- and CD73-positive cells in all groups tested. Moreover, CD45R0+CD62L+ Тregs in LS and nLS patients contained significantly more CD39-positive cells (69.66% (61.92; 79.34) and 67.62% (61.92; 79.34), respectively, compared to 47.55% (15.74; 65.32) in HC (p<0.001 and p=0.004, respectively). In case of CD45R0+CD62L– Tregs able to exit from the circulation and migrate to the site of inflammation, an increased percentage of CD39-positive subset was noted only in patients with chronic sarcoidosis and HC (61.79% (55.12; 73.09) and 57.27% (16.03; 66.98), p=0.006). Enhanced CD39 expression on Tregs seems to be related to chronic immune response, so that antigen elimination becomes impossible due to Treg overactivation, as shown in patients with sarcoidosis and in some other chronic autoimmune and infectious disorders.https://www.mimmun.ru/mimmun/article/view/1653sarcoidosis, regulatory T cells, CD39 expression, flow cytometrymulticolor immunophenotyping |
| spellingShingle | Igor Vladimirovich Kudryavtsev Natalia Mihailovna Lazareva Olga Petrovna Baranova Alexey Sergeevich Golovkin Dmitry Valer’evich Isakov Maria Konstantinovna Serebriakova Tatyana Pavlovna Ses’ Mikhail Mikhailovich Ilkovich Areg Artemovich Totolian CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME Медицинская иммунология sarcoidosis, regulatory T cells, CD39 expression, flow cytometry multicolor immunophenotyping |
| title | CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME |
| title_full | CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME |
| title_fullStr | CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME |
| title_full_unstemmed | CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME |
| title_short | CD39+ REGULATORY T CELLS IN PULMONARY SARCOIDOSIS AND LOFGREN'S SYNDROME |
| title_sort | cd39 regulatory t cells in pulmonary sarcoidosis and lofgren s syndrome |
| topic | sarcoidosis, regulatory T cells, CD39 expression, flow cytometry multicolor immunophenotyping |
| url | https://www.mimmun.ru/mimmun/article/view/1653 |
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