Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2
Abstract Background Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO2. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products. Results In...
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| Natura: | Articolo |
| Lingua: | English |
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BMC
2019-01-01
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| Serie: | Microbial Cell Factories |
| Soggetti: | |
| Accesso online: | http://link.springer.com/article/10.1186/s12934-019-1067-3 |
| _version_ | 1829514660960796672 |
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| author | Zaiqiang Wu Junsong Wang Jun Liu Yan Wang Changhao Bi Xueli Zhang |
| author_facet | Zaiqiang Wu Junsong Wang Jun Liu Yan Wang Changhao Bi Xueli Zhang |
| author_sort | Zaiqiang Wu |
| collection | DOAJ |
| description | Abstract Background Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO2. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products. Results In this research, an electroactive succinate-producing cell factory was engineered in E. coli T110(pMtrABC, pFccA-CymA) by expressing mtrABC, fccA and cymA from Shewanella oneidensis MR-1, which can utilize electricity to reduce fumarate. The electroactive T110 strain was further improved by incorporating a carbon concentration mechanism (CCM). This strain was fermented in an MES system with neutral red as the electron carrier and supplemented with HCO3 +, which produced a succinate yield of 1.10 mol/mol glucose—a 1.6-fold improvement over the parent strain T110. Conclusions The strain T110(pMtrABC, pFccA-CymA, pBTCA) is to our best knowledge the first electroactive microbial cell factory engineered to directly utilize electricity for the production of a specific product. Due to the versatility of the E. coli platform, this pioneering research opens the possibility of engineering various other cell factories to utilize electricity for bioproduction. |
| first_indexed | 2024-12-16T13:22:53Z |
| format | Article |
| id | doaj.art-b1c9dbe392c942618bd9a6d33d726ed9 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-16T13:22:53Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-b1c9dbe392c942618bd9a6d33d726ed92022-12-21T22:30:19ZengBMCMicrobial Cell Factories1475-28592019-01-0118111410.1186/s12934-019-1067-3Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2Zaiqiang Wu0Junsong Wang1Jun Liu2Yan Wang3Changhao Bi4Xueli Zhang5Center for Molecular Metabolism, School of Environmental and Biological Engineering, Nanjing University of Science and TechnologyCenter for Molecular Metabolism, School of Environmental and Biological Engineering, Nanjing University of Science and TechnologyKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesAbstract Background Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO2. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products. Results In this research, an electroactive succinate-producing cell factory was engineered in E. coli T110(pMtrABC, pFccA-CymA) by expressing mtrABC, fccA and cymA from Shewanella oneidensis MR-1, which can utilize electricity to reduce fumarate. The electroactive T110 strain was further improved by incorporating a carbon concentration mechanism (CCM). This strain was fermented in an MES system with neutral red as the electron carrier and supplemented with HCO3 +, which produced a succinate yield of 1.10 mol/mol glucose—a 1.6-fold improvement over the parent strain T110. Conclusions The strain T110(pMtrABC, pFccA-CymA, pBTCA) is to our best knowledge the first electroactive microbial cell factory engineered to directly utilize electricity for the production of a specific product. Due to the versatility of the E. coli platform, this pioneering research opens the possibility of engineering various other cell factories to utilize electricity for bioproduction.http://link.springer.com/article/10.1186/s12934-019-1067-3Microbial electrosynthesisBioelectrochemical systemsSuccinateCO2 fixation |
| spellingShingle | Zaiqiang Wu Junsong Wang Jun Liu Yan Wang Changhao Bi Xueli Zhang Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2 Microbial Cell Factories Microbial electrosynthesis Bioelectrochemical systems Succinate CO2 fixation |
| title | Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2 |
| title_full | Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2 |
| title_fullStr | Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2 |
| title_full_unstemmed | Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2 |
| title_short | Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2 |
| title_sort | engineering an electroactive escherichia coli for the microbial electrosynthesis of succinate from glucose and co2 |
| topic | Microbial electrosynthesis Bioelectrochemical systems Succinate CO2 fixation |
| url | http://link.springer.com/article/10.1186/s12934-019-1067-3 |
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