Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues
Breast cancer is one of the main global priorities in terms of public health. It remains the most frequent cancer in women and is the leading cause of their death. The human microbiome plays various roles in maintaining health by ensuring a dynamic balance with the host or in the appearance of vario...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/13/3/450 |
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| author | Souad Kartti Houda Bendani Nasma Boumajdi El Mehdi Bouricha Oumaima Zarrik Hajar EL Agouri Mohamed Fokar Youssef Aghlallou Rachid EL Jaoudi Lahcen Belyamani Basma Elkhannoussi Azeddine Ibrahimi |
| author_facet | Souad Kartti Houda Bendani Nasma Boumajdi El Mehdi Bouricha Oumaima Zarrik Hajar EL Agouri Mohamed Fokar Youssef Aghlallou Rachid EL Jaoudi Lahcen Belyamani Basma Elkhannoussi Azeddine Ibrahimi |
| author_sort | Souad Kartti |
| collection | DOAJ |
| description | Breast cancer is one of the main global priorities in terms of public health. It remains the most frequent cancer in women and is the leading cause of their death. The human microbiome plays various roles in maintaining health by ensuring a dynamic balance with the host or in the appearance of various pathologies including breast cancer. In this study, we performed an analysis of bacterial signature differences between tumor and adjacent tissues of breast cancer patients in Morocco. Using 16S rRNA gene sequencing, we observed that adjacent tissue contained a much higher percentage of the Gammaproteobacteria class (35.7%) while tumor tissue was characterized by a higher percentage of Bacilli and Actinobacteria classes, with about 18.8% and 17.2% average abundance, respectively. Analysis of tumor subtype revealed enrichment of genus Sphingomonodas in TNBC while Sphingomonodas was predominant in HER2. The LEfSe and the genus level heatmap analysis revealed a higher abundance of the Rothia genus in tumor tissues. The identified microbial communities can therefore serve as potential biomarkers for prognosis and diagnosis, while also helping to develop new strategies for the treatment of breast cancer patients. |
| first_indexed | 2024-03-11T06:19:27Z |
| format | Article |
| id | doaj.art-b1ca146e5c7a47d7945675defae283db |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-11T06:19:27Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-b1ca146e5c7a47d7945675defae283db2023-11-17T12:02:31ZengMDPI AGJournal of Personalized Medicine2075-44262023-02-0113345010.3390/jpm13030450Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor TissuesSouad Kartti0Houda Bendani1Nasma Boumajdi2El Mehdi Bouricha3Oumaima Zarrik4Hajar EL Agouri5Mohamed Fokar6Youssef Aghlallou7Rachid EL Jaoudi8Lahcen Belyamani9Basma Elkhannoussi10Azeddine Ibrahimi11Biotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoBiotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoBiotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoBiotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoBiotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoPathology Department, Oncology National Institute, Rabat Medical and Pharmacy School, Mohammed Vth University in Rabat, Rabat 10100, MoroccoCenter for Biotechnology and Genomics, Texas Tech University, Lubbock, TX 79409, USAInstitute of Cancer Research, Fez 30000, MoroccoBiotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoMohammed VI Center for Research & Innovation (CM6), Rabat 10000, MoroccoPathology Department, Oncology National Institute, Rabat Medical and Pharmacy School, Mohammed Vth University in Rabat, Rabat 10100, MoroccoBiotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, MoroccoBreast cancer is one of the main global priorities in terms of public health. It remains the most frequent cancer in women and is the leading cause of their death. The human microbiome plays various roles in maintaining health by ensuring a dynamic balance with the host or in the appearance of various pathologies including breast cancer. In this study, we performed an analysis of bacterial signature differences between tumor and adjacent tissues of breast cancer patients in Morocco. Using 16S rRNA gene sequencing, we observed that adjacent tissue contained a much higher percentage of the Gammaproteobacteria class (35.7%) while tumor tissue was characterized by a higher percentage of Bacilli and Actinobacteria classes, with about 18.8% and 17.2% average abundance, respectively. Analysis of tumor subtype revealed enrichment of genus Sphingomonodas in TNBC while Sphingomonodas was predominant in HER2. The LEfSe and the genus level heatmap analysis revealed a higher abundance of the Rothia genus in tumor tissues. The identified microbial communities can therefore serve as potential biomarkers for prognosis and diagnosis, while also helping to develop new strategies for the treatment of breast cancer patients.https://www.mdpi.com/2075-4426/13/3/450breast cancermetagenomics16s rRNA sequencingmicrobiomerhotia |
| spellingShingle | Souad Kartti Houda Bendani Nasma Boumajdi El Mehdi Bouricha Oumaima Zarrik Hajar EL Agouri Mohamed Fokar Youssef Aghlallou Rachid EL Jaoudi Lahcen Belyamani Basma Elkhannoussi Azeddine Ibrahimi Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues Journal of Personalized Medicine breast cancer metagenomics 16s rRNA sequencing microbiome rhotia |
| title | Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues |
| title_full | Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues |
| title_fullStr | Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues |
| title_full_unstemmed | Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues |
| title_short | Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues |
| title_sort | metagenomics analysis of breast microbiome highlights the abundance of rothia genus in tumor tissues |
| topic | breast cancer metagenomics 16s rRNA sequencing microbiome rhotia |
| url | https://www.mdpi.com/2075-4426/13/3/450 |
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