Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study.
Besides its role in controlling the morphology of mitochondria, mitofusin-2 has been proposed to tether mitochondria to the endoplasmic reticulum (ER), based largely on light microscopic analysis. In this study we have examined by electron microscopy the organization of ER and mitochondria in cells...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3460865?pdf=render |
| _version_ | 1818954805490483200 |
|---|---|
| author | Pierre Cosson Anna Marchetti Mariella Ravazzola Lelio Orci |
| author_facet | Pierre Cosson Anna Marchetti Mariella Ravazzola Lelio Orci |
| author_sort | Pierre Cosson |
| collection | DOAJ |
| description | Besides its role in controlling the morphology of mitochondria, mitofusin-2 has been proposed to tether mitochondria to the endoplasmic reticulum (ER), based largely on light microscopic analysis. In this study we have examined by electron microscopy the organization of ER and mitochondria in cells expressing or not mitofusin-2. Contrary to previous studies, we observed that loss of mitofusin-2 increased ER-mitochondria juxtaposition. These results suggest that mitofusin-2 does not play a critical role in the juxtapostion of ER and mitochondria, and highlight the essential role of ultrastructural analysis to visualize and measure contact between two intracellular compartments. |
| first_indexed | 2024-12-20T10:28:00Z |
| format | Article |
| id | doaj.art-b1c9a214dff2453997ac5e6af6d6c84a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T10:28:00Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b1c9a214dff2453997ac5e6af6d6c84a2022-12-21T19:43:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4629310.1371/journal.pone.0046293Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study.Pierre CossonAnna MarchettiMariella RavazzolaLelio OrciBesides its role in controlling the morphology of mitochondria, mitofusin-2 has been proposed to tether mitochondria to the endoplasmic reticulum (ER), based largely on light microscopic analysis. In this study we have examined by electron microscopy the organization of ER and mitochondria in cells expressing or not mitofusin-2. Contrary to previous studies, we observed that loss of mitofusin-2 increased ER-mitochondria juxtaposition. These results suggest that mitofusin-2 does not play a critical role in the juxtapostion of ER and mitochondria, and highlight the essential role of ultrastructural analysis to visualize and measure contact between two intracellular compartments.http://europepmc.org/articles/PMC3460865?pdf=render |
| spellingShingle | Pierre Cosson Anna Marchetti Mariella Ravazzola Lelio Orci Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study. PLoS ONE |
| title | Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study. |
| title_full | Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study. |
| title_fullStr | Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study. |
| title_full_unstemmed | Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study. |
| title_short | Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study. |
| title_sort | mitofusin 2 independent juxtaposition of endoplasmic reticulum and mitochondria an ultrastructural study |
| url | http://europepmc.org/articles/PMC3460865?pdf=render |
| work_keys_str_mv | AT pierrecosson mitofusin2independentjuxtapositionofendoplasmicreticulumandmitochondriaanultrastructuralstudy AT annamarchetti mitofusin2independentjuxtapositionofendoplasmicreticulumandmitochondriaanultrastructuralstudy AT mariellaravazzola mitofusin2independentjuxtapositionofendoplasmicreticulumandmitochondriaanultrastructuralstudy AT lelioorci mitofusin2independentjuxtapositionofendoplasmicreticulumandmitochondriaanultrastructuralstudy |