Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts
Salmonella enterica (S. enterica) is a major foodborne pathogen leading to a large number of outbreaks and bringing food safety concerns to sprouts. The control of S. enterica on maize sprouts is important because raw maize sprouts have been gaining attention as a novel superfood. Compared to conven...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927124000340 |
| _version_ | 1827221802692116480 |
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| author | Nan Xiang Catherine W.Y. Wong Xinbo Guo Siyun Wang |
| author_facet | Nan Xiang Catherine W.Y. Wong Xinbo Guo Siyun Wang |
| author_sort | Nan Xiang |
| collection | DOAJ |
| description | Salmonella enterica (S. enterica) is a major foodborne pathogen leading to a large number of outbreaks and bringing food safety concerns to sprouts. The control of S. enterica on maize sprouts is important because raw maize sprouts have been gaining attention as a novel superfood. Compared to conventional chemical methods, the applications of bacteriophages are regarded as natural and organic. This study investigated the effects of a 2 h phage cocktail (SF1 and SI1, MOI 1000) soaking on reducing the populations of three Salmonella enterica strains: S. Enteritidis S5-483, S. Typhimurium S5-536, and S. Agona PARC5 on maize seeds and during the storage of maize sprouts. The results showed that the phage cocktail treatment effectively reduced populations of S. enterica strains by 1–3 log CFU/g on maize seeds and decreased population of S. Agona PACR5 by 1.16 log CFU/g on maize sprouts from 7.55 log CFU/g at day 0 of the storage period. On the other hand, the upregulations of flagella gene pefA by 1.5-folds and membrane gene lpxA by 23-folds in S. Typhimurium S5-536 indicated a differential response to the phage cocktail treatment. Conversely, stress response genes ompR, rpoS, and recA, as well as the DNA repair gene yafD, were downregulated in S. Agona PARC5. This work shows the use of bacteriophages could contribute as a part of hurdle effect to reduce S. enterica populations and is beneficial to develop strategies for controlling foodborne pathogens in the production and storage of maize sprouts. |
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| id | doaj.art-b1ca1734319a4311a9091638aa581ecd |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2025-03-21T16:28:23Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-b1ca1734319a4311a9091638aa581ecd2024-06-17T05:57:37ZengElsevierCurrent Research in Food Science2665-92712024-01-018100708Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sproutsNan Xiang0Catherine W.Y. Wong1Xinbo Guo2Siyun Wang3School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou, 510640, China; Food, Nutrition and Health, University of British Columbia, 120-2205 East Mall, Vancouver, BC, V6R 1Z4, CanadaFood, Nutrition and Health, University of British Columbia, 120-2205 East Mall, Vancouver, BC, V6R 1Z4, CanadaSchool of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou, 510640, ChinaFood, Nutrition and Health, University of British Columbia, 120-2205 East Mall, Vancouver, BC, V6R 1Z4, Canada; Corresponding author.Salmonella enterica (S. enterica) is a major foodborne pathogen leading to a large number of outbreaks and bringing food safety concerns to sprouts. The control of S. enterica on maize sprouts is important because raw maize sprouts have been gaining attention as a novel superfood. Compared to conventional chemical methods, the applications of bacteriophages are regarded as natural and organic. This study investigated the effects of a 2 h phage cocktail (SF1 and SI1, MOI 1000) soaking on reducing the populations of three Salmonella enterica strains: S. Enteritidis S5-483, S. Typhimurium S5-536, and S. Agona PARC5 on maize seeds and during the storage of maize sprouts. The results showed that the phage cocktail treatment effectively reduced populations of S. enterica strains by 1–3 log CFU/g on maize seeds and decreased population of S. Agona PACR5 by 1.16 log CFU/g on maize sprouts from 7.55 log CFU/g at day 0 of the storage period. On the other hand, the upregulations of flagella gene pefA by 1.5-folds and membrane gene lpxA by 23-folds in S. Typhimurium S5-536 indicated a differential response to the phage cocktail treatment. Conversely, stress response genes ompR, rpoS, and recA, as well as the DNA repair gene yafD, were downregulated in S. Agona PARC5. This work shows the use of bacteriophages could contribute as a part of hurdle effect to reduce S. enterica populations and is beneficial to develop strategies for controlling foodborne pathogens in the production and storage of maize sprouts.http://www.sciencedirect.com/science/article/pii/S2665927124000340Salmonella entericaMaize sproutsBacteriophageGene expressionSeedsStorage |
| spellingShingle | Nan Xiang Catherine W.Y. Wong Xinbo Guo Siyun Wang Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts Current Research in Food Science Salmonella enterica Maize sprouts Bacteriophage Gene expression Seeds Storage |
| title | Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts |
| title_full | Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts |
| title_fullStr | Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts |
| title_full_unstemmed | Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts |
| title_short | Infectivity responses of Salmonella enterica to bacteriophages on maize seeds and maize sprouts |
| title_sort | infectivity responses of salmonella enterica to bacteriophages on maize seeds and maize sprouts |
| topic | Salmonella enterica Maize sprouts Bacteriophage Gene expression Seeds Storage |
| url | http://www.sciencedirect.com/science/article/pii/S2665927124000340 |
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